Radiation Chemical Studies of Methionine in Aqueous Solution: Understanding the Role of Molecular Oxygen

The oxidation of methionine is an important reaction in the biological milieu. Despite a few decades of intense studies, several fundamental aspects remain to be defined. We have investigated in detail the γ-radiolysis of free methionine in the absence and presence of molecular oxygen followed by...

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Autores principales: Barata-Vallejo, Sebastián, Ferreri, Carla, Postigo, Al, Chatgilialoglu, Chryssostomos
Formato: Artículo
Lenguaje:Inglés
Publicado: Universidad de Belgrano. Facultad de Ciencias Exactas y Naturales. Investigación 2015
Materias:
Ciencias exactas
Ingeniería
Química
Estudios químicos
Radiación
Metionina
Oxígeno molecular
Exact sciences
Engineering
Chemistry
Chemical studies
Radiation
Methionine
Molecular oxygen
Acceso en línea:http://repositorio.ub.edu.ar/handle/123456789/4843
Aporte de:
Repositorio Institucional - Universidad de Belgrano (UB) de Universidad de Belgrano
id I36-R142-123456789-4843
record_format dspace
institution Universidad de Belgrano
institution_str I-36
repository_str R-142
collection Repositorio Institucional - Universidad de Belgrano (UB)
language Inglés
topic Ciencias exactas
Ingeniería
Química
Estudios químicos
Radiación
Metionina
Oxígeno molecular
Exact sciences
Engineering
Chemistry
Chemical studies
Radiation
Methionine
Molecular oxygen
spellingShingle Ciencias exactas
Ingeniería
Química
Estudios químicos
Radiación
Metionina
Oxígeno molecular
Exact sciences
Engineering
Chemistry
Chemical studies
Radiation
Methionine
Molecular oxygen
Barata-Vallejo, Sebastián
Ferreri, Carla
Postigo, Al
Chatgilialoglu, Chryssostomos
Radiation Chemical Studies of Methionine in Aqueous Solution: Understanding the Role of Molecular Oxygen
topic_facet Ciencias exactas
Ingeniería
Química
Estudios químicos
Radiación
Metionina
Oxígeno molecular
Exact sciences
Engineering
Chemistry
Chemical studies
Radiation
Methionine
Molecular oxygen
description The oxidation of methionine is an important reaction in the biological milieu. Despite a few decades of intense studies, several fundamental aspects remain to be defined. We have investigated in detail the γ-radiolysis of free methionine in the absence and presence of molecular oxygen followed by product characterization and quantification. The primary site of attack by HO• radicals and H• atoms is the sulfur atom of methionine. We have disclosed that HO• radicals do not oxidize methionine to the corresponding sulfoxide in either the presence or the absence of oxygen; the oxidizing species is H2O2 derived either from the radiolysis of water or from the disproportionation of the byproduct O2 •-. 3-Methylthiopropionaldehyde is the major product of HO• radical attack in the presence of molecular oxygen. Together with the direct oxidation at sulfur as the major product, the potential of H• atoms is also proven to be highly specific for sulfur atom attack under anoxic and aerobic conditions. The major products derived from the H• atoms attack are found to be R-aminobutyric acid or homoserine, in the absence or presence of oxygen, respectively. All together, these results help clarify the fate of methionine related to a biological environment and offer a molecular basis for envisaging other possible pathways of in ViVo degradation as well as other markers.
format Article
author Barata-Vallejo, Sebastián
Ferreri, Carla
Postigo, Al
Chatgilialoglu, Chryssostomos
author_facet Barata-Vallejo, Sebastián
Ferreri, Carla
Postigo, Al
Chatgilialoglu, Chryssostomos
author_sort Barata-Vallejo, Sebastián
title Radiation Chemical Studies of Methionine in Aqueous Solution: Understanding the Role of Molecular Oxygen
title_short Radiation Chemical Studies of Methionine in Aqueous Solution: Understanding the Role of Molecular Oxygen
title_full Radiation Chemical Studies of Methionine in Aqueous Solution: Understanding the Role of Molecular Oxygen
title_fullStr Radiation Chemical Studies of Methionine in Aqueous Solution: Understanding the Role of Molecular Oxygen
title_full_unstemmed Radiation Chemical Studies of Methionine in Aqueous Solution: Understanding the Role of Molecular Oxygen
title_sort radiation chemical studies of methionine in aqueous solution: understanding the role of molecular oxygen
publisher Universidad de Belgrano. Facultad de Ciencias Exactas y Naturales. Investigación
publishDate 2015
url http://repositorio.ub.edu.ar/handle/123456789/4843
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AT postigoal radiationchemicalstudiesofmethionineinaqueoussolutionunderstandingtheroleofmolecularoxygen
AT chatgilialogluchryssostomos radiationchemicalstudiesofmethionineinaqueoussolutionunderstandingtheroleofmolecularoxygen
bdutipo_str Repositorios
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