Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose

The aim of the present study was to evaluate the relationship between intestinal microbial populations and bacterial translocation in young calves supplemented with lactic acid bacteria (LAB) and lactose and experimentally infected with Salmonella dublin DSPV 595T. Fifteen calves were divided into t...

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Autores principales: Frizzo, Laureano S., Soto, L.P., Bertozzi, E., Zbrun, María Virginia, Signorini Porchietto, Marcelo Lisandro, Sequeira, Gabriel Jorge, Rodríguez Armesto, R., Rosmini, Marcelo
Formato: Artículo PeerReviewed
Lenguaje:Español
Publicado: 2011
Materias:
QD Química
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/5019/1/A_Frizzo.pdf
Aporte de:
Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-5019
record_format dspace
spelling I38-R144-50192025-11-13T13:48:34Z http://pa.bibdigital.ucc.edu.ar/5019/ Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose Frizzo, Laureano S. Soto, L.P. Bertozzi, E. Zbrun, María Virginia Signorini Porchietto, Marcelo Lisandro Sequeira, Gabriel Jorge Rodríguez Armesto, R. Rosmini, Marcelo QD Química The aim of the present study was to evaluate the relationship between intestinal microbial populations and bacterial translocation in young calves supplemented with lactic acid bacteria (LAB) and lactose and experimentally infected with Salmonella dublin DSPV 595T. Fifteen calves were divided into three groups (a control group, a group inoculated with LAB, and a group inoculated with LAB and lactose), with 5, 6 and 4 animals each, were used. The LAB inoculum, composed of Lactobacillus casei DSPV 318T, L. salivarius DSPV 315T and Pediococcus acidilactici DSPV 006T was administered together with the milk replacer. The groups inoculated with LAB and LAB and lactose received a daily dose of 109CFU/kg body weight of each strain throughout the experiment. Lactose was provided in doses of 100g/d. The administration of the pathogen was performed on all animals on day 11 of the experiment with 2×1010CFU. The levels of probiotic inoculum found in the large intestine of the animals from the two groups inoculated with the probiotic [0] were of 5log10CFU/g, whereas those in the small intestine were of 5log10CFU/g in the animals inoculated with LAB and lactose, and approximately 1log10CFU/g less in the group inoculated with LAB. The levels of Salmonella in both, the small and large intestines, were between 3log10CFU/g and 4log10CFU/g. High microbial loads were found in the internal organs. Lactobacillus spp. were found only in the lymph nodes of the two groups inoculated with the probiotic, in very low amounts (<1log10CFU/g). Despite the high concentration of Salmonella administered to calves, the LAB inoculum of bovine origin was not capable of translocating to the internal organs in the extreme situations of intestinal imbalance generated by the pathogen. 2011-12-31 Artículo PeerReviewed application/pdf spa http://pa.bibdigital.ucc.edu.ar/5019/1/A_Frizzo.pdf Frizzo, Laureano S. ORCID: https://orcid.org/0000-0002-4354-4564 <https://orcid.org/0000-0002-4354-4564>, Soto, L.P., Bertozzi, E., Zbrun, María Virginia ORCID: https://orcid.org/0000-0001-7438-9803 <https://orcid.org/0000-0001-7438-9803>, Signorini Porchietto, Marcelo Lisandro, Sequeira, Gabriel Jorge ORCID: https://orcid.org/0000-0003-1332-7988 <https://orcid.org/0000-0003-1332-7988>, Rodríguez Armesto, R. and Rosmini, Marcelo ORCID: https://orcid.org/0000-0002-4171-411X <https://orcid.org/0000-0002-4171-411X> (2011) Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose. Animal Feed Science and Technology, 170. pp. 12-20. ISSN 03778401 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anifeedsci.2011.07.016
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic QD Química
spellingShingle QD Química
Frizzo, Laureano S.
Soto, L.P.
Bertozzi, E.
Zbrun, María Virginia
Signorini Porchietto, Marcelo Lisandro
Sequeira, Gabriel Jorge
Rodríguez Armesto, R.
Rosmini, Marcelo
Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose
topic_facet QD Química
description The aim of the present study was to evaluate the relationship between intestinal microbial populations and bacterial translocation in young calves supplemented with lactic acid bacteria (LAB) and lactose and experimentally infected with Salmonella dublin DSPV 595T. Fifteen calves were divided into three groups (a control group, a group inoculated with LAB, and a group inoculated with LAB and lactose), with 5, 6 and 4 animals each, were used. The LAB inoculum, composed of Lactobacillus casei DSPV 318T, L. salivarius DSPV 315T and Pediococcus acidilactici DSPV 006T was administered together with the milk replacer. The groups inoculated with LAB and LAB and lactose received a daily dose of 109CFU/kg body weight of each strain throughout the experiment. Lactose was provided in doses of 100g/d. The administration of the pathogen was performed on all animals on day 11 of the experiment with 2×1010CFU. The levels of probiotic inoculum found in the large intestine of the animals from the two groups inoculated with the probiotic [0] were of 5log10CFU/g, whereas those in the small intestine were of 5log10CFU/g in the animals inoculated with LAB and lactose, and approximately 1log10CFU/g less in the group inoculated with LAB. The levels of Salmonella in both, the small and large intestines, were between 3log10CFU/g and 4log10CFU/g. High microbial loads were found in the internal organs. Lactobacillus spp. were found only in the lymph nodes of the two groups inoculated with the probiotic, in very low amounts (<1log10CFU/g). Despite the high concentration of Salmonella administered to calves, the LAB inoculum of bovine origin was not capable of translocating to the internal organs in the extreme situations of intestinal imbalance generated by the pathogen.
format Artículo
PeerReviewed
author Frizzo, Laureano S.
Soto, L.P.
Bertozzi, E.
Zbrun, María Virginia
Signorini Porchietto, Marcelo Lisandro
Sequeira, Gabriel Jorge
Rodríguez Armesto, R.
Rosmini, Marcelo
author_facet Frizzo, Laureano S.
Soto, L.P.
Bertozzi, E.
Zbrun, María Virginia
Signorini Porchietto, Marcelo Lisandro
Sequeira, Gabriel Jorge
Rodríguez Armesto, R.
Rosmini, Marcelo
author_sort Frizzo, Laureano S.
title Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose
title_short Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose
title_full Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose
title_fullStr Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose
title_full_unstemmed Intestinal populations of Lactobacilli and coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose
title_sort intestinal populations of lactobacilli and coliforms after in vivo salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose
publishDate 2011
url http://pa.bibdigital.ucc.edu.ar/5019/1/A_Frizzo.pdf
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