Determinación de arbutina en las especies de Ilex

Arbutin (4-hydroxy-β-D-glucopyranoside) has important biological activities derived from its antioxidant, diuretic and urinary antiseptic properties. In this study, an HPLC method was used to identify and quantitate arbutin in several Ilex species. The analysed species were Ilex taubertiana Loes,...

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Autor principal: Rafiee Seyed, Mohammed Ali
Otros Autores: Wilson, Erica
Formato: Tesis
Lenguaje:Español
Publicado: Universidad de Belgrano. Facultad de Ciencias Exactas y Naturales. Farmacia 2015
Materias:
Ciencias Exactas
Farmacia
Arbutina
Yerba mate
Exact Sciences
Pharmaceutics
Acceso en línea:http://repositorio.ub.edu.ar/handle/123456789/5087
Aporte de:
Repositorio Institucional - Universidad de Belgrano (UB) de Universidad de Belgrano
id I36-R142-123456789-5087
record_format dspace
institution Universidad de Belgrano
institution_str I-36
repository_str R-142
collection Repositorio Institucional - Universidad de Belgrano (UB)
language Español
topic Ciencias Exactas
Farmacia
Arbutina
Yerba mate
Exact Sciences
Pharmaceutics
Arbutina
Yerba mate
spellingShingle Ciencias Exactas
Farmacia
Arbutina
Yerba mate
Exact Sciences
Pharmaceutics
Arbutina
Yerba mate
Rafiee Seyed, Mohammed Ali
Determinación de arbutina en las especies de Ilex
topic_facet Ciencias Exactas
Farmacia
Arbutina
Yerba mate
Exact Sciences
Pharmaceutics
Arbutina
Yerba mate
description Arbutin (4-hydroxy-β-D-glucopyranoside) has important biological activities derived from its antioxidant, diuretic and urinary antiseptic properties. In this study, an HPLC method was used to identify and quantitate arbutin in several Ilex species. The analysed species were Ilex taubertiana Loes, Ilex integérrima (Vellozo) Reissek, Ilex brevicuspis Reissek, Ilex pseudobuxus Reissek, Ilex argentina Lillo, Ilex brasiliensis (Spreng) Loes, Ilex theezans Reissek, Ilex microdonta Reissek, Ilex dumosa var. dumosa Reissek, Ilex. dumosa var. guaranina Loes, Ilex paraguariensis var. paraguariensis var. vestita and Ilex paraguariensis var. paraguariensis St.-Hil. Leaves of I. paraguariensis var. paraguariensis are used to produce Yerba Mate, a popular tea consumed in Argentina, Uruguay, Paraguay and S. Brazil. Although cultivated, manufacturers often buy plant material from other sources and this material can include other Ilex species that are used as adulterants due to the similarity in their aspect (when dried and ground) and general chemical composition (caffeoylquinic acids, saponins). Ilex paraguariensis, however, is the only species that contains xanthines (principally caffeine) and is defined by the Food codex as the only one to be used to prepare Yerba mate. The presence of arbutin could thus be a marker for the aduleteration by most of these cogeneric species. The results obtained for each species were the following: I. taubertiana Loes 1.45% (P/P), I. integérrima (Vellozo) Reissek 2.20% (P/P) , I. brevicuspis Reissek 2.42% (P/P), I. pseudobuxus Reissek 2.48% (P/P), Ilex argentina Lillo 3.45% (P/P), I. brasiliensis (Spreng) Loes 5.35% (P/P), I. theezans Reissek 5.99% (P/P) and I. microdonta Reissek 7.83% (P/P). The analysis was performed using a system which consisted of a Kinetex C18 column and gradient elution with two solvents: A= 1%HAc (in water); B= 1%Hac in methanol. Arbutin was quantitated at 281 nm wtith a PDA detector.Sample size was 20 µl. The HPLC method was validated according to ICH guidelines: lineality, accuracy, precision, limit of detection and specificity were determined.
author2 Wilson, Erica
author_facet Wilson, Erica
Rafiee Seyed, Mohammed Ali
format Thesis
author Rafiee Seyed, Mohammed Ali
author_sort Rafiee Seyed, Mohammed Ali
title Determinación de arbutina en las especies de Ilex
title_short Determinación de arbutina en las especies de Ilex
title_full Determinación de arbutina en las especies de Ilex
title_fullStr Determinación de arbutina en las especies de Ilex
title_full_unstemmed Determinación de arbutina en las especies de Ilex
title_sort determinación de arbutina en las especies de ilex
publisher Universidad de Belgrano. Facultad de Ciencias Exactas y Naturales. Farmacia
publishDate 2015
url http://repositorio.ub.edu.ar/handle/123456789/5087
work_keys_str_mv AT rafieeseyedmohammedali determinaciondearbutinaenlasespeciesdeilex
bdutipo_str Repositorios
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