Organogenesis of anther-derived calluses in long-term cultures of Oenothera hookeri de Vries

Anthers of O. hookeri containing uninucleate microspores were cultured, in vitro, at 25°C (16 hours photoperiod) on solid MS medium. After 10-15 days, on media with 2,4-dichlorophenoxyacetic acid, 1-naphthaleneacetic acid and 6-benzylaminopurine, anthers developed friable calluses. After unsuccessfu...

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Autores principales: Martínez, Lidia Dora, Noher de Halac, Inés
Formato: Artículo
Lenguaje:Español
Publicado: 1995
Materias:
SB Cultura de la planta
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/3716/1/A_Mart%C3%ADnez_NoherdeHalac.pdf
Aporte de:
Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-3716
record_format dspace
spelling I38-R144-37162025-04-11T18:48:10Z http://pa.bibdigital.ucc.edu.ar/3716/ Organogenesis of anther-derived calluses in long-term cultures of Oenothera hookeri de Vries Martínez, Lidia Dora Noher de Halac, Inés SB Cultura de la planta Anthers of O. hookeri containing uninucleate microspores were cultured, in vitro, at 25°C (16 hours photoperiod) on solid MS medium. After 10-15 days, on media with 2,4-dichlorophenoxyacetic acid, 1-naphthaleneacetic acid and 6-benzylaminopurine, anthers developed friable calluses. After unsuccessful treatments on embryogenic-and/or organogenic-induction media, calluses were placed on a hormone-free MS medium for 24 months with routine transfers every 3 weeks. After this period, the calluses developed buds and subsequently plants. Ro generation plants, were morphologically distinct. 1995-07-31 info:eu-repo/semantics/article info:eu-repo/semantics/closedAccess application/pdf spa http://pa.bibdigital.ucc.edu.ar/3716/1/A_Mart%C3%ADnez_NoherdeHalac.pdf Martínez, Lidia Dora and Noher de Halac, Inés ORCID: https://orcid.org/0000-0003-2930-9282 <https://orcid.org/0000-0003-2930-9282> (1995) Organogenesis of anther-derived calluses in long-term cultures of Oenothera hookeri de Vries. Plant Cell, Tissue and Organ Culture, 42 (1). pp. 91-96. ISSN 1573-5044 info:eu-repo/semantics/altIdentifier/doi/10.1007/BF00037686
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic SB Cultura de la planta
spellingShingle SB Cultura de la planta
Martínez, Lidia Dora
Noher de Halac, Inés
Organogenesis of anther-derived calluses in long-term cultures of Oenothera hookeri de Vries
topic_facet SB Cultura de la planta
description Anthers of O. hookeri containing uninucleate microspores were cultured, in vitro, at 25°C (16 hours photoperiod) on solid MS medium. After 10-15 days, on media with 2,4-dichlorophenoxyacetic acid, 1-naphthaleneacetic acid and 6-benzylaminopurine, anthers developed friable calluses. After unsuccessful treatments on embryogenic-and/or organogenic-induction media, calluses were placed on a hormone-free MS medium for 24 months with routine transfers every 3 weeks. After this period, the calluses developed buds and subsequently plants. Ro generation plants, were morphologically distinct.
format Artículo
author Martínez, Lidia Dora
Noher de Halac, Inés
author_facet Martínez, Lidia Dora
Noher de Halac, Inés
author_sort Martínez, Lidia Dora
title Organogenesis of anther-derived calluses in long-term cultures of Oenothera hookeri de Vries
title_short Organogenesis of anther-derived calluses in long-term cultures of Oenothera hookeri de Vries
title_full Organogenesis of anther-derived calluses in long-term cultures of Oenothera hookeri de Vries
title_fullStr Organogenesis of anther-derived calluses in long-term cultures of Oenothera hookeri de Vries
title_full_unstemmed Organogenesis of anther-derived calluses in long-term cultures of Oenothera hookeri de Vries
title_sort organogenesis of anther-derived calluses in long-term cultures of oenothera hookeri de vries
publishDate 1995
url http://pa.bibdigital.ucc.edu.ar/3716/1/A_Mart%C3%ADnez_NoherdeHalac.pdf
work_keys_str_mv AT martinezlidiadora organogenesisofantherderivedcallusesinlongtermculturesofoenotherahookeridevries
AT noherdehalacines organogenesisofantherderivedcallusesinlongtermculturesofoenotherahookeridevries
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