In vitro binding of simian immunodeficiency virus matrix protein to the cytoplasmic domain of the envelope glycoprotein

Incorporation of the envelope (Env) glycoprotein into budding virions is a key step in the replication cycle of lentiviruses. Previously, we provided genetic and biochemical evidence indicating that Env packaging into simian immunodeficiency virus (SIV) particles is mediated by the association of t...

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Detalles Bibliográficos
Autores principales:	Manrique, Julieta M., Affranchino, José L., González, Silvia A.
Formato:	Artículo
Lenguaje:	Inglés
Publicado:	Universidad de Belgrano - Facultad de Ciencias Exactas y Naturales - Proyectos de Investigación 2014
Materias:	Simian immunodeficiency virus Virus de la inmunodeficiencia del simio Matrix protein Virus assembly Envelope glycoprotein cytoplasmic domain Glicoproteína de envoltura de dominio citoplásmico ensamblaje del virus proteína de la matriz
Acceso en línea:	http://repositorio.ub.edu.ar/handle/123456789/2707
Aporte de:	Repositorio Institucional - Universidad de Belgrano (UB) de Universidad de Belgrano

id	I36-R142-123456789-2707
record_format	dspace
institution	Universidad de Belgrano
institution_str	I-36
repository_str	R-142
collection	Repositorio Institucional - Universidad de Belgrano (UB)
language	Inglés
topic	Simian immunodeficiency virus Virus de la inmunodeficiencia del simio Matrix protein Virus assembly Envelope glycoprotein cytoplasmic domain Glicoproteína de envoltura de dominio citoplásmico ensamblaje del virus proteína de la matriz
spellingShingle	Simian immunodeficiency virus Virus de la inmunodeficiencia del simio Matrix protein Virus assembly Envelope glycoprotein cytoplasmic domain Glicoproteína de envoltura de dominio citoplásmico ensamblaje del virus proteína de la matriz Manrique, Julieta M. Affranchino, José L. González, Silvia A. In vitro binding of simian immunodeficiency virus matrix protein to the cytoplasmic domain of the envelope glycoprotein
topic_facet	Simian immunodeficiency virus Virus de la inmunodeficiencia del simio Matrix protein Virus assembly Envelope glycoprotein cytoplasmic domain Glicoproteína de envoltura de dominio citoplásmico ensamblaje del virus proteína de la matriz
description	Incorporation of the envelope (Env) glycoprotein into budding virions is a key step in the replication cycle of lentiviruses. Previously, we provided genetic and biochemical evidence indicating that Env packaging into simian immunodeficiency virus (SIV) particles is mediated by the association of the Env cytoplasmic domain (CD) with the matrix (MA) domain of Gag. In this study, we developed an in vitro binding assay that, based on recombinant proteins expressed in bacteria, allowed us to demonstrate the physical interaction between the SIV Env CD and the MA in the absence of other viral or cellular proteins. We show that this association is blocked by mutations in each of the interacting domains that have been reported to interfere in vivo with the incorporation of Env into SIV virions. Moreover, we determined that the binding of SIV MA to the Env CD is saturable with a dissociation constant of 7× 10−7 M. Interestingly, the SIV MA is capable of specifically interacting in vitro with the human immunodeficiency virus type 1 Env CD, but not with that of the distantly related feline immunodeficiency virus. Our results strongly support the notion that the association between the SIV MA and Env CD plays a central role in the process of SIV Env incorporation into Gag-made particles. © 2008 Elsevier Inc. All rights reserved.
format	Article
author	Manrique, Julieta M. Affranchino, José L. González, Silvia A.
author_facet	Manrique, Julieta M. Affranchino, José L. González, Silvia A.
author_sort	Manrique, Julieta M.
title	In vitro binding of simian immunodeficiency virus matrix protein to the cytoplasmic domain of the envelope glycoprotein
title_short	In vitro binding of simian immunodeficiency virus matrix protein to the cytoplasmic domain of the envelope glycoprotein
title_full	In vitro binding of simian immunodeficiency virus matrix protein to the cytoplasmic domain of the envelope glycoprotein
title_fullStr	In vitro binding of simian immunodeficiency virus matrix protein to the cytoplasmic domain of the envelope glycoprotein
title_full_unstemmed	In vitro binding of simian immunodeficiency virus matrix protein to the cytoplasmic domain of the envelope glycoprotein
title_sort	in vitro binding of simian immunodeficiency virus matrix protein to the cytoplasmic domain of the envelope glycoprotein
publisher	Universidad de Belgrano - Facultad de Ciencias Exactas y Naturales - Proyectos de Investigación
publishDate	2014
url	http://repositorio.ub.edu.ar/handle/123456789/2707
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bdutipo_str	Repositorios
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In vitro binding of simian immunodeficiency virus matrix protein to the cytoplasmic domain of the envelope glycoprotein

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