In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal

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Abstract: Heat transfer during cooling and warming is difficult to measure in cryo-devices; mathematical modelling is an alternative method that can describe these processes. In this study, we tested the validity of one such model by assessing in-vitro development of vitrified and warmed bovine oocy...

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Autores principales: Sansiñena, Marina Julia, Santos, María Victoria, Chirife, Jorge, Zaritzky, Noemí
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2020
Materias:
FERTILIZACION IN VITRO
TRANSFERENCIA DE CALOR
DESARROLLO EMBRIONARIO
CRIOPRESERVACION
VITRIFICACION
OVOCITOS
BOVINOS
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/9493
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-9493
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic FERTILIZACION IN VITRO
TRANSFERENCIA DE CALOR
DESARROLLO EMBRIONARIO
CRIOPRESERVACION
VITRIFICACION
OVOCITOS
BOVINOS
spellingShingle FERTILIZACION IN VITRO
TRANSFERENCIA DE CALOR
DESARROLLO EMBRIONARIO
CRIOPRESERVACION
VITRIFICACION
OVOCITOS
BOVINOS
Sansiñena, Marina Julia
Santos, María Victoria
Chirife, Jorge
Zaritzky, Noemí
In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
topic_facet FERTILIZACION IN VITRO
TRANSFERENCIA DE CALOR
DESARROLLO EMBRIONARIO
CRIOPRESERVACION
VITRIFICACION
OVOCITOS
BOVINOS
description Abstract: Heat transfer during cooling and warming is difficult to measure in cryo-devices; mathematical modelling is an alternative method that can describe these processes. In this study, we tested the validity of one such model by assessing in-vitro development of vitrified and warmed bovine oocytes after parthenogenetic activation and culture. The viability of oocytes vitrified in four different cryo-devices was assessed. Consistent with modelling predictions, oocytes vitrified using cryo-devices with the highest modelled cooling rates had significantly (P < 0.05) better cleavage and blastocyst formation rates. We then evaluated a two-step sample removal process, in which oocytes were held in nitrogen vapour for 15 s to simulate sample identification during clinical application, before being removed completely and warmed. Oocytes exposed to this procedure showed reduced developmental potential, according to the model, owing to thermodynamic instability and devitrification at relatively low temperatures. These findings suggest that cryo-device selection and handling, including method of removal from nitrogen storage, are critical to survival of vitrified oocytes. Limitations of the study include use of parthenogenetically activated rather than fertilized ova and lack of physical measurement of recrystallization. We suggest mathematical modelling could be used to predict the effect of critical steps in cryopreservation.
format Artículo
author Sansiñena, Marina Julia
Santos, María Victoria
Chirife, Jorge
Zaritzky, Noemí
author_facet Sansiñena, Marina Julia
Santos, María Victoria
Chirife, Jorge
Zaritzky, Noemí
author_sort Sansiñena, Marina Julia
title In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
title_short In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
title_full In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
title_fullStr In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
title_full_unstemmed In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
title_sort in-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
publisher Elsevier
publishDate 2020
url https://repositorio.uca.edu.ar/handle/123456789/9493
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bdutipo_str Repositorios
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