Acanthamoeba sp. keratitis: First case confirmed by isolation and molecular typification in Bahía Blanca, Buenos Aires Province, Argentina [Queratitis por Acanthamoeba sp.: Primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina]

Some species of the Acanthamoeba genus cause keratitis, a very painful, most likely unilateral corneal infection, associated with eye and vision impairment. We here present a case of a 31-year-old female patient, a regular user of soft contact lenses without good practices of lens hygiene and handli...

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Autores principales: Gertiser, María Laura, Giagante, E., Sgattoni, E., Basabe, N., Rivero, Fernando David, Luján, Hugo Daniel, Occhionero, M., Paniccia, L., Visciarelli, E., Costamagna, S.R.
Formato: Artículo NonPeerReviewed
Lenguaje:Español
Publicado: 2010
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Acceso en línea:http://pa.bibdigital.ucc.edu.ar/4994/1/A_Gertiser.pdf
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id I38-R144-4994
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spelling I38-R144-49942025-11-06T15:12:42Z http://pa.bibdigital.ucc.edu.ar/4994/ Acanthamoeba sp. keratitis: First case confirmed by isolation and molecular typification in Bahía Blanca, Buenos Aires Province, Argentina [Queratitis por Acanthamoeba sp.: Primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina] Gertiser, María Laura Giagante, E. Sgattoni, E. Basabe, N. Rivero, Fernando David Luján, Hugo Daniel Occhionero, M. Paniccia, L. Visciarelli, E. Costamagna, S.R. R Medicina (General) Some species of the Acanthamoeba genus cause keratitis, a very painful, most likely unilateral corneal infection, associated with eye and vision impairment. We here present a case of a 31-year-old female patient, a regular user of soft contact lenses without good practices of lens hygiene and handling. The patient attended medical consultation after two months of inflammation and pain in her right eye. After ophthalmological studies, and due to suspicion of a parasitic infection, a biopsy was performed and the sample submitted for bacteriological and parasitological analyses. Moreover, contact lens holders and lens cleaning solutions were studied. The samples yielded negative results for bacterial infection. However, cultivation of all samples showed the presence of amoeboid parasites. Isolated amoebae were morphologically and molecularly classified as members of the Acanthamoeba genus. This is the first case of keratitis caused by Acanthamoeba in Bahía Blanca, Buenos Aires Province, where the parasite was identified by specific and sensitive molecular techniques. 2010-12-31 Artículo NonPeerReviewed application/pdf spa http://pa.bibdigital.ucc.edu.ar/4994/1/A_Gertiser.pdf Gertiser, María Laura ORCID: https://orcid.org/0000-0001-6820-8690 <https://orcid.org/0000-0001-6820-8690>, Giagante, E., Sgattoni, E., Basabe, N., Rivero, Fernando David, Luján, Hugo Daniel ORCID: https://orcid.org/0000-0002-3797-8315 <https://orcid.org/0000-0002-3797-8315>, Occhionero, M., Paniccia, L., Visciarelli, E. and Costamagna, S.R. (2010) Acanthamoeba sp. keratitis: First case confirmed by isolation and molecular typification in Bahía Blanca, Buenos Aires Province, Argentina [Queratitis por Acanthamoeba sp.: Primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina]. Revista Argentina de Microbiologia, 42 (2). pp. 122-125. ISSN 03257541
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic R Medicina (General)
spellingShingle R Medicina (General)
Gertiser, María Laura
Giagante, E.
Sgattoni, E.
Basabe, N.
Rivero, Fernando David
Luján, Hugo Daniel
Occhionero, M.
Paniccia, L.
Visciarelli, E.
Costamagna, S.R.
Acanthamoeba sp. keratitis: First case confirmed by isolation and molecular typification in Bahía Blanca, Buenos Aires Province, Argentina [Queratitis por Acanthamoeba sp.: Primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina]
topic_facet R Medicina (General)
description Some species of the Acanthamoeba genus cause keratitis, a very painful, most likely unilateral corneal infection, associated with eye and vision impairment. We here present a case of a 31-year-old female patient, a regular user of soft contact lenses without good practices of lens hygiene and handling. The patient attended medical consultation after two months of inflammation and pain in her right eye. After ophthalmological studies, and due to suspicion of a parasitic infection, a biopsy was performed and the sample submitted for bacteriological and parasitological analyses. Moreover, contact lens holders and lens cleaning solutions were studied. The samples yielded negative results for bacterial infection. However, cultivation of all samples showed the presence of amoeboid parasites. Isolated amoebae were morphologically and molecularly classified as members of the Acanthamoeba genus. This is the first case of keratitis caused by Acanthamoeba in Bahía Blanca, Buenos Aires Province, where the parasite was identified by specific and sensitive molecular techniques.
format Artículo
NonPeerReviewed
author Gertiser, María Laura
Giagante, E.
Sgattoni, E.
Basabe, N.
Rivero, Fernando David
Luján, Hugo Daniel
Occhionero, M.
Paniccia, L.
Visciarelli, E.
Costamagna, S.R.
author_facet Gertiser, María Laura
Giagante, E.
Sgattoni, E.
Basabe, N.
Rivero, Fernando David
Luján, Hugo Daniel
Occhionero, M.
Paniccia, L.
Visciarelli, E.
Costamagna, S.R.
author_sort Gertiser, María Laura
title Acanthamoeba sp. keratitis: First case confirmed by isolation and molecular typification in Bahía Blanca, Buenos Aires Province, Argentina [Queratitis por Acanthamoeba sp.: Primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina]
title_short Acanthamoeba sp. keratitis: First case confirmed by isolation and molecular typification in Bahía Blanca, Buenos Aires Province, Argentina [Queratitis por Acanthamoeba sp.: Primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina]
title_full Acanthamoeba sp. keratitis: First case confirmed by isolation and molecular typification in Bahía Blanca, Buenos Aires Province, Argentina [Queratitis por Acanthamoeba sp.: Primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina]
title_fullStr Acanthamoeba sp. keratitis: First case confirmed by isolation and molecular typification in Bahía Blanca, Buenos Aires Province, Argentina [Queratitis por Acanthamoeba sp.: Primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina]
title_full_unstemmed Acanthamoeba sp. keratitis: First case confirmed by isolation and molecular typification in Bahía Blanca, Buenos Aires Province, Argentina [Queratitis por Acanthamoeba sp.: Primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina]
title_sort acanthamoeba sp. keratitis: first case confirmed by isolation and molecular typification in bahía blanca, buenos aires province, argentina [queratitis por acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en bahía blanca, provincia de buenos aires, argentina]
publishDate 2010
url http://pa.bibdigital.ucc.edu.ar/4994/1/A_Gertiser.pdf
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