Evidence of bacterial Biofilms in nasal polyposis
Introduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have been implicated in many infl ammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas, and more recently CRS/NP. We propose that the bac...
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| Autores principales: | , , , , , , , |
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| Formato: | Artículo PeerReviewed |
| Lenguaje: | Español |
| Publicado: |
2010
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| Materias: | |
| Acceso en línea: | http://pa.bibdigital.ucc.edu.ar/4966/1/A_Zernotti.pdf |
| Aporte de: |
| Sumario: | Introduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have been implicated in many infl ammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas, and more recently CRS/NP. We propose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities as Biofilms. Objective: To determine and characterize the presence of Biofilms in CRS/NP. Methods: We performed a prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. Ten patients without CRS/NP who underwent septoplasty were included as a control group. Tissue samples were obtained from the inferior turbinate mucosae. The bacteria were isolated and typifi ed and the material was examined in vitro using a spectrophotometer, and in vivo using optical microscopy and confocal scanning laser microscopy. Results: Moderate to high in vitro Biofilm-forming capacity was detected in 9 out of 12 patients with CRS/NP (mean [SD] optical density values of between 0.284 [0.017] and 3.337 [0.029]). The microorganisms isolated were Staphylococcus (5 patients), Streptococcus viridans, Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corynebacterium. Biofilms were demonstrated in vivo in 2 patients and no Biofilm structures were evident in any of the controls. Conclusion: This study demonstrates the presence of bacterial Biofilms in patients with CRS/NP. This chronic infl ammatory factor might contribute to nasal mucosa damage, increased infl ammatory cells in tissue, and the subsequent hyperplasic process. |
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