Use of cholinesterase activity in monitoring chlorpyrifos exposure of steer cattle after topical administration

The aim of this work was to study the pharmacokinetic behavior and the inhibitory effect of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities of chlorpyrifos (CPF) in steer cattle after pour-on administration. Determination of cholinesterase activity in plasma and erythrocyte w...

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Autores principales: Picco, Eduardo J., Fernández, Héctor R., Díaz David, Diego C., San Andrés, Manuel I., Boggio, Juan Carlos, Rodriguez Fenández, Casilda
Formato: Artículo
Lenguaje:Español
Publicado: 2008
Materias:
SB Cultura de la planta
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/4815/1/A_Picco.pdf
Aporte de:
Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-4815
record_format dspace
spelling I38-R144-48152025-05-07T18:08:30Z http://pa.bibdigital.ucc.edu.ar/4815/ Use of cholinesterase activity in monitoring chlorpyrifos exposure of steer cattle after topical administration Picco, Eduardo J. Fernández, Héctor R. Díaz David, Diego C. San Andrés, Manuel I. Boggio, Juan Carlos Rodriguez Fenández, Casilda SB Cultura de la planta The aim of this work was to study the pharmacokinetic behavior and the inhibitory effect of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities of chlorpyrifos (CPF) in steer cattle after pour-on administration. Determination of cholinesterase activity in plasma and erythrocyte was carried out according to Ellman kinetic method. CPF was analyzed by gas chromatography. AChE was the predominant form of cholinesterase analyzed, with low levels of BChE in plasma. Following the treatment with CPF, the maximum inhibitory effect on AChE or BChE were 50.88 ± 11.57 and 42.66 ± 12.01%, respectively. The chlorpyrifos plasma concentrations observed were low and they presented a high variability. Chlorpyrifos peak plasma concentration (10.42 ± 4.76 μ g/L) was reached at 8.42 ± 13.97 h. The pesticide was not detected in plasma after 48 h post treatment. The values of area under the curve (AUC) were 118.48 ± 87.46 μ g· h/L and mean resistance time (MRT) were 13.38 ± 10.41 h. The pour-on exposure to the organophosphate chlorpyrifos significantly reduced AChE and BChE activity in steer cattle and the recovery was not reached on 50 days post-treatment. 2008-12-31 info:eu-repo/semantics/article info:eu-repo/semantics/closedAccess application/pdf spa http://pa.bibdigital.ucc.edu.ar/4815/1/A_Picco.pdf Picco, Eduardo J., Fernández, Héctor R., Díaz David, Diego C., San Andrés, Manuel I., Boggio, Juan Carlos ORCID: https://orcid.org/0000-0001-9748-001X <https://orcid.org/0000-0001-9748-001X> and Rodriguez Fenández, Casilda (2008) Use of cholinesterase activity in monitoring chlorpyrifos exposure of steer cattle after topical administration. Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes, 43 (5). pp. 405-409. ISSN 15324109 info:eu-repo/semantics/altIdentifier/doi/10.1080/03601230802062158
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic SB Cultura de la planta
spellingShingle SB Cultura de la planta
Picco, Eduardo J.
Fernández, Héctor R.
Díaz David, Diego C.
San Andrés, Manuel I.
Boggio, Juan Carlos
Rodriguez Fenández, Casilda
Use of cholinesterase activity in monitoring chlorpyrifos exposure of steer cattle after topical administration
topic_facet SB Cultura de la planta
description The aim of this work was to study the pharmacokinetic behavior and the inhibitory effect of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities of chlorpyrifos (CPF) in steer cattle after pour-on administration. Determination of cholinesterase activity in plasma and erythrocyte was carried out according to Ellman kinetic method. CPF was analyzed by gas chromatography. AChE was the predominant form of cholinesterase analyzed, with low levels of BChE in plasma. Following the treatment with CPF, the maximum inhibitory effect on AChE or BChE were 50.88 ± 11.57 and 42.66 ± 12.01%, respectively. The chlorpyrifos plasma concentrations observed were low and they presented a high variability. Chlorpyrifos peak plasma concentration (10.42 ± 4.76 μ g/L) was reached at 8.42 ± 13.97 h. The pesticide was not detected in plasma after 48 h post treatment. The values of area under the curve (AUC) were 118.48 ± 87.46 μ g· h/L and mean resistance time (MRT) were 13.38 ± 10.41 h. The pour-on exposure to the organophosphate chlorpyrifos significantly reduced AChE and BChE activity in steer cattle and the recovery was not reached on 50 days post-treatment.
format Artículo
author Picco, Eduardo J.
Fernández, Héctor R.
Díaz David, Diego C.
San Andrés, Manuel I.
Boggio, Juan Carlos
Rodriguez Fenández, Casilda
author_facet Picco, Eduardo J.
Fernández, Héctor R.
Díaz David, Diego C.
San Andrés, Manuel I.
Boggio, Juan Carlos
Rodriguez Fenández, Casilda
author_sort Picco, Eduardo J.
title Use of cholinesterase activity in monitoring chlorpyrifos exposure of steer cattle after topical administration
title_short Use of cholinesterase activity in monitoring chlorpyrifos exposure of steer cattle after topical administration
title_full Use of cholinesterase activity in monitoring chlorpyrifos exposure of steer cattle after topical administration
title_fullStr Use of cholinesterase activity in monitoring chlorpyrifos exposure of steer cattle after topical administration
title_full_unstemmed Use of cholinesterase activity in monitoring chlorpyrifos exposure of steer cattle after topical administration
title_sort use of cholinesterase activity in monitoring chlorpyrifos exposure of steer cattle after topical administration
publishDate 2008
url http://pa.bibdigital.ucc.edu.ar/4815/1/A_Picco.pdf
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