Microbiological screening test validation for detection of tylosin excretion in milk of cows with low and high somatic cell counts

Antibiotic residues in milk above tolerance levels interfere with dairy product processing and pose potential health risks to consumers. Residue avoidance programmes include, among other components, the observance of withdrawal times indicated in label instructions. Persistence of antibiotics in mil...

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Autores principales: Litterio, Nicolás Javier, Calvinho, Luis Fernando, Flores, M.M., Tarabla, Héctor, Boggio, Juan Carlos
Formato: Artículo
Lenguaje:Español
Publicado: Wiley-Blackwell 2007
Materias:
SF Cultura de los animales
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/4036/1/A_Litterio_Calvinho_Flores_Tarabla_Boggio.pdf
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Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-4036
record_format dspace
spelling I38-R144-40362025-04-10T22:32:07Z http://pa.bibdigital.ucc.edu.ar/4036/ Microbiological screening test validation for detection of tylosin excretion in milk of cows with low and high somatic cell counts Litterio, Nicolás Javier Calvinho, Luis Fernando Flores, M.M. Tarabla, Héctor Boggio, Juan Carlos SF Cultura de los animales Antibiotic residues in milk above tolerance levels interfere with dairy product processing and pose potential health risks to consumers. Residue avoidance programmes include, among other components, the observance of withdrawal times indicated in label instructions. Persistence of antibiotics in milk following treatment is influenced by drug, dosage, route of administration, body weight and mammary gland health status. Compositional changes that take place during intramammary infection (IMI) can affect antibiotic excretion in milk, thus modifying milk withdrawal time. The objectives of this study were to validate sensitivity and specificity of a qualitative microbiological method (Charm® AIM-96) to detect tylosin in bovine composite milk and to determine the influence of subclinical IMI in tylosin excretion following intramuscular administration. For test validation, two groups of approximately 120 cows were used; one received a single intramuscular injection of tylosin tartrate at a dose of 20 mg/kg, while the other group remained as untreated control. Test sensitivity and specificity were 100% and 94.1% respectively. To determine the influence of subclinical IMI in tylosin excretion, two groups of seven cows, one with somatic cell counts (SCC) ≤250 000 cells/ml and the other with SCC ≥900 000, were administered a single intramuscular injection of tylosin tartrate at a dose of 20 mg/kg. Milk samples were obtained every 12 h for 10 days following treatment. Milk tylosin excretion averaged between 5 and 9 days for cows with low and high SCC respectively (P < 0.0001). Compositional changes in cows with high SCC most likely affect the pharmacokinetic characteristics of tylosin, extending the presence of the antibiotic in milk, thus influencing milk withdrawal times. Wiley-Blackwell 2007-02-28 info:eu-repo/semantics/article info:eu-repo/semantics/closedAccess application/pdf spa http://pa.bibdigital.ucc.edu.ar/4036/1/A_Litterio_Calvinho_Flores_Tarabla_Boggio.pdf Litterio, Nicolás Javier ORCID: https://orcid.org/0000-0002-8114-0579 <https://orcid.org/0000-0002-8114-0579>, Calvinho, Luis Fernando, Flores, M.M., Tarabla, Héctor ORCID: https://orcid.org/0000-0001-6442-0213 <https://orcid.org/0000-0001-6442-0213> and Boggio, Juan Carlos ORCID: https://orcid.org/0000-0001-9748-001X <https://orcid.org/0000-0001-9748-001X> (2007) Microbiological screening test validation for detection of tylosin excretion in milk of cows with low and high somatic cell counts. Journal of Veterinary Medicine Series A: physiology Pathology Clinical Medicine, 54 (1). pp. 30-35. ISSN 1439-0442 info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1439-0442.2007.00901.x
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic SF Cultura de los animales
spellingShingle SF Cultura de los animales
Litterio, Nicolás Javier
Calvinho, Luis Fernando
Flores, M.M.
Tarabla, Héctor
Boggio, Juan Carlos
Microbiological screening test validation for detection of tylosin excretion in milk of cows with low and high somatic cell counts
topic_facet SF Cultura de los animales
description Antibiotic residues in milk above tolerance levels interfere with dairy product processing and pose potential health risks to consumers. Residue avoidance programmes include, among other components, the observance of withdrawal times indicated in label instructions. Persistence of antibiotics in milk following treatment is influenced by drug, dosage, route of administration, body weight and mammary gland health status. Compositional changes that take place during intramammary infection (IMI) can affect antibiotic excretion in milk, thus modifying milk withdrawal time. The objectives of this study were to validate sensitivity and specificity of a qualitative microbiological method (Charm® AIM-96) to detect tylosin in bovine composite milk and to determine the influence of subclinical IMI in tylosin excretion following intramuscular administration. For test validation, two groups of approximately 120 cows were used; one received a single intramuscular injection of tylosin tartrate at a dose of 20 mg/kg, while the other group remained as untreated control. Test sensitivity and specificity were 100% and 94.1% respectively. To determine the influence of subclinical IMI in tylosin excretion, two groups of seven cows, one with somatic cell counts (SCC) ≤250 000 cells/ml and the other with SCC ≥900 000, were administered a single intramuscular injection of tylosin tartrate at a dose of 20 mg/kg. Milk samples were obtained every 12 h for 10 days following treatment. Milk tylosin excretion averaged between 5 and 9 days for cows with low and high SCC respectively (P < 0.0001). Compositional changes in cows with high SCC most likely affect the pharmacokinetic characteristics of tylosin, extending the presence of the antibiotic in milk, thus influencing milk withdrawal times.
format Artículo
author Litterio, Nicolás Javier
Calvinho, Luis Fernando
Flores, M.M.
Tarabla, Héctor
Boggio, Juan Carlos
author_facet Litterio, Nicolás Javier
Calvinho, Luis Fernando
Flores, M.M.
Tarabla, Héctor
Boggio, Juan Carlos
author_sort Litterio, Nicolás Javier
title Microbiological screening test validation for detection of tylosin excretion in milk of cows with low and high somatic cell counts
title_short Microbiological screening test validation for detection of tylosin excretion in milk of cows with low and high somatic cell counts
title_full Microbiological screening test validation for detection of tylosin excretion in milk of cows with low and high somatic cell counts
title_fullStr Microbiological screening test validation for detection of tylosin excretion in milk of cows with low and high somatic cell counts
title_full_unstemmed Microbiological screening test validation for detection of tylosin excretion in milk of cows with low and high somatic cell counts
title_sort microbiological screening test validation for detection of tylosin excretion in milk of cows with low and high somatic cell counts
publisher Wiley-Blackwell
publishDate 2007
url http://pa.bibdigital.ucc.edu.ar/4036/1/A_Litterio_Calvinho_Flores_Tarabla_Boggio.pdf
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