Experimental retinopathy of prematurity: angiostatic inhibition by nimodipine, ginkgo-biloba, and dipyridamole, and response to different growth factors

PURPOSE. To investigate whether commonly used vasodilating drugs ameliorate angiogenesis in experimental retinopathy of prematurity (ROP), and to study the response of these drugs to different growth factors. METHODS. We used a rat and mouse model of oxygen-induced ischemic retinopathy. Animals were...

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Autores principales: Juárez Beltrán, Claudio Patricio, Muiño, Juan Carlos, Guglielmone, H., Sambuelli, Rubén Horacio, Echenique, J.R., Hernández, M., Luna, J.D.
Formato: Artículo
Lenguaje:Español
Publicado: 2000
Materias:
RE Oftalmología
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/3920/1/A_Ju%C3%A1rezBeltr%C3%A1n_Mui%C3%B1o_Guglielmone_Sambuelli_Echenique_%20Hern%C3%A1ndez_Luna.pdf
Aporte de:
Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-3920
record_format dspace
spelling I38-R144-39202025-04-10T20:56:44Z http://pa.bibdigital.ucc.edu.ar/3920/ Experimental retinopathy of prematurity: angiostatic inhibition by nimodipine, ginkgo-biloba, and dipyridamole, and response to different growth factors Juárez Beltrán, Claudio Patricio Muiño, Juan Carlos Guglielmone, H. Sambuelli, Rubén Horacio Echenique, J.R. Hernández, M. Luna, J.D. RE Oftalmología PURPOSE. To investigate whether commonly used vasodilating drugs ameliorate angiogenesis in experimental retinopathy of prematurity (ROP), and to study the response of these drugs to different growth factors. METHODS. We used a rat and mouse model of oxygen-induced ischemic retinopathy. Animals were treated with nimodipine, ginkgo-biloba and dipyridamole intraperitoneally starting the day before exposure to room air (day 1). Controls were injected with vehicle solution only. Eyes were processed histopathologically with serial sections and neovascularization was measured by counting the nuclei within the retinal internal limiting membrane, by a masked observer. Retinal and vitreous tissues were assayed by ELISA for VEGF, PDGF and TGFβ2. RESULTS. Nimodipine significantly inhibited the growth of new vessels in rats. The number of nuclei was 310 ± 69 in the control group (n:14) and 121 ± 53 in the treated ones (n:14), (p<0.0005). Similar results were found with ginkgo-biloba extracts: 344 ± 53 (n:15) in controls, and 136 ± 29 (n:11) in treated ones (p<0.0005), and with dipyridamole: 303 ± 69 (n:13) in controls, and 131 ± 48.5 in treated rats (p<0.0005). Results were similar in mice. 186 ± 45 (n:7) nuclei counted in controls against 90 ± 25 (n:6) for dipyridamole treated (p<0.0005); and 81 ± 21 for ginkgo-biloba treated animals (p <0.0005). A gradual, very significant increase in VEGF values in response to relative hypoxia (room air) contrasted with the significant-inhibition noted both with ginkgo-biloba extracts and dipyridamole. TGFβ2 and PDGF both showed a gradual increase in relative hypoxia at days 2 and 4 of room air (12[removed]. 2000-12-31 info:eu-repo/semantics/article info:eu-repo/semantics/closedAccess application/pdf spa http://pa.bibdigital.ucc.edu.ar/3920/1/A_Ju%C3%A1rezBeltr%C3%A1n_Mui%C3%B1o_Guglielmone_Sambuelli_Echenique_%20Hern%C3%A1ndez_Luna.pdf Juárez Beltrán, Claudio Patricio, Muiño, Juan Carlos, Guglielmone, H., Sambuelli, Rubén Horacio, Echenique, J.R., Hernández, M. and Luna, J.D. (2000) Experimental retinopathy of prematurity: angiostatic inhibition by nimodipine, ginkgo-biloba, and dipyridamole, and response to different growth factors. European Journal of Ophthalmology, 10 (1). pp. 51-59. ISSN 1120-6721 info:eu-repo/semantics/altIdentifier/doi/10.1177/112067210001000109
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic RE Oftalmología
spellingShingle RE Oftalmología
Juárez Beltrán, Claudio Patricio
Muiño, Juan Carlos
Guglielmone, H.
