Evaluation of MYC Gene Amplification in Prostate Cancer Using a Dual Color Chromogenic In Situ Hybridization (Dual CISH) Assay

Objective: The overall purpose of the study was to demonstrate applicability of the DAKO dual-color chromogenic in situ hybridization (CISH) assay (DAKO Denmark, Glostrup) with respect to fluorescence in situ hybridization (FISH) probes MYC-C. Methods: MYC gene amplification by FISH and DAKO dual...

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Detalles Bibliográficos
Autores principales: Lerda, Daniel Enrique, Cabrera, Marta, Flores, Jorge, Gutierrez, Luis, Chierichetti, Armando, Paladini, Mario, Revol, Martín, García Öntö, Hernán
Formato: Artículo acceptedVersion
Lenguaje:Inglés
Publicado: Publishing Company in Wilmington 2013
Materias:
R Medicina (General)
RC0254 Neoplasias. Los tumores. Oncología (incluyendo cáncer)
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/1651/1/A_Lerda_Cabrera_Flores.pdf
Aporte de:
Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-1651
record_format dspace
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Inglés
orig_language_str_mv eng
topic R Medicina (General)
RC0254 Neoplasias. Los tumores. Oncología (incluyendo cáncer)
spellingShingle R Medicina (General)
RC0254 Neoplasias. Los tumores. Oncología (incluyendo cáncer)
Lerda, Daniel Enrique
Cabrera, Marta
Flores, Jorge
Gutierrez, Luis
Chierichetti, Armando
Paladini, Mario
Revol, Martín
García Öntö, Hernán
Evaluation of MYC Gene Amplification in Prostate Cancer Using a Dual Color Chromogenic In Situ Hybridization (Dual CISH) Assay
topic_facet R Medicina (General)
RC0254 Neoplasias. Los tumores. Oncología (incluyendo cáncer)
description Objective: The overall purpose of the study was to demonstrate applicability of the DAKO dual-color chromogenic in situ hybridization (CISH) assay (DAKO Denmark, Glostrup) with respect to fluorescence in situ hybridization (FISH) probes MYC-C. Methods: MYC gene amplification by FISH and DAKO dual-color CISH Results: The study showed that the dual-color CISH assay can convert Texas red and fluorescein isothiocyanate (FITC) signals into chromogenic signals. The dual –color CISH assay was performed on 40 cases of prostate cancer. Amplification was identified in 12 of 40 (30%) tumors. No amplification was seen in 28 of 40 (70%) tumors. FISH data were available in total of 40 tumors. All tumors showed concordant results between dual-color CISH and FISH for classifying a tumor as MYC amplified or not amplified. Conclusions: We conclude that dual-color DAKO CISH assay is an accurate method for determining MYC gene amplification with added advantages that make it a more practically useful method.
format Artículo
Artículo
acceptedVersion
author Lerda, Daniel Enrique
Cabrera, Marta
Flores, Jorge
Gutierrez, Luis
Chierichetti, Armando
Paladini, Mario
Revol, Martín
García Öntö, Hernán
author_facet Lerda, Daniel Enrique
Cabrera, Marta
Flores, Jorge
Gutierrez, Luis
Chierichetti, Armando
Paladini, Mario
Revol, Martín
García Öntö, Hernán
author_sort Lerda, Daniel Enrique
title Evaluation of MYC Gene Amplification in Prostate Cancer Using a Dual Color Chromogenic In Situ Hybridization (Dual CISH) Assay
title_short Evaluation of MYC Gene Amplification in Prostate Cancer Using a Dual Color Chromogenic In Situ Hybridization (Dual CISH) Assay
title_full Evaluation of MYC Gene Amplification in Prostate Cancer Using a Dual Color Chromogenic In Situ Hybridization (Dual CISH) Assay
title_fullStr Evaluation of MYC Gene Amplification in Prostate Cancer Using a Dual Color Chromogenic In Situ Hybridization (Dual CISH) Assay
title_full_unstemmed Evaluation of MYC Gene Amplification in Prostate Cancer Using a Dual Color Chromogenic In Situ Hybridization (Dual CISH) Assay
title_sort evaluation of myc gene amplification in prostate cancer using a dual color chromogenic in situ hybridization (dual cish) assay
publisher Publishing Company in Wilmington
publishDate 2013
url http://pa.bibdigital.ucc.edu.ar/1651/1/A_Lerda_Cabrera_Flores.pdf
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