Ischemic Conditioning Protects from Axoglial Alterations of the Optic Pathway Induced by Experimental Diabetes in Rats
Diabetic retinopathy is a leading cause of blindness. Visual function disorders have been demonstrated in diabetics even before the onset of retinopathy. At early stages of experimental diabetes, axoglial alterations occur at the distal portion of the optic nerve. Although ischemic conditioning ca...
Guardado en:
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Universidad de Belgrano - Facultad de Ciencias de la Salud - Proyectos de Investigación
2014
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| Materias: | |
| Acceso en línea: | http://repositorio.ub.edu.ar/handle/123456789/2741 |
| Aporte de: |
| Sumario: | Diabetic retinopathy is a leading cause of blindness. Visual function disorders have been demonstrated in diabetics even
before the onset of retinopathy. At early stages of experimental diabetes, axoglial alterations occur at the distal portion of
the optic nerve. Although ischemic conditioning can protect neurons and synaptic terminals against ischemic damage,
there is no information on its ability to protect axons. We analyzed the effect of ischemic conditioning on the early axoglial
alterations in the distal portion of the optic nerve induced by experimental diabetes. Diabetes was induced in Wistar rats by
an intraperitoneal injection of streptozotocin. Retinal ischemia was induced by increasing intraocular pressure to 120 mm
Hg for 5 min; this maneuver started 3 days after streptozotocin injection and was weekly repeated in one eye, while the
contralateral eye was submitted to a sham procedure. The application of ischemia pulses prevented a deficit in the
anterograde transport from the retina to the superior colliculus, as well as an increase in astrocyte reactivity, ultraestructural
myelin alterations, and altered morphology of oligodendrocyte lineage in the optic nerve distal portion at early stages of
experimental diabetes. Ischemia tolerance prevented a significant decrease of retinal glutamine synthetase activity induced
by diabetes. These results suggest that early vision loss in diabetes could be abated by ischemic conditioning which
preserved axonal function and structure. |
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