Characterization of SNAREs determines the absence of a typical Golgi apparatus in the ancient eukaryote Giardia lamblia

Giardia is a eukaryotic protozoal parasite with unusual characteristics, such as the absence of a morphologically evident Golgi apparatus. Although both constitutive and regulated pathways for protein secretion are evident in Giardia, little is known about the mechanisms involved in vesicular dockin...

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Autores principales: Elías, Eliana Vanina, Quiroga, Rodrigo, Gottig, Natalia, Nakanishi, Hideki, Nash, Theodore, Neiman, Aaron, Luján, Hugo Daniel
Formato: Artículo acceptedVersion
Lenguaje:Español
Publicado: 2008
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R Medicina (General)
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/4044/1/A_Elias_Quiroga_Gottig_Nakanishi_Nash_Neiman_Lujan.pdf
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Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-4044
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spelling I38-R144-40442023-08-23T18:06:18Z http://pa.bibdigital.ucc.edu.ar/4044/ Characterization of SNAREs determines the absence of a typical Golgi apparatus in the ancient eukaryote Giardia lamblia Elías, Eliana Vanina Quiroga, Rodrigo Gottig, Natalia Nakanishi, Hideki Nash, Theodore Neiman, Aaron Luján, Hugo Daniel R Medicina (General) Giardia is a eukaryotic protozoal parasite with unusual characteristics, such as the absence of a morphologically evident Golgi apparatus. Although both constitutive and regulated pathways for protein secretion are evident in Giardia, little is known about the mechanisms involved in vesicular docking and fusion. In higher eukaryotes, soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) of the vesicle-associated membrane protein and syntaxin families play essential roles in these processes. In this work we identified and characterized genes for 17 SNAREs in Giardia to define the minimal set of subcellular organelles present during growth and encystation, in particular the presence or not of a Golgi apparatus. Expression and localization of all Giardia SNAREs demonstrate their presence in distinct subcellular compartments, which may represent the extent of the endomembrane system in eukaryotes. Remarkably, Giardia SNAREs, homologous to Golgi SNAREs from other organisms, do not allow the detection of a typical Golgi apparatus in either proliferating or differentiating trophozoites. However, some features of the Golgi, such as the packaging and sorting function, seem to be performed by the endoplasmic reticulum and/or the nuclear envelope. Moreover, depletion of individual genes demonstrated that several SNAREs are essential for viability, whereas others are dispensable. Thus, Giardia requires a smaller number of SNAREs compared with other eukaryotes to accomplish all of the vesicle trafficking events that are critical for the growth and differentiation of this important human pathogen. 2008-12-19 application/pdf spa http://pa.bibdigital.ucc.edu.ar/4044/1/A_Elias_Quiroga_Gottig_Nakanishi_Nash_Neiman_Lujan.pdf Elías, Eliana Vanina ORCID: https://orcid.org/0000-0003-2516-9219 <https://orcid.org/0000-0003-2516-9219>, Quiroga, Rodrigo ORCID: https://orcid.org/0000-0001-5015-0531 <https://orcid.org/0000-0001-5015-0531>, Gottig, Natalia ORCID: https://orcid.org/0000-0002-8730-8248 <https://orcid.org/0000-0002-8730-8248>, Nakanishi, Hideki ORCID: https://orcid.org/0000-0001-6968-2335 <https://orcid.org/0000-0001-6968-2335>, Nash, Theodore ORCID: https://orcid.org/0000-0003-4619-0068 <https://orcid.org/0000-0003-4619-0068>, Neiman, Aaron ORCID: https://orcid.org/0000-0002-6600-6996 <https://orcid.org/0000-0002-6600-6996> and Luján, Hugo Daniel ORCID: https://orcid.org/0000-0002-3797-8315 <https://orcid.org/0000-0002-3797-8315> (2008) Characterization of SNAREs determines the absence of a typical Golgi apparatus in the ancient eukaryote Giardia lamblia. Journal of Biological Chemistry, 283 (51). pp. 35996-36010. ISSN 1083-351X info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M806545200 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/acceptedVersion Fil: Elias, Eliana V. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina Fil: Quiroga, Rodrigo. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina Fil: Gottig, Natalia. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina Fil: Nakanishi, Hideki. Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794, United States Fil: Nash, Theodore. Laboratory of Parasitic Diseases, National Institutes of Health, NIAID, Bethesda, MD 20892, United States Fil: Neiman, Aaron. Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794, United States Fil: Luján, Hugo Daniel. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic R Medicina (General)
spellingShingle R Medicina (General)
Elías, Eliana Vanina
Quiroga, Rodrigo
Gottig, Natalia
Nakanishi, Hideki
Nash, Theodore
Neiman, Aaron
Luján, Hugo Daniel
Characterization of SNAREs determines the absence of a typical Golgi apparatus in the ancient eukaryote Giardia lamblia
topic_facet R Medicina (General)
description Giardia is a eukaryotic protozoal parasite with unusual characteristics, such as the absence of a morphologically evident Golgi apparatus. Although both constitutive and regulated pathways for protein secretion are evident in Giardia, little is known about the mechanisms involved in vesicular docking and fusion. In higher eukaryotes, soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) of the vesicle-associated membrane protein and syntaxin families play essential roles in these processes. In this work we identified and characterized genes for 17 SNAREs in Giardia to define the minimal set of subcellular organelles present during growth and encystation, in particular the presence or not of a Golgi apparatus. Expression and localization of all Giardia SNAREs demonstrate their presence in distinct subcellular compartments, which may represent the extent of the endomembrane system in eukaryotes. Remarkably, Giardia SNAREs, homologous to Golgi SNAREs from other organisms, do not allow the detection of a typical Golgi apparatus in either proliferating or differentiating trophozoites. However, some features of the Golgi, such as the packaging and sorting function, seem to be performed by the endoplasmic reticulum and/or the nuclear envelope. Moreover, depletion of individual genes demonstrated that several SNAREs are essential for viability, whereas others are dispensable. Thus, Giardia requires a smaller number of SNAREs compared with other eukaryotes to accomplish all of the vesicle trafficking events that are critical for the growth and differentiation of this important human pathogen.
format Artículo
Artículo
acceptedVersion
author Elías, Eliana Vanina
Quiroga, Rodrigo
Gottig, Natalia
Nakanishi, Hideki
Nash, Theodore
Neiman, Aaron
Luján, Hugo Daniel
author_facet Elías, Eliana Vanina
Quiroga, Rodrigo
Gottig, Natalia
Nakanishi, Hideki
Nash, Theodore
Neiman, Aaron
Luján, Hugo Daniel
author_sort Elías, Eliana Vanina
title Characterization of SNAREs determines the absence of a typical Golgi apparatus in the ancient eukaryote Giardia lamblia
title_short Characterization of SNAREs determines the absence of a typical Golgi apparatus in the ancient eukaryote Giardia lamblia
title_full Characterization of SNAREs determines the absence of a typical Golgi apparatus in the ancient eukaryote Giardia lamblia
title_fullStr Characterization of SNAREs determines the absence of a typical Golgi apparatus in the ancient eukaryote Giardia lamblia
title_full_unstemmed Characterization of SNAREs determines the absence of a typical Golgi apparatus in the ancient eukaryote Giardia lamblia
title_sort characterization of snares determines the absence of a typical golgi apparatus in the ancient eukaryote giardia lamblia
publishDate 2008
url http://pa.bibdigital.ucc.edu.ar/4044/1/A_Elias_Quiroga_Gottig_Nakanishi_Nash_Neiman_Lujan.pdf
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