Mutations at the C-terminus of the simian immunodeficiency virus envelope glycoprotein affect gp120-gp41 stability on virions

The transmembrane (TM) subunit of the envelope (Env) glycoprotein of the simian immunodeficiency virus (SIV) contains an unusually long cytoplasmic domain of 164 amino acids. Previously, we identified domains in the SIV TM cytoplasmic tail that are necessary for Env incorporation into virions and...

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Autores principales: González, Silvia, Affranchino, José L.
Formato: Artículo
Lenguaje:Inglés
Publicado: Universidad de Belgrano - Facultad de Ciencias Exactas y Naturales - Proyectos de Investigación 2014
Materias:
Simian immunodeficiency virus
Virus de la inmunodeficiencia del simio
Envelope glycoprotein
Cytoplasmic domain
dominio citoplásmico
glicoproteína de la envoltura
Virus infectivity
infectividad del virus
Acceso en línea:http://repositorio.ub.edu.ar/handle/123456789/2705
Aporte de:
Repositorio Institucional - Universidad de Belgrano (UB) de Universidad de Belgrano
id I36-R142-123456789-2705
record_format dspace
institution Universidad de Belgrano
institution_str I-36
repository_str R-142
collection Repositorio Institucional - Universidad de Belgrano (UB)
language Inglés
topic Simian immunodeficiency virus
Virus de la inmunodeficiencia del simio
Envelope glycoprotein
Cytoplasmic domain
dominio citoplásmico
glicoproteína de la envoltura
Virus infectivity
infectividad del virus
spellingShingle Simian immunodeficiency virus
Virus de la inmunodeficiencia del simio
Envelope glycoprotein
Cytoplasmic domain
dominio citoplásmico
glicoproteína de la envoltura
Virus infectivity
infectividad del virus
González, Silvia
Affranchino, José L.
Mutations at the C-terminus of the simian immunodeficiency virus envelope glycoprotein affect gp120-gp41 stability on virions
topic_facet Simian immunodeficiency virus
Virus de la inmunodeficiencia del simio
Envelope glycoprotein
Cytoplasmic domain
dominio citoplásmico
glicoproteína de la envoltura
Virus infectivity
infectividad del virus
description The transmembrane (TM) subunit of the envelope (Env) glycoprotein of the simian immunodeficiency virus (SIV) contains an unusually long cytoplasmic domain of 164 amino acids. Previously, we identified domains in the SIV TM cytoplasmic tail that are necessary for Env incorporation into virions and viral infectivity. In this study, we investigated the relevance to Env function of the highly conserved sequence comprising the immediate C-terminal 19 residues of TM. To this end, small in-frame deletions as well as a premature stop codon mutation were introduced into the coding region for the SIV TM C-terminus. All the mutant Env glycoproteins were expressed, processed and transported to the cell surface in an essentially wild-type manner. Moreover, the ability of the mutant Env proteins to mediate cell-to-cell fusion was similar to or slightly lower than that of the wild-type Env. However, viruses expressing the mutant Env glycoproteins were found to be poorly infectious in single-cycle infectivity assays. Further characterization of the TM mutant viruses revealed that while exhibiting wild-type levels of the TM protein, they contained significantly lower levels of the Env surface (SU) subunit, which is consistent with increased SU shedding from virions after Env incorporation. This phenotype was independent of Gag processing, since genetic inactivation of the viral protease did not increase SU retention by the resulting immature particles. Our findings indicate that deletions at the C-terminus of the SIV Env promote the instability of the SU-TM association on the virion surface and point to an important role for the TM cytoplasmic domain in modulating Env structure. D 2005 Elsevier Inc. All rights reserved.
format Article
author González, Silvia
Affranchino, José L.
author_facet González, Silvia
Affranchino, José L.
author_sort González, Silvia
title Mutations at the C-terminus of the simian immunodeficiency virus envelope glycoprotein affect gp120-gp41 stability on virions
title_short Mutations at the C-terminus of the simian immunodeficiency virus envelope glycoprotein affect gp120-gp41 stability on virions
title_full Mutations at the C-terminus of the simian immunodeficiency virus envelope glycoprotein affect gp120-gp41 stability on virions
title_fullStr Mutations at the C-terminus of the simian immunodeficiency virus envelope glycoprotein affect gp120-gp41 stability on virions
title_full_unstemmed Mutations at the C-terminus of the simian immunodeficiency virus envelope glycoprotein affect gp120-gp41 stability on virions
title_sort mutations at the c-terminus of the simian immunodeficiency virus envelope glycoprotein affect gp120-gp41 stability on virions
publisher Universidad de Belgrano - Facultad de Ciencias Exactas y Naturales - Proyectos de Investigación
publishDate 2014
url http://repositorio.ub.edu.ar/handle/123456789/2705
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AT affranchinojosel mutationsatthecterminusofthesimianimmunodeficiencyvirusenvelopeglycoproteinaffectgp120gp41stabilityonvirions
bdutipo_str Repositorios
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