Palmitoylation of the feline immunodeficiency virus envelope glycoprotein and its effect on fusion activity and envelope incorporation into virions

The feline immunodeficiency virus (FIV) envelope glycoprotein (Env) possesses a short cytoplasmic domain of 53 amino acids containing four highly conserved cysteines at Env positions 804, 811, 815 and 848. Since palmitoylation of transmembrane proteins occurs at or near the membrane anchor, we in...

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Detalles Bibliográficos
Autores principales:	González, Silvia A., Paladino, Mónica G., Affranchino, José L.
Formato:	Artículo
Lenguaje:	Inglés
Publicado:	Universidad de Belgrano - Facultad de Ciencias Exactas y Naturales - Proyectos de Investigación 2014
Materias:	Feline immunodeficiency virus Envelope glycoprotein Click chemistry Fusogenic activity actividad fusogénica glicoproteína de la envoltura Virus de la inmunodeficiencia felina
Acceso en línea:	http://repositorio.ub.edu.ar/handle/123456789/2715
Aporte de:	Repositorio Institucional - Universidad de Belgrano (UB) de Universidad de Belgrano

id	I36-R142-123456789-2715
record_format	dspace
institution	Universidad de Belgrano
institution_str	I-36
repository_str	R-142
collection	Repositorio Institucional - Universidad de Belgrano (UB)
language	Inglés
topic	Feline immunodeficiency virus Envelope glycoprotein Click chemistry Fusogenic activity actividad fusogénica glicoproteína de la envoltura Virus de la inmunodeficiencia felina
spellingShingle	Feline immunodeficiency virus Envelope glycoprotein Click chemistry Fusogenic activity actividad fusogénica glicoproteína de la envoltura Virus de la inmunodeficiencia felina González, Silvia A. Paladino, Mónica G. Affranchino, José L. Palmitoylation of the feline immunodeficiency virus envelope glycoprotein and its effect on fusion activity and envelope incorporation into virions
topic_facet	Feline immunodeficiency virus Envelope glycoprotein Click chemistry Fusogenic activity actividad fusogénica glicoproteína de la envoltura Virus de la inmunodeficiencia felina
description	The feline immunodeficiency virus (FIV) envelope glycoprotein (Env) possesses a short cytoplasmic domain of 53 amino acids containing four highly conserved cysteines at Env positions 804, 811, 815 and 848. Since palmitoylation of transmembrane proteins occurs at or near the membrane anchor, we investigated whether cysteines 804, 811 and 815 are acylated and analyzed the relevance of these residues for Env functions. Replacement of cysteines 804, 811 and 815 individually or in combination by serine residues resulted in Env glycoproteins that were efficiently expressed and processed. However, mutations C804S and C811S reduced Env fusogenicity by 93% and 84%, respectively, compared with wild-type Env. By contrast, mutant C815S exhibited a fusogenic capacity representing 50% of the wild-type value. Remarkably, the double mutation C804S/C811S abrogated both Env fusion activity and Env incorporation into virions. Finally, by means of Click chemistry assays we demon-strated that the four FIV Env cytoplasmic cysteines are palmitoylated.
format	Article
author	González, Silvia A. Paladino, Mónica G. Affranchino, José L.
author_facet	González, Silvia A. Paladino, Mónica G. Affranchino, José L.
author_sort	González, Silvia A.
title	Palmitoylation of the feline immunodeficiency virus envelope glycoprotein and its effect on fusion activity and envelope incorporation into virions
title_short	Palmitoylation of the feline immunodeficiency virus envelope glycoprotein and its effect on fusion activity and envelope incorporation into virions
title_full	Palmitoylation of the feline immunodeficiency virus envelope glycoprotein and its effect on fusion activity and envelope incorporation into virions
title_fullStr	Palmitoylation of the feline immunodeficiency virus envelope glycoprotein and its effect on fusion activity and envelope incorporation into virions
title_full_unstemmed	Palmitoylation of the feline immunodeficiency virus envelope glycoprotein and its effect on fusion activity and envelope incorporation into virions
title_sort	palmitoylation of the feline immunodeficiency virus envelope glycoprotein and its effect on fusion activity and envelope incorporation into virions
publisher	Universidad de Belgrano - Facultad de Ciencias Exactas y Naturales - Proyectos de Investigación
publishDate	2014
url	http://repositorio.ub.edu.ar/handle/123456789/2715
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bdutipo_str	Repositorios
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Palmitoylation of the feline immunodeficiency virus envelope glycoprotein and its effect on fusion activity and envelope incorporation into virions

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