ORF-C4 from the early branching eukaryote giardia lamblia displays characteristics of α-crystallin small heat-shock proteins

Giardia lamblia is a medically important protozoan parasite with a basal position in the eukaryotic lineage and is an interesting model to explain the evolution of biochemical events in eukaryotic cells. G. lamblia trophozoites undergo significant changes in order to survive outside the intestine of...

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Autores principales: Norés, María Jimena, Prucca, César Germán, Quiroga, Rodrigo, Elías, Eliana Vanina, Cavallín, Lucas, Price, Argenta M., Saura, Alicia, Carranza, Pedro Gabriel, Gottig, Natalia, Solari, Alberto J., Luján, Hugo Daniel
Formato: Artículo PeerReviewed
Lenguaje:Español
Publicado: 2009
Materias:
R Medicina (General)
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/4950/1/A_Nores.pdf
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Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-4950
record_format dspace
spelling I38-R144-49502025-10-24T16:29:57Z http://pa.bibdigital.ucc.edu.ar/4950/ ORF-C4 from the early branching eukaryote giardia lamblia displays characteristics of α-crystallin small heat-shock proteins Norés, María Jimena Prucca, César Germán Quiroga, Rodrigo Elías, Eliana Vanina Cavallín, Lucas Price, Argenta M. Saura, Alicia Carranza, Pedro Gabriel Gottig, Natalia Solari, Alberto J. Luján, Hugo Daniel R Medicina (General) Giardia lamblia is a medically important protozoan parasite with a basal position in the eukaryotic lineage and is an interesting model to explain the evolution of biochemical events in eukaryotic cells. G. lamblia trophozoites undergo significant changes in order to survive outside the intestine of their host by differentiating into infective cysts. In the present study, we characterize the previously identified Orf-C4 (G. lamblia open reading frame C4) gene, which is considered to be specific to G. lamblia. It encodes a 22 kDa protein that assembles into high-molecularmass complexes during the entire life cycle of the parasite. ORF-C4 localizes to the cytoplasm of trophozoites and cysts, and forms large spherical aggregates when overexpressed. ORF-C4 overexpression and down-regulation do not affect trophozoite viability; however, differentiation into cysts is slightly delayed when the expression of ORFC4 is down-regulated. In addition, ORF-C4 protein expression is modified under specific stress-inducing conditions. Neither orthologous proteins nor conserved domains are found in databases by conventional sequence analysis of the predicted protein. However, ORF-C4 contains a region which is similar structurally to the a-crystallin domain of sHsps (small heat-shock proteins). In the present study, we show the potential role of ORF-C4 as a small chaperone which is involved in the response to stress (including encystation) in G. lamblia. 2009-12-31 Artículo PeerReviewed application/pdf spa http://pa.bibdigital.ucc.edu.ar/4950/1/A_Nores.pdf Norés, María Jimena ORCID: https://orcid.org/0000-0003-1628-6069 <https://orcid.org/0000-0003-1628-6069>, Prucca, César Germán ORCID: https://orcid.org/0000-0003-4165-4660 <https://orcid.org/0000-0003-4165-4660>, Quiroga, Rodrigo ORCID: https://orcid.org/0000-0001-5015-0531 <https://orcid.org/0000-0001-5015-0531>, Elías, Eliana Vanina ORCID: https://orcid.org/0000-0003-2516-9219 <https://orcid.org/0000-0003-2516-9219>, Cavallín, Lucas, Price, Argenta M. ORCID: https://orcid.org/0000-0002-3692-1683 <https://orcid.org/0000-0002-3692-1683>, Saura, Alicia ORCID: https://orcid.org/0000-0003-1537-506X <https://orcid.org/0000-0003-1537-506X>, Carranza, Pedro Gabriel ORCID: https://orcid.org/0000-0002-1145-3852 <https://orcid.org/0000-0002-1145-3852>, Gottig, Natalia ORCID: https://orcid.org/0000-0002-8730-8248 <https://orcid.org/0000-0002-8730-8248>, Solari, Alberto J. and Luján, Hugo Daniel ORCID: https://orcid.org/0000-0002-3797-8315 <https://orcid.org/0000-0002-3797-8315> (2009) ORF-C4 from the early branching eukaryote giardia lamblia displays characteristics of α-crystallin small heat-shock proteins. Bioscience Reports, 29 (1). pp. 25-34. ISSN 18680481 info:eu-repo/semantics/altIdentifier/doi/10.1042/BSR20080101
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic R Medicina (General)
spellingShingle R Medicina (General)
Norés, María Jimena
Prucca, César Germán
Quiroga, Rodrigo
Elías, Eliana Vanina
Cavallín, Lucas
Price, Argenta M.
