In vitro assembly of the feline immunodeficiency virus Gag polyprotein

The retroviral Gag protein is the only viral product that is necessary for the assembly of virions in mammalian cells. We have established an in vitro assembly system to study the assembly properties of purified feline immunodeficiency virus (FIV) Gag protein expressed in bacteria. Under fully defin...

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Autores principales: Affranchino, José L., González, Silvia A.
Formato: Artículo
Lenguaje:Inglés
Publicado: Universidad de Belgrano - Facultad de Ciencias Exactas y Naturales - Proyectos de Investigación 2014
Materias:
Gag polyprotein
Feline immunodeficiency virus
In vitro assembly
Lentiviruses
Los lentivirus
In vitro montaje
poliproteína Gag
virus de la inmunodeficiencia felina
Acceso en línea:http://repositorio.ub.edu.ar/handle/123456789/2711
Aporte de:
Repositorio Institucional - Universidad de Belgrano (UB) de Universidad de Belgrano
id I36-R142-123456789-2711
record_format dspace
institution Universidad de Belgrano
institution_str I-36
repository_str R-142
collection Repositorio Institucional - Universidad de Belgrano (UB)
language Inglés
topic Gag polyprotein
Feline immunodeficiency virus
In vitro assembly
Lentiviruses
Los lentivirus
In vitro montaje
poliproteína Gag
virus de la inmunodeficiencia felina
spellingShingle Gag polyprotein
Feline immunodeficiency virus
In vitro assembly
Lentiviruses
Los lentivirus
In vitro montaje
poliproteína Gag
virus de la inmunodeficiencia felina
Affranchino, José L.
González, Silvia A.
In vitro assembly of the feline immunodeficiency virus Gag polyprotein
topic_facet Gag polyprotein
Feline immunodeficiency virus
In vitro assembly
Lentiviruses
Los lentivirus
In vitro montaje
poliproteína Gag
virus de la inmunodeficiencia felina
description The retroviral Gag protein is the only viral product that is necessary for the assembly of virions in mammalian cells. We have established an in vitro assembly system to study the assembly properties of purified feline immunodeficiency virus (FIV) Gag protein expressed in bacteria. Under fully defined conditions, the FIV Gag protein assembles into spherical particles of 33 nm in diameter which are morphologically similar to authentic immature particles, albeit smaller than virions. The in vitro assembly of FIV Gag into particles was found to be resistant to the addition of Triton X-100 and required the presence of RNA. Notably, we found that an amino acid substitution in the nucleocapsid domain of Gag that impairs RNA binding and blocks virion production in vivo, also abrogates Gag assembly in vitro. The development of an in vitro assembly system for FIV Gag protein will facilitate the study of the mechanisms by which this protein assembles into immature particles.
format Article
author Affranchino, José L.
González, Silvia A.
author_facet Affranchino, José L.
González, Silvia A.
author_sort Affranchino, José L.
title In vitro assembly of the feline immunodeficiency virus Gag polyprotein
title_short In vitro assembly of the feline immunodeficiency virus Gag polyprotein
title_full In vitro assembly of the feline immunodeficiency virus Gag polyprotein
title_fullStr In vitro assembly of the feline immunodeficiency virus Gag polyprotein
title_full_unstemmed In vitro assembly of the feline immunodeficiency virus Gag polyprotein
title_sort in vitro assembly of the feline immunodeficiency virus gag polyprotein
publisher Universidad de Belgrano - Facultad de Ciencias Exactas y Naturales - Proyectos de Investigación
publishDate 2014
url http://repositorio.ub.edu.ar/handle/123456789/2711
work_keys_str_mv AT affranchinojosel invitroassemblyofthefelineimmunodeficiencyvirusgagpolyprotein
AT gonzalezsilviaa invitroassemblyofthefelineimmunodeficiencyvirusgagpolyprotein
bdutipo_str Repositorios
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