Evaluation of the genotoxic effects of herbicide 2,4-D in Piaractus mesopotamicus by micronucleus test

The herbicide 2,4-D is currently one of the most used agrochemicals in agriculture. The fish are target of contamination, these when being in contact with toxics develop later alterations that can be studied, reason why they are used as models in the evaluation of aquatic ecosystems. There is scarce...

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Autores principales: Cowper, C. F., Jorge, M. J., Jorge, L. C.
Formato: Artículo revista
Lenguaje:Inglés
Publicado: Universidad Nacional del Nordeste 2021
Materias:
Piaractus mesoptamicus
2.4-D
mutagenicidad
micronucleos y alteraciones morfológicas nucleares
mutagenicity
micronucleus and alteration in the shape of nuclei
Acceso en línea:https://revistas.unne.edu.ar/index.php/vet/article/view/4739
Aporte de:
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) de Universidad Nacional del Nordeste
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institution Universidad Nacional del Nordeste
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container_title_str Revistas UNNE - Universidad Nacional del Noroeste (UNNE)
language Inglés
format Artículo revista
topic Piaractus mesoptamicus
2.4-D
mutagenicidad
micronucleos y alteraciones morfológicas nucleares
Piaractus mesoptamicus
2.4-D
mutagenicity
micronucleus and alteration in the shape of nuclei
spellingShingle Piaractus mesoptamicus
2.4-D
mutagenicidad
micronucleos y alteraciones morfológicas nucleares
Piaractus mesoptamicus
2.4-D
mutagenicity
micronucleus and alteration in the shape of nuclei
Cowper, C. F.
Jorge, M. J.
Jorge, L. C.
Evaluation of the genotoxic effects of herbicide 2,4-D in Piaractus mesopotamicus by micronucleus test
topic_facet Piaractus mesoptamicus
2.4-D
mutagenicidad
micronucleos y alteraciones morfológicas nucleares
Piaractus mesoptamicus
2.4-D
mutagenicity
micronucleus and alteration in the shape of nuclei
author Cowper, C. F.
Jorge, M. J.
Jorge, L. C.
author_facet Cowper, C. F.
Jorge, M. J.
Jorge, L. C.
author_sort Cowper, C. F.
title Evaluation of the genotoxic effects of herbicide 2,4-D in Piaractus mesopotamicus by micronucleus test
title_short Evaluation of the genotoxic effects of herbicide 2,4-D in Piaractus mesopotamicus by micronucleus test
title_full Evaluation of the genotoxic effects of herbicide 2,4-D in Piaractus mesopotamicus by micronucleus test
title_fullStr Evaluation of the genotoxic effects of herbicide 2,4-D in Piaractus mesopotamicus by micronucleus test
title_full_unstemmed Evaluation of the genotoxic effects of herbicide 2,4-D in Piaractus mesopotamicus by micronucleus test
title_sort evaluation of the genotoxic effects of herbicide 2,4-d in piaractus mesopotamicus by micronucleus test
description The herbicide 2,4-D is currently one of the most used agrochemicals in agriculture. The fish are target of contamination, these when being in contact with toxics develop later alterations that can be studied, reason why they are used as models in the evaluation of aquatic ecosystems. There is scarce information about the effects of these pesticides in fish. Because of this, the objective of this work was to evaluate the possible impact of a contamination with 2,4-D, in sub-lethal concentrations in Piaractus mesopotamicus, through the frequency of micronuclei (MN) and alterations in the shape of the nuclei (NMA) in peripheral blood erythrocytes in chronic conditions (70 days). Two trials were conducted, one with the herbicide 2,4-D pure (P) and another with a commercial formulation (2,4-D bitter amine) (FC). Each experience was composed of five aquariums with two specimens in each, where different concentrations of the pesticide were administered (1 ppm, 1.8 ppm, 3.2 ppm, 5.6 ppm and 10 ppm), and in another with well water (control). A total of 4000 cells per individual were analyzed. Through the test MN and NMA the presence of diverse nuclear alterations was evidenced. The mentioned test for P and dilutions 1 ppm, 1.8 ppm, 3.2 ppm, 5.6 ppm of FC did not show significant differences with the control, while the concentration of 10 ppm of FC differed statistically from its control, this could be due to additional components in FC.
publisher Universidad Nacional del Nordeste
publishDate 2021
url https://revistas.unne.edu.ar/index.php/vet/article/view/4739
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AT jorgelc evaluationofthegenotoxiceffectsofherbicide24dinpiaractusmesopotamicusbymicronucleustest