Sambuelli, Rubén Horacio
Echenique, J.R.
Hernández, M.
Luna, J.D.
Experimental retinopathy of prematurity: angiostatic inhibition by nimodipine, ginkgo-biloba, and dipyridamole, and response to different growth factors
topic_facet RE Oftalmología
description PURPOSE. To investigate whether commonly used vasodilating drugs ameliorate angiogenesis in experimental retinopathy of prematurity (ROP), and to study the response of these drugs to different growth factors. METHODS. We used a rat and mouse model of oxygen-induced ischemic retinopathy. Animals were treated with nimodipine, ginkgo-biloba and dipyridamole intraperitoneally starting the day before exposure to room air (day 1). Controls were injected with vehicle solution only. Eyes were processed histopathologically with serial sections and neovascularization was measured by counting the nuclei within the retinal internal limiting membrane, by a masked observer. Retinal and vitreous tissues were assayed by ELISA for VEGF, PDGF and TGFβ2. RESULTS. Nimodipine significantly inhibited the growth of new vessels in rats. The number of nuclei was 310 ± 69 in the control group (n:14) and 121 ± 53 in the treated ones (n:14), (p<0.0005). Similar results were found with ginkgo-biloba extracts: 344 ± 53 (n:15) in controls, and 136 ± 29 (n:11) in treated ones (p<0.0005), and with dipyridamole: 303 ± 69 (n:13) in controls, and 131 ± 48.5 in treated rats (p<0.0005). Results were similar in mice. 186 ± 45 (n:7) nuclei counted in controls against 90 ± 25 (n:6) for dipyridamole treated (p<0.0005); and 81 ± 21 for ginkgo-biloba treated animals (p <0.0005). A gradual, very significant increase in VEGF values in response to relative hypoxia (room air) contrasted with the significant-inhibition noted both with ginkgo-biloba extracts and dipyridamole. TGFβ2 and PDGF both showed a gradual increase in relative hypoxia at days 2 and 4 of room air (12[removed].
format Artículo
author Juárez Beltrán, Claudio Patricio
Muiño, Juan Carlos
Guglielmone, H.
Sambuelli, Rubén Horacio
Echenique, J.R.
Hernández, M.
Luna, J.D.
author_facet Juárez Beltrán, Claudio Patricio
Muiño, Juan Carlos
Guglielmone, H.
Sambuelli, Rubén Horacio
Echenique, J.R.
Hernández, M.
Luna, J.D.
author_sort Juárez Beltrán, Claudio Patricio
title Experimental retinopathy of prematurity: angiostatic inhibition by nimodipine, ginkgo-biloba, and dipyridamole, and response to different growth factors
title_short Experimental retinopathy of prematurity: angiostatic inhibition by nimodipine, ginkgo-biloba, and dipyridamole, and response to different growth factors
title_full Experimental retinopathy of prematurity: angiostatic inhibition by nimodipine, ginkgo-biloba, and dipyridamole, and response to different growth factors
title_fullStr Experimental retinopathy of prematurity: angiostatic inhibition by nimodipine, ginkgo-biloba, and dipyridamole, and response to different growth factors
title_full_unstemmed Experimental retinopathy of prematurity: angiostatic inhibition by nimodipine, ginkgo-biloba, and dipyridamole, and response to different growth factors
title_sort experimental retinopathy of prematurity: angiostatic inhibition by nimodipine, ginkgo-biloba, and dipyridamole, and response to different growth factors
publishDate 2000
url http://pa.bibdigital.ucc.edu.ar/3920/1/A_Ju%C3%A1rezBeltr%C3%A1n_Mui%C3%B1o_Guglielmone_Sambuelli_Echenique_%20Hern%C3%A1ndez_Luna.pdf
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