Evidence of bacterial Biofilms in nasal polyposis

Introduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have been implicated in many infl ammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas, and more recently CRS/NP. We propose that the bac...

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Autores principales: Zernotti, Mario Emilio, Villegas, Ángel, Revol, Roque, Baena Cagnani, Carlos E., Miranda Arce, J.E., Paredes, M.E., Albesa, I., Paraje, María Gabriela
Formato: Artículo PeerReviewed
Lenguaje:Español
Publicado: 2010
Materias:
R Medicina (General)
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/4966/1/A_Zernotti.pdf
Aporte de:
Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-4966
record_format dspace
spelling I38-R144-49662025-10-30T12:50:29Z http://pa.bibdigital.ucc.edu.ar/4966/ Evidence of bacterial Biofilms in nasal polyposis Zernotti, Mario Emilio Villegas, Ángel Revol, Roque Baena Cagnani, Carlos E. Miranda Arce, J.E. Paredes, M.E. Albesa, I. Paraje, María Gabriela R Medicina (General) Introduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have been implicated in many infl ammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas, and more recently CRS/NP. We propose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities as Biofilms. Objective: To determine and characterize the presence of Biofilms in CRS/NP. Methods: We performed a prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. Ten patients without CRS/NP who underwent septoplasty were included as a control group. Tissue samples were obtained from the inferior turbinate mucosae. The bacteria were isolated and typifi ed and the material was examined in vitro using a spectrophotometer, and in vivo using optical microscopy and confocal scanning laser microscopy. Results: Moderate to high in vitro Biofilm-forming capacity was detected in 9 out of 12 patients with CRS/NP (mean [SD] optical density values of between 0.284 [0.017] and 3.337 [0.029]). The microorganisms isolated were Staphylococcus (5 patients), Streptococcus viridans, Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corynebacterium. Biofilms were demonstrated in vivo in 2 patients and no Biofilm structures were evident in any of the controls. Conclusion: This study demonstrates the presence of bacterial Biofilms in patients with CRS/NP. This chronic infl ammatory factor might contribute to nasal mucosa damage, increased infl ammatory cells in tissue, and the subsequent hyperplasic process. 2010-12-31 Artículo PeerReviewed application/pdf spa http://pa.bibdigital.ucc.edu.ar/4966/1/A_Zernotti.pdf Zernotti, Mario Emilio ORCID: https://orcid.org/0000-0003-4288-2809 <https://orcid.org/0000-0003-4288-2809>, Villegas, Ángel, Revol, Roque, Baena Cagnani, Carlos E., Miranda Arce, J.E., Paredes, M.E., Albesa, I. and Paraje, María Gabriela ORCID: https://orcid.org/0000-0001-8600-1159 <https://orcid.org/0000-0001-8600-1159> (2010) Evidence of bacterial Biofilms in nasal polyposis. Evidence of bacterial Biofilms in nasal polyposis, 20 (5). pp. 380-385. ISSN 20945603
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic R Medicina (General)
spellingShingle R Medicina (General)
Zernotti, Mario Emilio
Villegas, Ángel
Revol, Roque
Baena Cagnani, Carlos E.
Miranda Arce, J.E.
Paredes, M.E.
Albesa, I.
Paraje, María Gabriela
Evidence of bacterial Biofilms in nasal polyposis
topic_facet R Medicina (General)
description Introduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have been implicated in many infl ammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas, and more recently CRS/NP. We propose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities as Biofilms. Objective: To determine and characterize the presence of Biofilms in CRS/NP. Methods: We performed a prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. Ten patients without CRS/NP who underwent septoplasty were included as a control group. Tissue samples were obtained from the inferior turbinate mucosae. The bacteria were isolated and typifi ed and the material was examined in vitro using a spectrophotometer, and in vivo using optical microscopy and confocal scanning laser microscopy. Results: Moderate to high in vitro Biofilm-forming capacity was detected in 9 out of 12 patients with CRS/NP (mean [SD] optical density values of between 0.284 [0.017] and 3.337 [0.029]). The microorganisms isolated were Staphylococcus (5 patients), Streptococcus viridans, Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corynebacterium. Biofilms were demonstrated in vivo in 2 patients and no Biofilm structures were evident in any of the controls. Conclusion: This study demonstrates the presence of bacterial Biofilms in patients with CRS/NP. This chronic infl ammatory factor might contribute to nasal mucosa damage, increased infl ammatory cells in tissue, and the subsequent hyperplasic process.
format Artículo
PeerReviewed
author Zernotti, Mario Emilio
Villegas, Ángel
Revol, Roque
Baena Cagnani, Carlos E.
Miranda Arce, J.E.
Paredes, M.E.
Albesa, I.
Paraje, María Gabriela
author_facet Zernotti, Mario Emilio
Villegas, Ángel
Revol, Roque
Baena Cagnani, Carlos E.
Miranda Arce, J.E.
Paredes, M.E.
Albesa, I.
Paraje, María Gabriela
author_sort Zernotti, Mario Emilio
title Evidence of bacterial Biofilms in nasal polyposis
title_short Evidence of bacterial Biofilms in nasal polyposis
title_full Evidence of bacterial Biofilms in nasal polyposis
title_fullStr Evidence of bacterial Biofilms in nasal polyposis
title_full_unstemmed Evidence of bacterial Biofilms in nasal polyposis
title_sort evidence of bacterial biofilms in nasal polyposis
publishDate 2010
url http://pa.bibdigital.ucc.edu.ar/4966/1/A_Zernotti.pdf
work_keys_str_mv AT zernottimarioemilio evidenceofbacterialbiofilmsinnasalpolyposis
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AT mirandaarceje evidenceofbacterialbiofilmsinnasalpolyposis
AT paredesme evidenceofbacterialbiofilmsinnasalpolyposis
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