AT cowpercf evaluaciondelosefectosgenotoxicosdelherbicida24denpiaractusmesopotamicusatravesdeltestdemicronucleos
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first_indexed 2025-05-17T05:10:14Z
last_indexed 2025-05-17T05:10:14Z
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spelling I48-R154-article-47392025-02-12T21:59:24Z Evaluation of the genotoxic effects of herbicide 2,4-D in Piaractus mesopotamicus by micronucleus test Evaluación de los efectos genotóxicos del herbicida 2,4-D en Piaractus mesopotamicus a través del test de micronúcleos Cowper, C. F. Jorge, M. J. Jorge, L. C. Piaractus mesoptamicus 2.4-D mutagenicidad micronucleos y alteraciones morfológicas nucleares Piaractus mesoptamicus 2.4-D mutagenicity micronucleus and alteration in the shape of nuclei The herbicide 2,4-D is currently one of the most used agrochemicals in agriculture. The fish are target of contamination, these when being in contact with toxics develop later alterations that can be studied, reason why they are used as models in the evaluation of aquatic ecosystems. There is scarce information about the effects of these pesticides in fish. Because of this, the objective of this work was to evaluate the possible impact of a contamination with 2,4-D, in sub-lethal concentrations in Piaractus mesopotamicus, through the frequency of micronuclei (MN) and alterations in the shape of the nuclei (NMA) in peripheral blood erythrocytes in chronic conditions (70 days). Two trials were conducted, one with the herbicide 2,4-D pure (P) and another with a commercial formulation (2,4-D bitter amine) (FC). Each experience was composed of five aquariums with two specimens in each, where different concentrations of the pesticide were administered (1 ppm, 1.8 ppm, 3.2 ppm, 5.6 ppm and 10 ppm), and in another with well water (control). A total of 4000 cells per individual were analyzed. Through the test MN and NMA the presence of diverse nuclear alterations was evidenced. The mentioned test for P and dilutions 1 ppm, 1.8 ppm, 3.2 ppm, 5.6 ppm of FC did not show significant differences with the control, while the concentration of 10 ppm of FC differed statistically from its control, this could be due to additional components in FC. Los herbicidas están siendo abundantemente utilizados haciendo que los mismos no solo lleguen al organismo específico sino también contaminen el medio ambiente donde son empleados. El herbicida 2,4-D es en la actualidad uno de los agroquímicos más utilizado en la agricultura. Los peces son blanco de la contaminación, éstos al estar en contacto con tóxicos desarrollan posteriormente alteraciones que pueden ser estudiadas, por lo cual son utilizados como modelos en la evaluacion de ecosistemas acuáticos. Existe escasa información acerca de los efectos de estos pesticidas en peces, siendo el actual trabajo el primero en evaluar efectos crónicos. El objetivo de la presente investigación fue evaluar el posible impacto de una contaminación con 2,4-D en concentraciones subletales en Piaractus mesopotamicus, a través de la frecuencia de micronúcleos (MN) y de las alteraciones en la forma de los núcleos (NMA) en eritrocitos de sangre periférica en condiciones crónicas (70 días). Se realizaron dos ensayos, uno con el herbicida 2,4-D puro (P) y otro con una formulación comercial (2,4-D amina Sumargo) (FC). Cada experiencia estuvo compuesta por cinco acuarios con dos ejemplares en cada uno, donde se administraron difererentes concentraciones del plaguicida (1 ppm, 1,8 ppm, 3,2 ppm, 5,6 ppm y 10 ppm), y en otra con agua de pozo (control). Se analizó un total de 4000 células por individuo. A través del test MN y NMA se evidenció la presencia de diversas alteraciones nucleares. El mencionado test para P y las diluciones 1 ppm, 1,8 ppm, 3,2 ppm, 5,6 ppm de FC no mostraron diferencias significativas con el control, mientras que la concentración de 10 ppm de FC se diferenció estadísticamente de su control. El presente trabajo aporta luz de los potenciales efectos nocivos del 2,4-D en el medio ambiente y posibilita nuevos estudios prospectivos y retrospectivos. Universidad Nacional del Nordeste 2021-04-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/4739 10.30972/vet.3124739 Revista Veterinaria; Vol. 31 Núm. 2 (2020); 165-170 1669-6840 1668-4834 eng https://revistas.unne.edu.ar/index.php/vet/article/view/4739/4438 Derechos de autor 2021 C. F. Cowper, M. J. Jorge, L. C. Jorge https://creativecommons.org/licenses/by-nc/4.0

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