Organogenesis of anther-derived calluses in long-term cultures of Oenothera hookeri de Vries
Anthers of O. hookeri containing uninucleate microspores were cultured, in vitro, at 25°C (16 hours photoperiod) on solid MS medium. After 10-15 days, on media with 2,4-dichlorophenoxyacetic acid, 1-naphthaleneacetic acid and 6-benzylaminopurine, anthers developed friable calluses. After unsuccessfu...
Guardado en:
| Autores principales: | , |
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| Formato: | Artículo |
| Lenguaje: | Español |
| Publicado: |
1995
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| Materias: | |
| Acceso en línea: | http://pa.bibdigital.ucc.edu.ar/3716/1/A_Mart%C3%ADnez_NoherdeHalac.pdf |
| Aporte de: |
| Sumario: | Anthers of O. hookeri containing uninucleate microspores were cultured, in vitro, at 25°C (16 hours photoperiod) on solid MS medium. After 10-15 days, on media with 2,4-dichlorophenoxyacetic acid, 1-naphthaleneacetic acid and 6-benzylaminopurine, anthers developed friable calluses. After unsuccessful treatments on embryogenic-and/or organogenic-induction media, calluses were placed on a hormone-free MS medium for 24 months with routine transfers every 3 weeks. After this period, the calluses developed buds and subsequently plants. Ro generation plants, were morphologically distinct. |
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