Callus induction in culture of Oenothera hookeri and Oenothera picensis anthers

In the present work we try to determine optimum conditions for callus induction in anther culture of Oenothera hookeri and O. picensis. The anther callus yield was increased when the anthers were cultured on modified MS medium supplied with 2 mg dm-3 2,4-D and 2 mg dm-3 NAA, in both species. In O. h...

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Autores principales: Martínez, L.D., Noher de Halac, Inés
Formato: Artículo acceptedVersion
Lenguaje:Español
Publicado: 1997
Materias:
SB Cultura de la planta
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/3724/1/A_Mart%C3%ADnez_NoherdeHalac.pdf
Aporte de:
Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-3724
record_format dspace
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic SB Cultura de la planta
spellingShingle SB Cultura de la planta
Martínez, L.D.
Noher de Halac, Inés
Callus induction in culture of Oenothera hookeri and Oenothera picensis anthers
topic_facet SB Cultura de la planta
description In the present work we try to determine optimum conditions for callus induction in anther culture of Oenothera hookeri and O. picensis. The anther callus yield was increased when the anthers were cultured on modified MS medium supplied with 2 mg dm-3 2,4-D and 2 mg dm-3 NAA, in both species. In O. hookeri, best results were obtained when anthers were excised from 7.2 - 9 mm buds at the stage of vacuolated microspores, then pretreated at 4°C for 2 d and grown under 16-h photoperiod. The response to anther culture of O. picensis was generally very poor compared with that of O. hookeri. The higher yield of calli was obtained when anthers were excised from 6.2-8 mm buds at the stage of vacuolated microspores and grown under continuous light. The cold pretreatment of buds decreased anther response in this species.
format Artículo
Artículo
acceptedVersion
author Martínez, L.D.
Noher de Halac, Inés
author_facet Martínez, L.D.
Noher de Halac, Inés
author_sort Martínez, L.D.
title Callus induction in culture of Oenothera hookeri and Oenothera picensis anthers
title_short Callus induction in culture of Oenothera hookeri and Oenothera picensis anthers
title_full Callus induction in culture of Oenothera hookeri and Oenothera picensis anthers
title_fullStr Callus induction in culture of Oenothera hookeri and Oenothera picensis anthers
title_full_unstemmed Callus induction in culture of Oenothera hookeri and Oenothera picensis anthers
title_sort callus induction in culture of oenothera hookeri and oenothera picensis anthers
publishDate 1997
url http://pa.bibdigital.ucc.edu.ar/3724/1/A_Mart%C3%ADnez_NoherdeHalac.pdf
work_keys_str_mv AT martinezld callusinductionincultureofoenotherahookeriandoenotherapicensisanthers
AT noherdehalacines callusinductionincultureofoenotherahookeriandoenotherapicensisanthers
bdutipo_str Repositorios
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