Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf

Splenic autoimplantation appears to be the only alternative to preserve splenic tissue after splenectomy; however, its relevance is still controversial. We intended to study splenic autoimplantation in the greater omentum and stomach wall of rabbits and analyze its hematoimmunological performance an...

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Autores principales: Álvarez, Fernando Andrés, Colombres, Gustavo Adolfo, Viqueira, Alejandro, Maldonado, Jorge Eduardo, Alvarellos, Emilio, Sambuelli, Rubén Horacio, Alvarellos, Teresita
Formato: Artículo acceptedVersion
Lenguaje:Español
Publicado: Sociedad Argentina de Gastroenterología 2010
Materias:
R Medicina (General)
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/3443/1/A_%C3%81lvarez_Colombres_Viqueira_Maldonado_Alvarellos_Sambuelli_Alvarellos.pdf
Aporte de:
Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-3443
record_format dspace
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic R Medicina (General)
spellingShingle R Medicina (General)
Álvarez, Fernando Andrés
Colombres, Gustavo Adolfo
Viqueira, Alejandro
Maldonado, Jorge Eduardo
Alvarellos, Emilio
Sambuelli, Rubén Horacio
Alvarellos, Teresita
Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf
topic_facet R Medicina (General)
description Splenic autoimplantation appears to be the only alternative to preserve splenic tissue after splenectomy; however, its relevance is still controversial. We intended to study splenic autoimplantation in the greater omentum and stomach wall of rabbits and analyze its hematoimmunological performance and the preservation of original structures. Methods. New Zealand rabbits were divided in two groups: autoimplanted (A) (n=13) and splenectomized (S) (n=4). The animals of group A underwent autoimplantation of splenic fragments in the greater omentum and gastric wall. Both groups were evaluated by hemocytological tests, scintigraphy, immunoglobulin and C3 dosages, before the surgery and 2 and 4 months afterwards. After 4 months, the grafts were removed and histological examination and gen rearrangement of B-lymphocytes receptors by polymerase chain reaction (PCR) were performed to assess the cellular diversity of clones. Results. The histological analysis demonstrated the presence of splenic tissue in 10 of the 13 cases (77%) with evident size reduction. The gastric location did not develop complications and demonstrated higher morphological correspondence to the autoimplanted tissue. Both groups showed significant decrease of IgM and increase of C3, without considerable differences between both of them during follow up. From the 8 grafts studied with PCR, 3 cases presented polyclonality and 5 oligoclonality. Conclusions. The revascularized grafts evidenced splenic regenerating tissue, probably associated to the oligoclonality detected by PCR. Consequently, we consider that autoimplantation is a reasonable alternative for splenectomized patients, even though the stomach placement and the high frequency of oligoclonality justify further investigation.
format Artículo
Artículo
acceptedVersion
author Álvarez, Fernando Andrés
Colombres, Gustavo Adolfo
Viqueira, Alejandro
Maldonado, Jorge Eduardo
Alvarellos, Emilio
Sambuelli, Rubén Horacio
Alvarellos, Teresita
author_facet Álvarez, Fernando Andrés
Colombres, Gustavo Adolfo
Viqueira, Alejandro
Maldonado, Jorge Eduardo
Alvarellos, Emilio
Sambuelli, Rubén Horacio
Alvarellos, Teresita
author_sort Álvarez, Fernando Andrés
title Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf
title_short Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf
title_full Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf
title_fullStr Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf
title_full_unstemmed Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf
title_sort splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and b-cell repertoire in the graf
publisher Sociedad Argentina de Gastroenterología
publishDate 2010
url http://pa.bibdigital.ucc.edu.ar/3443/1/A_%C3%81lvarez_Colombres_Viqueira_Maldonado_Alvarellos_Sambuelli_Alvarellos.pdf
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