Assay of caffeoylquinic acids in Baccharis trimera by reversed-phase liquid chromatography
Baccharis trimera commonly named ‘carqueja’, is wide-spread in South America and are used as raw material for herbal medicines. A reversed-phase liquid chromatography (RP-LC) method coupled to diode array detector was developed for the analysis of caffeoylquinic acids (CQAs), the main compounds resp...
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| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Universidad de Belgrano. Facultad de Ciencias Exactas y Naturales. Proyectos de Investigación
2014
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| Acceso en línea: | http://repositorio.ub.edu.ar/handle/123456789/2708 |
| Aporte de: |
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I36-R142-123456789-2708 |
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dspace |
| institution |
Universidad de Belgrano |
| institution_str |
I-36 |
| repository_str |
R-142 |
| collection |
Repositorio Institucional - Universidad de Belgrano (UB) |
| language |
Inglés |
| topic |
Baccharis trimera Caffeoylquinic acids Dicaffeoylquinic acids Tricaffeoylquinic acids RP-LC Photostability Fotoestabilidad |
| spellingShingle |
Baccharis trimera Caffeoylquinic acids Dicaffeoylquinic acids Tricaffeoylquinic acids RP-LC Photostability Fotoestabilidad Aboy, Ana Lúcia Anders Apel, Miriam Debenedetti, Sílvia Francescato, Leandro Rosella, María Adelaida Henriques, Amélia Teresinha Assay of caffeoylquinic acids in Baccharis trimera by reversed-phase liquid chromatography |
| topic_facet |
Baccharis trimera Caffeoylquinic acids Dicaffeoylquinic acids Tricaffeoylquinic acids RP-LC Photostability Fotoestabilidad |
| description |
Baccharis trimera commonly named ‘carqueja’, is wide-spread in South America and are used as raw material for herbal medicines. A reversed-phase liquid chromatography (RP-LC) method coupled to diode array detector was developed for the analysis of caffeoylquinic acids (CQAs), the main compounds responsible for its digestive activity. The identity of the quinic acids was established by mass spectrometry and were them: 5-O-[E]-caffeoylquinic acid, 3,4-O-[E]-dicaffeoylquinic acid, 3,5-O-[E]-dicaffeoylquinic acid, 4,5-O-[E]-dicaffeoylquinic acid and a tricaffeoylquinic acid. The RP-LC method for the quantitation of the caffeoylquinic acids was validated according to ICH guidelines, based on the following parameters: linearity, selectivity, robustness, limits of detection and quantification, precision and recovery. Hydroalcoholic extracts were prepared by the maceration of the plant material with ethanol:water 1:1 (v/v) in a 0.1:25 g mL−1 plant:solvent ratio in a water bath at 40 °C. Validation data indicated that the HPLC method proposed is suitable for the analysis of caffeoylquinic acids in B. trimera raw material. The results of the LOD and LOQ analyses for the 5-CQA were 4.1 μg mL−1 and 12.5 μg mL−1, respectively, 1.3 μg mL−1, 3.9 μg mL−1 for 4,5-diCQA and 1.7 μg mL−1, 5.1 μg mL−1 for triCQA. The levels of total CQAs ranged from 2.1 to 4.0 g% (w/w). The influence of season harvest and site collection was also evaluated and variations were observed in the results and can be related to phonologic phase, different locations, seasons and soil. Long term and photostability of plant material were carried out and was observed a stable behavior during the time of the experiments. |
| format |
Article |
| author |
Aboy, Ana Lúcia Anders Apel, Miriam Debenedetti, Sílvia Francescato, Leandro Rosella, María Adelaida Henriques, Amélia Teresinha |
| author_facet |
Aboy, Ana Lúcia Anders Apel, Miriam Debenedetti, Sílvia Francescato, Leandro Rosella, María Adelaida Henriques, Amélia Teresinha |
| author_sort |
Aboy, Ana Lúcia |
| title |
Assay of caffeoylquinic acids in Baccharis trimera by reversed-phase liquid chromatography |
| title_short |
Assay of caffeoylquinic acids in Baccharis trimera by reversed-phase liquid chromatography |
| title_full |
Assay of caffeoylquinic acids in Baccharis trimera by reversed-phase liquid chromatography |
| title_fullStr |
Assay of caffeoylquinic acids in Baccharis trimera by reversed-phase liquid chromatography |
| title_full_unstemmed |
Assay of caffeoylquinic acids in Baccharis trimera by reversed-phase liquid chromatography |
| title_sort |
assay of caffeoylquinic acids in baccharis trimera by reversed-phase liquid chromatography |
| publisher |
Universidad de Belgrano. Facultad de Ciencias Exactas y Naturales. Proyectos de Investigación |
| publishDate |
2014 |
| url |
http://repositorio.ub.edu.ar/handle/123456789/2708 |
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