Larrea divaricata Cav. aqueous extract and nordihydroguariaretic acid modulate oxidative stress in submandibular glands of diabetic rats : a buccal protective in diabetes

Abstract: Background. Oxidative stress is an imbalance between the levels of reactive oxygen species (ROS), reactive nitrogen species (RNS) and endogenous antioxidants. The aetiology and pathogenesis of several oral diseases are attributed to this process. The antioxidant enzymes secreted in the sal...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Peralta, Ignacio, Marrassini, Carla, Barreiro Arcos, María Laura, Cremaschi, Graciela A., Anesini, Claudia
Formato: Artículo
Lenguaje:Inglés
Publicado: BMC 2019
Materias:
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/9301
Aporte de:
id I33-R139123456789-9301
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic ESTRES OXIDATIVO
ANTIOXIDANTES
ESTOMATOLOGIA
GLANDULAS SALIVALES
ENZIMAS
SALUD BUCAL
DIABETES
spellingShingle ESTRES OXIDATIVO
ANTIOXIDANTES
ESTOMATOLOGIA
GLANDULAS SALIVALES
ENZIMAS
SALUD BUCAL
DIABETES
Peralta, Ignacio
Marrassini, Carla
Barreiro Arcos, María Laura
Cremaschi, Graciela A.
Anesini, Claudia
Larrea divaricata Cav. aqueous extract and nordihydroguariaretic acid modulate oxidative stress in submandibular glands of diabetic rats : a buccal protective in diabetes
topic_facet ESTRES OXIDATIVO
ANTIOXIDANTES
ESTOMATOLOGIA
GLANDULAS SALIVALES
ENZIMAS
SALUD BUCAL
DIABETES
description Abstract: Background. Oxidative stress is an imbalance between the levels of reactive oxygen species (ROS), reactive nitrogen species (RNS) and endogenous antioxidants. The aetiology and pathogenesis of several oral diseases are attributed to this process. The antioxidant enzymes secreted in the saliva by submandibular glands maintain oral health through the scavenging of ROS. The objective of this work was to study the capacity of an aqueous extract of L. divaricata (AE), and its majority compound, nordihydroguariaretic acid (NDGA), to modulate the pro-oxidant/antioxidant status in submandibular glands in a model of oxidative stress induced by streptozotocin (STZ) in rats. Methods. To induce oxidative stress with STZ, a group of animals was treated i.p. with 1 X PBS (control group) and other group was injected i.p. once with STZ (60 mg/kg). Ten days after the treatment, blood samples were taken from the tail vain to determine the glucose levels. Animals with glucose values ≥300 mg/ml were selected. The submandibular glands of control and STZ treated animals were incubated with either the AE (500 μg/ml) or with NDGA (1.5 μg/ml), and the content of malondialdehyde (MDA), protein carbonyl groups, ROS and RNS, and the activity and expression of peroxidase (Px), superoxide dismutase (SOD) and inducible nitric oxide synthase (iNOS) were assayed. Results AE decreased the levels of MDA (P < 0.01) and protein carbonyl groups (P < 0.05), and modulated the levels of ROS such as hydrogen peroxide (H2O2)(P < 0.01), superoxide anion (O2.-) (P < 0.05) and nitric oxide (NO) (P < 0.05) in relation to the modulation of Px and iNOS expression. NDGA was found to be involved in these effects. Conclusions. The antioxidant activity of the AE in the submandibular glands would allow the maintenance of the antioxidant pool to prevent oral oxidative diseases.
format Artículo
author Peralta, Ignacio
Marrassini, Carla
Barreiro Arcos, María Laura
Cremaschi, Graciela A.
Anesini, Claudia
author_facet Peralta, Ignacio
Marrassini, Carla
Barreiro Arcos, María Laura
Cremaschi, Graciela A.
Anesini, Claudia
author_sort Peralta, Ignacio
title Larrea divaricata Cav. aqueous extract and nordihydroguariaretic acid modulate oxidative stress in submandibular glands of diabetic rats : a buccal protective in diabetes
title_short Larrea divaricata Cav. aqueous extract and nordihydroguariaretic acid modulate oxidative stress in submandibular glands of diabetic rats : a buccal protective in diabetes
title_full Larrea divaricata Cav. aqueous extract and nordihydroguariaretic acid modulate oxidative stress in submandibular glands of diabetic rats : a buccal protective in diabetes
title_fullStr Larrea divaricata Cav. aqueous extract and nordihydroguariaretic acid modulate oxidative stress in submandibular glands of diabetic rats : a buccal protective in diabetes
title_full_unstemmed Larrea divaricata Cav. aqueous extract and nordihydroguariaretic acid modulate oxidative stress in submandibular glands of diabetic rats : a buccal protective in diabetes
title_sort larrea divaricata cav. aqueous extract and nordihydroguariaretic acid modulate oxidative stress in submandibular glands of diabetic rats : a buccal protective in diabetes
publisher BMC
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/9301
work_keys_str_mv AT peraltaignacio larreadivaricatacavaqueousextractandnordihydroguariareticacidmodulateoxidativestressinsubmandibularglandsofdiabeticratsabuccalprotectiveindiabetes
AT marrassinicarla larreadivaricatacavaqueousextractandnordihydroguariareticacidmodulateoxidativestressinsubmandibularglandsofdiabeticratsabuccalprotectiveindiabetes
AT barreiroarcosmarialaura larreadivaricatacavaqueousextractandnordihydroguariareticacidmodulateoxidativestressinsubmandibularglandsofdiabeticratsabuccalprotectiveindiabetes
AT cremaschigracielaa larreadivaricatacavaqueousextractandnordihydroguariareticacidmodulateoxidativestressinsubmandibularglandsofdiabeticratsabuccalprotectiveindiabetes
AT anesiniclaudia larreadivaricatacavaqueousextractandnordihydroguariareticacidmodulateoxidativestressinsubmandibularglandsofdiabeticratsabuccalprotectiveindiabetes
bdutipo_str Repositorios
_version_ 1764820527820570625