Elucidating potential effectors, pathogenicity and virulence factors expressed by the phytopathogenic fungus Thecaphora frezii through analysis of its transcriptome

Thecaphora frezii is a phytopathogenic fungus belonging to the Ustilaginomycetes class, which causes peanut smut disease. In its biological cycle it has three structures, teliospores (is the resistance structure), basidiospores and hyphae. The mycelium is the structure that penetrates the plant’s gy...

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Autores principales: Soria, Néstor Walter, Badariotti, Esteban Hernán, Alasino, Valeria R., Figueroa, Ana Cristina, Díaz, María S., Yang, Pablo, Beltramo, Dante Miguel
Formato: Artículo
Lenguaje:Español
Publicado: Springer Science and Business Media B.V. 2022
Materias:
Q Ciencia (General)
QR355 Virología
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/3317/1/A_Soria_Badariotti_Alasino.pdf
Aporte de:
Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-3317
record_format dspace
spelling I38-R144-33172025-10-23T13:28:47Z http://pa.bibdigital.ucc.edu.ar/3317/ Elucidating potential effectors, pathogenicity and virulence factors expressed by the phytopathogenic fungus Thecaphora frezii through analysis of its transcriptome Soria, Néstor Walter Badariotti, Esteban Hernán Alasino, Valeria R. Figueroa, Ana Cristina Díaz, María S. Yang, Pablo Beltramo, Dante Miguel Q Ciencia (General) QR355 Virología Thecaphora frezii is a phytopathogenic fungus belonging to the Ustilaginomycetes class, which causes peanut smut disease. In its biological cycle it has three structures, teliospores (is the resistance structure), basidiospores and hyphae. The mycelium is the structure that penetrates the plant’s gynophore and initiates the infection. For this action, the expression of effectors, pathogenicity and virulence factor proteins is necessary. The aim of this study was to identify potential Thecaphora frezii’s proteins that could exert pathogenicity, virulence and/or effector functions. Based on the transcriptome of two ontogenetic stages (teliospore and hyphae) of Thecaphora frezii, and a series of bioinformatic analyses, 18 potential effectors and 91 factors possibly involved in the level of pathogenicity and virulence were identified. Higher expression levels of candidate effectors were found in the infective stage of the fungus. In the other hand, the major variability observed in pathogenicity and virulence factors expressed between Ustilago maydis and Thecaphora frezii were the number of virulence factors secreted (higher in U. maydis). In the future, when the genome of the fungus will be known, functional studies of these proteins could be carried out to validate their function. Springer Science and Business Media B.V. 2022-08-17 info:eu-repo/semantics/article info:eu-repo/semantics/closedAccess application/pdf spa http://pa.bibdigital.ucc.edu.ar/3317/1/A_Soria_Badariotti_Alasino.pdf Soria, Néstor Walter ORCID: https://orcid.org/0000-0002-8232-1822 <https://orcid.org/0000-0002-8232-1822>, Badariotti, Esteban Hernán ORCID: https://orcid.org/0000-0003-2512-6622 <https://orcid.org/0000-0003-2512-6622>, Alasino, Valeria R., Figueroa, Ana Cristina ORCID: https://orcid.org/0000-0002-9567-4192 <https://orcid.org/0000-0002-9567-4192>, Díaz, María S., Yang, Pablo ORCID: https://orcid.org/0000-0001-5206-8873 <https://orcid.org/0000-0001-5206-8873> and Beltramo, Dante Miguel ORCID: https://orcid.org/0000-0002-8198-3525 <https://orcid.org/0000-0002-8198-3525> (2022) Elucidating potential effectors, pathogenicity and virulence factors expressed by the phytopathogenic fungus Thecaphora frezii through analysis of its transcriptome. European Journal of Plant Pathology. ISSN 0929-1873 https://link.springer.com/article/10.1007/s10658-022-02562-2 info:eu-repo/semantics/altIdentifier/doi/10.1007/s10658-022-02562-2
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic Q Ciencia (General)
QR355 Virología
spellingShingle Q Ciencia (General)
QR355 Virología
Soria, Néstor Walter
Badariotti, Esteban Hernán
Alasino, Valeria R.
Figueroa, Ana Cristina
Díaz, María S.
Yang, Pablo
Beltramo, Dante Miguel
Elucidating potential effectors, pathogenicity and virulence factors expressed by the phytopathogenic fungus Thecaphora frezii through analysis of its transcriptome
topic_facet Q Ciencia (General)
QR355 Virología
description Thecaphora frezii is a phytopathogenic fungus belonging to the Ustilaginomycetes class, which causes peanut smut disease. In its biological cycle it has three structures, teliospores (is the resistance structure), basidiospores and hyphae. The mycelium is the structure that penetrates the plant’s gynophore and initiates the infection. For this action, the expression of effectors, pathogenicity and virulence factor proteins is necessary. The aim of this study was to identify potential Thecaphora frezii’s proteins that could exert pathogenicity, virulence and/or effector functions. Based on the transcriptome of two ontogenetic stages (teliospore and hyphae) of Thecaphora frezii, and a series of bioinformatic analyses, 18 potential effectors and 91 factors possibly involved in the level of pathogenicity and virulence were identified. Higher expression levels of candidate effectors were found in the infective stage of the fungus. In the other hand, the major variability observed in pathogenicity and virulence factors expressed between Ustilago maydis and Thecaphora frezii were the number of virulence factors secreted (higher in U. maydis). In the future, when the genome of the fungus will be known, functional studies of these proteins could be carried out to validate their function.
format Artículo
author Soria, Néstor Walter
Badariotti, Esteban Hernán
Alasino, Valeria R.
Figueroa, Ana Cristina
Díaz, María S.
Yang, Pablo
Beltramo, Dante Miguel
author_facet Soria, Néstor Walter
Badariotti, Esteban Hernán
Alasino, Valeria R.
Figueroa, Ana Cristina
Díaz, María S.
Yang, Pablo
Beltramo, Dante Miguel
author_sort Soria, Néstor Walter
title Elucidating potential effectors, pathogenicity and virulence factors expressed by the phytopathogenic fungus Thecaphora frezii through analysis of its transcriptome
title_short Elucidating potential effectors, pathogenicity and virulence factors expressed by the phytopathogenic fungus Thecaphora frezii through analysis of its transcriptome
title_full Elucidating potential effectors, pathogenicity and virulence factors expressed by the phytopathogenic fungus Thecaphora frezii through analysis of its transcriptome
title_fullStr Elucidating potential effectors, pathogenicity and virulence factors expressed by the phytopathogenic fungus Thecaphora frezii through analysis of its transcriptome
title_full_unstemmed Elucidating potential effectors, pathogenicity and virulence factors expressed by the phytopathogenic fungus Thecaphora frezii through analysis of its transcriptome
title_sort elucidating potential effectors, pathogenicity and virulence factors expressed by the phytopathogenic fungus thecaphora frezii through analysis of its transcriptome
publisher Springer Science and Business Media B.V.
publishDate 2022
url http://pa.bibdigital.ucc.edu.ar/3317/1/A_Soria_Badariotti_Alasino.pdf
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