Antigenic variation in Giardia lamblia is regulated by RNA interference

Giardia lamblia (also called Giardia intestinalis) is one of the most common intestinal parasites of humans. To evade the host's immune response, Giardia undergoes antigenic variation - a process that allows the parasite to develop chronic and recurrent infections. From a repertoire of ∼190 var...

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Autores principales: Prucca, César Germán, Slavin, Ileana, Quiroga, Rodrigo, Elías, Eliana Vanina, Rivero, Fernando David, Saura, Alicia, Carranza, Pedro Gabriel, Luján, Hugo Daniel
Formato: Artículo
Lenguaje:Español
Publicado: 2008
Materias:
R Medicina (General)
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/4045/1/A_Prucca....pdf
Aporte de:
Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-4045
record_format dspace
spelling I38-R144-40452025-10-24T16:33:55Z http://pa.bibdigital.ucc.edu.ar/4045/ Antigenic variation in Giardia lamblia is regulated by RNA interference Prucca, César Germán Slavin, Ileana Quiroga, Rodrigo Elías, Eliana Vanina Rivero, Fernando David Saura, Alicia Carranza, Pedro Gabriel Luján, Hugo Daniel R Medicina (General) Giardia lamblia (also called Giardia intestinalis) is one of the most common intestinal parasites of humans. To evade the host's immune response, Giardia undergoes antigenic variation - a process that allows the parasite to develop chronic and recurrent infections. From a repertoire of ∼190 variant-specific surface protein (VSP)-coding genes, Giardia expresses only one VSP on the surface of each parasite at a particular time, but spontaneously switches to a different VSP by unknown mechanisms. Here we show that regulation of VSP expression involves a system comprising RNA-dependent RNA polymerase, Dicer and Argonaute, known components of the RNA interference machinery. Clones expressing a single surface antigen efficiently transcribe several VSP genes but only accumulate transcripts encoding the VSP to be expressed. Detection of antisense RNAs corresponding to the silenced VSP genes and small RNAs from the silenced but not for the expressed vsp implicate the RNA interference pathway in antigenic variation. Remarkably, silencing of Dicer and RNA-dependent RNA polymerase leads to a change from single to multiple VSP expression in individual parasites. 2008-12-11 info:eu-repo/semantics/article info:eu-repo/semantics/closedAccess application/pdf spa http://pa.bibdigital.ucc.edu.ar/4045/1/A_Prucca....pdf Prucca, César Germán ORCID: https://orcid.org/0000-0003-4165-4660 <https://orcid.org/0000-0003-4165-4660>, Slavin, Ileana, Quiroga, Rodrigo ORCID: https://orcid.org/0000-0001-5015-0531 <https://orcid.org/0000-0001-5015-0531>, Elías, Eliana Vanina ORCID: https://orcid.org/0000-0003-2516-9219 <https://orcid.org/0000-0003-2516-9219>, Rivero, Fernando David, Saura, Alicia ORCID: https://orcid.org/0000-0003-1537-506X <https://orcid.org/0000-0003-1537-506X>, Carranza, Pedro Gabriel ORCID: https://orcid.org/0000-0002-1145-3852 <https://orcid.org/0000-0002-1145-3852> and Luján, Hugo Daniel ORCID: https://orcid.org/0000-0002-3797-8315 <https://orcid.org/0000-0002-3797-8315> (2008) Antigenic variation in Giardia lamblia is regulated by RNA interference. Nature, 456 (7223). pp. 750-754. ISSN 0028-0836 info:eu-repo/semantics/altIdentifier/doi/10.1038/nature07585
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic R Medicina (General)
spellingShingle R Medicina (General)
Prucca, César Germán
Slavin, Ileana
Quiroga, Rodrigo
Elías, Eliana Vanina
Rivero, Fernando David
Saura, Alicia
Carranza, Pedro Gabriel
Luján, Hugo Daniel
Antigenic variation in Giardia lamblia is regulated by RNA interference
topic_facet R Medicina (General)
description Giardia lamblia (also called Giardia intestinalis) is one of the most common intestinal parasites of humans. To evade the host's immune response, Giardia undergoes antigenic variation - a process that allows the parasite to develop chronic and recurrent infections. From a repertoire of ∼190 variant-specific surface protein (VSP)-coding genes, Giardia expresses only one VSP on the surface of each parasite at a particular time, but spontaneously switches to a different VSP by unknown mechanisms. Here we show that regulation of VSP expression involves a system comprising RNA-dependent RNA polymerase, Dicer and Argonaute, known components of the RNA interference machinery. Clones expressing a single surface antigen efficiently transcribe several VSP genes but only accumulate transcripts encoding the VSP to be expressed. Detection of antisense RNAs corresponding to the silenced VSP genes and small RNAs from the silenced but not for the expressed vsp implicate the RNA interference pathway in antigenic variation. Remarkably, silencing of Dicer and RNA-dependent RNA polymerase leads to a change from single to multiple VSP expression in individual parasites.
format Artículo
author Prucca, César Germán
Slavin, Ileana
Quiroga, Rodrigo
Elías, Eliana Vanina
Rivero, Fernando David
Saura, Alicia
Carranza, Pedro Gabriel
Luján, Hugo Daniel
author_facet Prucca, César Germán
Slavin, Ileana
Quiroga, Rodrigo
Elías, Eliana Vanina
Rivero, Fernando David
Saura, Alicia
Carranza, Pedro Gabriel
Luján, Hugo Daniel
author_sort Prucca, César Germán
title Antigenic variation in Giardia lamblia is regulated by RNA interference
title_short Antigenic variation in Giardia lamblia is regulated by RNA interference
title_full Antigenic variation in Giardia lamblia is regulated by RNA interference
title_fullStr Antigenic variation in Giardia lamblia is regulated by RNA interference
title_full_unstemmed Antigenic variation in Giardia lamblia is regulated by RNA interference
title_sort antigenic variation in giardia lamblia is regulated by rna interference
publishDate 2008
url http://pa.bibdigital.ucc.edu.ar/4045/1/A_Prucca....pdf
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