Saura, Alicia
Carranza, Pedro Gabriel
Gottig, Natalia
Solari, Alberto J.
Luján, Hugo Daniel
ORF-C4 from the early branching eukaryote giardia lamblia displays characteristics of α-crystallin small heat-shock proteins
topic_facet R Medicina (General)
description Giardia lamblia is a medically important protozoan parasite with a basal position in the eukaryotic lineage and is an interesting model to explain the evolution of biochemical events in eukaryotic cells. G. lamblia trophozoites undergo significant changes in order to survive outside the intestine of their host by differentiating into infective cysts. In the present study, we characterize the previously identified Orf-C4 (G. lamblia open reading frame C4) gene, which is considered to be specific to G. lamblia. It encodes a 22 kDa protein that assembles into high-molecularmass complexes during the entire life cycle of the parasite. ORF-C4 localizes to the cytoplasm of trophozoites and cysts, and forms large spherical aggregates when overexpressed. ORF-C4 overexpression and down-regulation do not affect trophozoite viability; however, differentiation into cysts is slightly delayed when the expression of ORFC4 is down-regulated. In addition, ORF-C4 protein expression is modified under specific stress-inducing conditions. Neither orthologous proteins nor conserved domains are found in databases by conventional sequence analysis of the predicted protein. However, ORF-C4 contains a region which is similar structurally to the a-crystallin domain of sHsps (small heat-shock proteins). In the present study, we show the potential role of ORF-C4 as a small chaperone which is involved in the response to stress (including encystation) in G. lamblia.
format Artículo
PeerReviewed
author Norés, María Jimena
Prucca, César Germán
Quiroga, Rodrigo
Elías, Eliana Vanina
Cavallín, Lucas
Price, Argenta M.
Saura, Alicia
Carranza, Pedro Gabriel
Gottig, Natalia
Solari, Alberto J.
Luján, Hugo Daniel
author_facet Norés, María Jimena
Prucca, César Germán
Quiroga, Rodrigo
Elías, Eliana Vanina
Cavallín, Lucas
Price, Argenta M.
Saura, Alicia
Carranza, Pedro Gabriel
Gottig, Natalia
Solari, Alberto J.
Luján, Hugo Daniel
author_sort Norés, María Jimena
title ORF-C4 from the early branching eukaryote giardia lamblia displays characteristics of α-crystallin small heat-shock proteins
title_short ORF-C4 from the early branching eukaryote giardia lamblia displays characteristics of α-crystallin small heat-shock proteins
title_full ORF-C4 from the early branching eukaryote giardia lamblia displays characteristics of α-crystallin small heat-shock proteins
title_fullStr ORF-C4 from the early branching eukaryote giardia lamblia displays characteristics of α-crystallin small heat-shock proteins
title_full_unstemmed ORF-C4 from the early branching eukaryote giardia lamblia displays characteristics of α-crystallin small heat-shock proteins
title_sort orf-c4 from the early branching eukaryote giardia lamblia displays characteristics of α-crystallin small heat-shock proteins
publishDate 2009
url http://pa.bibdigital.ucc.edu.ar/4950/1/A_Nores.pdf
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