A simple allele-specific polymerase chain reaction method to detect the Gly143Glu polymorphism in the human carboxylesterase 1 gene: importance of genotyping for pharmacogenetic treatment

Human carboxylesterases 1 and 2 (CES1 and CES2) catalyze the hydrolysis of many exogenous compounds. Alterations in CES sequences could lead to variability in both the inactivation of drugs and the activation of prodrugs. The human CES1 gene encodes for the enzyme carboxylesterase 1, a serine estera...

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Autores principales: Soria, Néstor Walter, Belaus, Andrea, Galván, Cristian Ariel, Pasquali, María Ana, Vélez, Pablo, Montes, Cecilia del Carmen, Beltramo, Dante Miguel
Formato: Artículo PeerReviewed
Lenguaje:Español
Publicado: 2010
Materias:
QD Química
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/4965/1/A_Soria.pdf
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Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-4965
record_format dspace
spelling I38-R144-49652025-10-30T12:43:23Z http://pa.bibdigital.ucc.edu.ar/4965/ A simple allele-specific polymerase chain reaction method to detect the Gly143Glu polymorphism in the human carboxylesterase 1 gene: importance of genotyping for pharmacogenetic treatment Soria, Néstor Walter Belaus, Andrea Galván, Cristian Ariel Pasquali, María Ana Vélez, Pablo Montes, Cecilia del Carmen Beltramo, Dante Miguel QD Química Human carboxylesterases 1 and 2 (CES1 and CES2) catalyze the hydrolysis of many exogenous compounds. Alterations in CES sequences could lead to variability in both the inactivation of drugs and the activation of prodrugs. The human CES1 gene encodes for the enzyme carboxylesterase 1, a serine esterase governing both metabolic deactivation and activation of numerous therapeutic agents. Some of theses drugs are the antiviral oseltamivir used to treat some types of influenza infections and the methylphenidate employed in the treatment of patients with attention deficit. The Gly143Glu polymorphism in CES1 gene has been shown to reduce enzyme activity. The aim of the present study was to develop an easy and cheap method to detect this polymorphism. For this, we studied a group of people from Córdoba, a Mediterranean area from Argentina. Our results show that our methodology could detect the presence of this polymorphism with a frequency around 1.8%, only in the heterozygote form. These results could be relevant to patients before the treatment with some drugs where the CES1 enzyme is involved. 2010-12-31 Artículo PeerReviewed application/pdf spa http://pa.bibdigital.ucc.edu.ar/4965/1/A_Soria.pdf Soria, Néstor Walter ORCID: https://orcid.org/0000-0002-8232-1822 <https://orcid.org/0000-0002-8232-1822>, Belaus, Andrea, Galván, Cristian Ariel ORCID: https://orcid.org/0000-0002-1885-3664 <https://orcid.org/0000-0002-1885-3664>, Pasquali, María Ana, Vélez, Pablo, Montes, Cecilia del Carmen and Beltramo, Dante Miguel ORCID: https://orcid.org/0000-0002-8198-3525 <https://orcid.org/0000-0002-8198-3525> (2010) A simple allele-specific polymerase chain reaction method to detect the Gly143Glu polymorphism in the human carboxylesterase 1 gene: importance of genotyping for pharmacogenetic treatment. Genetic Testing and Molecular Biomarkers, 14 (6). pp. 749-751. ISSN 19450257 info:eu-repo/semantics/altIdentifier/doi/10.1089/gtmb.2010.0037
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic QD Química
spellingShingle QD Química
Soria, Néstor Walter
Belaus, Andrea
Galván, Cristian Ariel
Pasquali, María Ana
Vélez, Pablo
Montes, Cecilia del Carmen
Beltramo, Dante Miguel
A simple allele-specific polymerase chain reaction method to detect the Gly143Glu polymorphism in the human carboxylesterase 1 gene: importance of genotyping for pharmacogenetic treatment
topic_facet QD Química
description Human carboxylesterases 1 and 2 (CES1 and CES2) catalyze the hydrolysis of many exogenous compounds. Alterations in CES sequences could lead to variability in both the inactivation of drugs and the activation of prodrugs. The human CES1 gene encodes for the enzyme carboxylesterase 1, a serine esterase governing both metabolic deactivation and activation of numerous therapeutic agents. Some of theses drugs are the antiviral oseltamivir used to treat some types of influenza infections and the methylphenidate employed in the treatment of patients with attention deficit. The Gly143Glu polymorphism in CES1 gene has been shown to reduce enzyme activity. The aim of the present study was to develop an easy and cheap method to detect this polymorphism. For this, we studied a group of people from Córdoba, a Mediterranean area from Argentina. Our results show that our methodology could detect the presence of this polymorphism with a frequency around 1.8%, only in the heterozygote form. These results could be relevant to patients before the treatment with some drugs where the CES1 enzyme is involved.
format Artículo
PeerReviewed
author Soria, Néstor Walter
Belaus, Andrea
Galván, Cristian Ariel
Pasquali, María Ana
Vélez, Pablo
Montes, Cecilia del Carmen
Beltramo, Dante Miguel
author_facet Soria, Néstor Walter
Belaus, Andrea
Galván, Cristian Ariel
Pasquali, María Ana
Vélez, Pablo
Montes, Cecilia del Carmen
Beltramo, Dante Miguel
author_sort Soria, Néstor Walter
title A simple allele-specific polymerase chain reaction method to detect the Gly143Glu polymorphism in the human carboxylesterase 1 gene: importance of genotyping for pharmacogenetic treatment
title_short A simple allele-specific polymerase chain reaction method to detect the Gly143Glu polymorphism in the human carboxylesterase 1 gene: importance of genotyping for pharmacogenetic treatment
title_full A simple allele-specific polymerase chain reaction method to detect the Gly143Glu polymorphism in the human carboxylesterase 1 gene: importance of genotyping for pharmacogenetic treatment
title_fullStr A simple allele-specific polymerase chain reaction method to detect the Gly143Glu polymorphism in the human carboxylesterase 1 gene: importance of genotyping for pharmacogenetic treatment
title_full_unstemmed A simple allele-specific polymerase chain reaction method to detect the Gly143Glu polymorphism in the human carboxylesterase 1 gene: importance of genotyping for pharmacogenetic treatment
title_sort simple allele-specific polymerase chain reaction method to detect the gly143glu polymorphism in the human carboxylesterase 1 gene: importance of genotyping for pharmacogenetic treatment
publishDate 2010
url http://pa.bibdigital.ucc.edu.ar/4965/1/A_Soria.pdf
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