ELISA method for assess liver - Kidney microsomes antibodies [Método ELISA para anticuerpos antimicrosomales hígado-riñón]
Almost twenty years ago a new type of autoimmune chronic hepatitis, denominated Type 2 Autoimmune Hepatitis was described. It is more frequent in girls and young women, characterized by the presence of serum autoantibodies liver/kidney anti microsomes type 1 (LKM-1). A microsomic enzyme of 50 kDa, t...
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| Formato: | Artículo |
| Lenguaje: | Español |
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Federación Bioquímica de la Provincia de Buenos Aires
2004
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| Acceso en línea: | http://pa.bibdigital.ucc.edu.ar/3982/1/A_Grumelli_Nicolas.pdf |
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I38-R144-39822025-04-11T18:57:14Z http://pa.bibdigital.ucc.edu.ar/3982/ ELISA method for assess liver - Kidney microsomes antibodies [Método ELISA para anticuerpos antimicrosomales hígado-riñón] Nicolas, Juan Carlos Grumelli, Yanina Alejandra QD Química Almost twenty years ago a new type of autoimmune chronic hepatitis, denominated Type 2 Autoimmune Hepatitis was described. It is more frequent in girls and young women, characterized by the presence of serum autoantibodies liver/kidney anti microsomes type 1 (LKM-1). A microsomic enzyme of 50 kDa, the Cytochrome P450 Monooxygenase (P450IID6) metabolizes drugs and is the target antigen of the LKM-1. This antigen can also be expressed in the cellular membrane of the hepatocytes modulated by interleukines and tumoral necrosis factor (TNF). The most frequent method to determine antibodies anti LKM-1 is the indirect immunofluorescence, using liver and kidney rat slides. The main inconvenience is the similar pattern that they present with other antibodies denominated Antimitochondrial (AMA). In this work, a test for LKM is developed using an Enzime-linked Immunosorbent Assay (ELISA), starting from extracted liver rat microsomes. The Interassay Variation Coefficients for two significance levels, both negative and positive-LKM samples were respectively 12.5% and 8.7%. Sensibility and specificity were 100% and 97.5% respectively for both samples. Federación Bioquímica de la Provincia de Buenos Aires 2004-03-31 info:eu-repo/semantics/article info:eu-repo/semantics/closedAccess application/pdf spa http://pa.bibdigital.ucc.edu.ar/3982/1/A_Grumelli_Nicolas.pdf Nicolas, Juan Carlos ORCID: https://orcid.org/0000-0001-9163-2668 <https://orcid.org/0000-0001-9163-2668> and Grumelli, Yanina Alejandra ORCID: https://orcid.org/0000-0001-6234-865X <https://orcid.org/0000-0001-6234-865X> (2004) ELISA method for assess liver - Kidney microsomes antibodies [Método ELISA para anticuerpos antimicrosomales hígado-riñón]. Acta Bioquímica Clínica Latinoamericana, 38 (1). pp. 23-27. ISSN 0325-2957 |
| institution |
Universidad Católica de Córdoba |
| institution_str |
I-38 |
| repository_str |
R-144 |
| collection |
Producción Académica Universidad Católica de Córdoba (UCCor) |
| language |
Español |
| orig_language_str_mv |
spa |
| topic |
QD Química |
| spellingShingle |
QD Química Nicolas, Juan Carlos Grumelli, Yanina Alejandra ELISA method for assess liver - Kidney microsomes antibodies [Método ELISA para anticuerpos antimicrosomales hígado-riñón] |
| topic_facet |
QD Química |
| description |
Almost twenty years ago a new type of autoimmune chronic hepatitis, denominated Type 2 Autoimmune Hepatitis was described. It is more frequent in girls and young women, characterized by the presence of serum autoantibodies liver/kidney anti microsomes type 1 (LKM-1). A microsomic enzyme of 50 kDa, the Cytochrome P450 Monooxygenase (P450IID6) metabolizes drugs and is the target antigen of the LKM-1. This antigen can also be expressed in the cellular membrane of the hepatocytes modulated by interleukines and tumoral necrosis factor (TNF). The most frequent method to determine antibodies anti LKM-1 is the indirect immunofluorescence, using liver and kidney rat slides. The main inconvenience is the similar pattern that they present with other antibodies denominated Antimitochondrial (AMA). In this work, a test for LKM is developed using an Enzime-linked Immunosorbent Assay (ELISA), starting from extracted liver rat microsomes. The Interassay Variation Coefficients for two significance levels, both negative and positive-LKM samples were respectively 12.5% and 8.7%. Sensibility and specificity were 100% and 97.5% respectively for both samples. |
| format |
Artículo |
| author |
Nicolas, Juan Carlos Grumelli, Yanina Alejandra |
| author_facet |
Nicolas, Juan Carlos Grumelli, Yanina Alejandra |
| author_sort |
Nicolas, Juan Carlos |
| title |
ELISA method for assess liver - Kidney microsomes antibodies
[Método ELISA para anticuerpos antimicrosomales hígado-riñón] |
| title_short |
ELISA method for assess liver - Kidney microsomes antibodies
[Método ELISA para anticuerpos antimicrosomales hígado-riñón] |
| title_full |
ELISA method for assess liver - Kidney microsomes antibodies
[Método ELISA para anticuerpos antimicrosomales hígado-riñón] |
| title_fullStr |
ELISA method for assess liver - Kidney microsomes antibodies
[Método ELISA para anticuerpos antimicrosomales hígado-riñón] |
| title_full_unstemmed |
ELISA method for assess liver - Kidney microsomes antibodies
[Método ELISA para anticuerpos antimicrosomales hígado-riñón] |
| title_sort |
elisa method for assess liver - kidney microsomes antibodies
[método elisa para anticuerpos antimicrosomales hígado-riñón] |
| publisher |
Federación Bioquímica de la Provincia de Buenos Aires |
| publishDate |
2004 |
| url |
http://pa.bibdigital.ucc.edu.ar/3982/1/A_Grumelli_Nicolas.pdf |
| work_keys_str_mv |
AT nicolasjuancarlos elisamethodforassessliverkidneymicrosomesantibodiesmetodoelisaparaanticuerposantimicrosomaleshigadorinon AT grumelliyaninaalejandra elisamethodforassessliverkidneymicrosomesantibodiesmetodoelisaparaanticuerposantimicrosomaleshigadorinon |
| _version_ |
1832592419054419968 |