Importance of the short cytoplasmic domain of the feline immunodeficiency virus transmembrane glycoprotein for fusion activity and envelope glycoprotein incorporation into virions
The mature form of the envelope (Env) glycoprotein of lentiviruses is a heterodimer composed of the surface (SU) and transmembrane (TM) subunits. Feline immunodeficiency virus (FIV) possesses a TM glycoprotein with a cytoplasmic tail of approximately 53 amino acids which is unusually short compare...
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| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Universidad de Belgrano - Facultad de Ciencias Exactas y Naturales - Proyectos de Investigación
2014
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| Acceso en línea: | http://repositorio.ub.edu.ar/handle/123456789/2709 |
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I36-R142-123456789-2709 |
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dspace |
| institution |
Universidad de Belgrano |
| institution_str |
I-36 |
| repository_str |
R-142 |
| collection |
Repositorio Institucional - Universidad de Belgrano (UB) |
| language |
Inglés |
| topic |
Feline immunodeficiency virus Envelope glycoprotein Transmembrane glycoprotein Envelope cytoplasmic domain Fusogenic activity actividad fusogénica Envelope dominio citoplásmico glicoproteína transmembrana glicoproteína de la envoltura Virus de la inmunodeficiencia felina |
| spellingShingle |
Feline immunodeficiency virus Envelope glycoprotein Transmembrane glycoprotein Envelope cytoplasmic domain Fusogenic activity actividad fusogénica Envelope dominio citoplásmico glicoproteína transmembrana glicoproteína de la envoltura Virus de la inmunodeficiencia felina Celma, Cristina Paladino, Mónica G. González, Silvia A. Affranchino, José L. Importance of the short cytoplasmic domain of the feline immunodeficiency virus transmembrane glycoprotein for fusion activity and envelope glycoprotein incorporation into virions |
| topic_facet |
Feline immunodeficiency virus Envelope glycoprotein Transmembrane glycoprotein Envelope cytoplasmic domain Fusogenic activity actividad fusogénica Envelope dominio citoplásmico glicoproteína transmembrana glicoproteína de la envoltura Virus de la inmunodeficiencia felina |
| description |
The mature form of the envelope (Env) glycoprotein of lentiviruses is a heterodimer composed of the surface (SU) and transmembrane (TM)
subunits. Feline immunodeficiency virus (FIV) possesses a TM glycoprotein with a cytoplasmic tail of approximately 53 amino acids which is
unusually short compared with that of the other lentiviral glycoproteins (more than 100 residues). To investigate the relevance of the FIV TM
cytoplasmic domain to Env-mediated viral functions, we characterized the biological properties of a series of Env glycoproteins progressively
shortened from the carboxyl terminus. All the mutant Env proteins were efficiently expressed in feline cells and processed into the SU and TM
subunits. Deletion of 5 or 11 amino acids from the TM C-terminus did not significantly affect Env surface expression, fusogenic activity or Env
incorporation into virions, whereas removal of 17 or 23 residues impaired Env-mediated cell-to-cell fusion. Further truncation of the FIV TM by
29 residues resulted in an Env glycoprotein that was poorly expressed at the cell surface, exhibited only 20% of the wild-type Env fusogenic
capacity and was inefficiently incorporated into virions. Remarkably, deletion of the TM C-terminal 35 or 41 amino acids restored or even
enhanced Env biological functions. Indeed, these mutant Env glycoproteins bearing cytoplasmic domains of 18 or 12 amino acids were found to
be significantly more fusogenic than the wild-type Env and were efficiently incorporated into virions. Interestingly, truncation of the TM
cytoplasmic domain to only 6 amino acids did not affect Env incorporation into virions but abrogated Env fusogenicity. Finally, removal of the
entire TM cytoplasmic tail or deletion of as many as 6 amino acids into the membrane-spanning domain led to a complete loss of Env functions.
Our results demonstrate that despite its relatively short length, the FIV TM cytoplasmic domain plays an important role in modulating Env-mediated viral functions.
© 2007 Elsevier Inc. All rights reserved. |
| format |
Article |
| author |
Celma, Cristina Paladino, Mónica G. González, Silvia A. Affranchino, José L. |
| author_facet |
Celma, Cristina Paladino, Mónica G. González, Silvia A. Affranchino, José L. |
| author_sort |
Celma, Cristina |
| title |
Importance of the short cytoplasmic domain of the feline immunodeficiency virus transmembrane glycoprotein for fusion activity and envelope glycoprotein incorporation into virions |
| title_short |
Importance of the short cytoplasmic domain of the feline immunodeficiency virus transmembrane glycoprotein for fusion activity and envelope glycoprotein incorporation into virions |
| title_full |
Importance of the short cytoplasmic domain of the feline immunodeficiency virus transmembrane glycoprotein for fusion activity and envelope glycoprotein incorporation into virions |
| title_fullStr |
Importance of the short cytoplasmic domain of the feline immunodeficiency virus transmembrane glycoprotein for fusion activity and envelope glycoprotein incorporation into virions |
| title_full_unstemmed |
Importance of the short cytoplasmic domain of the feline immunodeficiency virus transmembrane glycoprotein for fusion activity and envelope glycoprotein incorporation into virions |
| title_sort |
importance of the short cytoplasmic domain of the feline immunodeficiency virus transmembrane glycoprotein for fusion activity and envelope glycoprotein incorporation into virions |
| publisher |
Universidad de Belgrano - Facultad de Ciencias Exactas y Naturales - Proyectos de Investigación |
| publishDate |
2014 |
| url |
http://repositorio.ub.edu.ar/handle/123456789/2709 |
| work_keys_str_mv |
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Repositorios |
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