Determinación de arbutina en las especies de Ilex
Arbutin (4-hydroxy-β-D-glucopyranoside) has important biological activities derived from its antioxidant, diuretic and urinary antiseptic properties. In this study, an HPLC method was used to identify and quantitate arbutin in several Ilex species. The analysed species were Ilex taubertiana Loes,...
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| Formato: | Tesis |
| Lenguaje: | Español |
| Publicado: |
Universidad de Belgrano. Facultad de Ciencias Exactas y Naturales. Farmacia
2015
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| Acceso en línea: | http://repositorio.ub.edu.ar/handle/123456789/5087 |
| Aporte de: |
| Sumario: | Arbutin (4-hydroxy-β-D-glucopyranoside) has important biological activities derived from its antioxidant,
diuretic and urinary antiseptic properties. In this study, an HPLC method was used to identify and quantitate
arbutin in several Ilex species. The analysed species were Ilex taubertiana Loes, Ilex integérrima
(Vellozo) Reissek, Ilex brevicuspis Reissek, Ilex pseudobuxus Reissek, Ilex argentina Lillo, Ilex brasiliensis
(Spreng) Loes, Ilex theezans Reissek, Ilex microdonta Reissek, Ilex dumosa var. dumosa Reissek, Ilex.
dumosa var. guaranina Loes, Ilex paraguariensis var. paraguariensis var. vestita and Ilex paraguariensis
var. paraguariensis St.-Hil.
Leaves of I. paraguariensis var. paraguariensis are used to produce Yerba Mate, a popular tea consumed
in Argentina, Uruguay, Paraguay and S. Brazil. Although cultivated, manufacturers often buy plant material
from other sources and this material can include other Ilex species that are used as adulterants due to the
similarity in their aspect (when dried and ground) and general chemical composition (caffeoylquinic acids,
saponins). Ilex paraguariensis, however, is the only species that contains xanthines (principally caffeine)
and is defined by the Food codex as the only one to be used to prepare Yerba mate. The presence of
arbutin could thus be a marker for the aduleteration by most of these cogeneric species.
The results obtained for each species were the following: I. taubertiana Loes 1.45% (P/P), I. integérrima
(Vellozo) Reissek 2.20% (P/P) , I. brevicuspis Reissek 2.42% (P/P), I. pseudobuxus Reissek 2.48% (P/P),
Ilex argentina Lillo 3.45% (P/P), I. brasiliensis (Spreng) Loes 5.35% (P/P), I. theezans Reissek 5.99%
(P/P) and I. microdonta Reissek 7.83% (P/P).
The analysis was performed using a system which consisted of a Kinetex C18 column and gradient
elution with two solvents: A= 1%HAc (in water); B= 1%Hac in methanol. Arbutin was quantitated at 281
nm wtith a PDA detector.Sample size was 20 µl.
The HPLC method was validated according to ICH guidelines: lineality, accuracy, precision, limit of
detection and specificity were determined. |
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