The position of the double bond in monounsaturated free fatty acids is essential for the inhibition of the nicotinic acetylcholine receptor
Abstract: Free fatty acids (FFAs) are non-competitive antagonists of the nicotinic acetylcholine receptor (AChR). Their site of action is supposedly located at the lipid-AChR interface. To elucidate the mechanism involved in this antagonism, we studied the effect that FFAs with a single double-bond...
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| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier B.V.
2019
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| Acceso en línea: | https://repositorio.uca.edu.ar/handle/123456789/8767 |
| Aporte de: |
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I33-R139123456789-8767 |
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| record_format |
dspace |
| institution |
Universidad Católica Argentina |
| institution_str |
I-33 |
| repository_str |
R-139 |
| collection |
Repositorio Institucional de la Universidad Católica Argentina (UCA) |
| language |
Inglés |
| topic |
RECEPTORES PROTEINAS LIPIDOS COLESTEROL ESPECTROSCOPIA DE FLUORESCENCIA ELECTROFISIOLOGIA |
| spellingShingle |
RECEPTORES PROTEINAS LIPIDOS COLESTEROL ESPECTROSCOPIA DE FLUORESCENCIA ELECTROFISIOLOGIA Perillo, Vanesa L. Fernández-Nievas, Gaspar A. Vallés, Ana Sofía Barrantes, Francisco José Antollini, Silvia S. The position of the double bond in monounsaturated free fatty acids is essential for the inhibition of the nicotinic acetylcholine receptor |
| topic_facet |
RECEPTORES PROTEINAS LIPIDOS COLESTEROL ESPECTROSCOPIA DE FLUORESCENCIA ELECTROFISIOLOGIA |
| description |
Abstract: Free fatty acids (FFAs) are non-competitive antagonists of the nicotinic acetylcholine receptor (AChR). Their site of action is supposedly located at the lipid-AChR interface. To elucidate the mechanism involved in this antagonism, we studied the effect that FFAs with a single double-bond at different positions (ω6, ω9, ω11 and ω13 cis-18:1) have on different AChR properties. Electrophysiological studies showed that only two FFAs (ω6 and ω9) reduced the duration of the channel open-state. The briefest component of the closed-time distribution remained unaltered, suggesting that ω6 and ω9 behave as allosteric blockers. Fluorescence resonance energy transfer studies indicated that all FFAs locate at the lipid-AChR interface, ω6 being restricted to annular sites and all others occupying non-annular sites. The perturbation of the native membrane order by FFAs was evaluated by DPH (1,6-diphenyl-1,3,5-hexatriene) and Laurdan fluorescence polarization studies, with the greatest decrease observed for ω9 and ω11. AChR conformational changes produced by FFAs present at the lipid bilayer were evaluated by fluorescence quenching studies of pyrene-labeled AChR and also using the AChR conformational-sensitive probe crystal violet. All cis-FFAs produced AChR conformational changes at the transmembrane level, but only ω9, ω11 and ω13 perturbed the resting state. Thus, the position and isomerism of the torsion angle of unsaturated FFAs are probably a key factor in terms of AChR blockage, suggesting that FFAs with a unique cis double bond at a superficial position inside the membrane directly inhibit AChR function by perturbing a potential conserved core structure for AChR gating at that level. |
| format |
Artículo |
| author |
Perillo, Vanesa L. Fernández-Nievas, Gaspar A. Vallés, Ana Sofía Barrantes, Francisco José Antollini, Silvia S. |
| author_facet |
Perillo, Vanesa L. Fernández-Nievas, Gaspar A. Vallés, Ana Sofía Barrantes, Francisco José Antollini, Silvia S. |
| author_sort |
Perillo, Vanesa L. |
| title |
The position of the double bond in monounsaturated free fatty acids is essential for the inhibition of the nicotinic acetylcholine receptor |
| title_short |
The position of the double bond in monounsaturated free fatty acids is essential for the inhibition of the nicotinic acetylcholine receptor |
| title_full |
The position of the double bond in monounsaturated free fatty acids is essential for the inhibition of the nicotinic acetylcholine receptor |
| title_fullStr |
The position of the double bond in monounsaturated free fatty acids is essential for the inhibition of the nicotinic acetylcholine receptor |
| title_full_unstemmed |
The position of the double bond in monounsaturated free fatty acids is essential for the inhibition of the nicotinic acetylcholine receptor |
| title_sort |
position of the double bond in monounsaturated free fatty acids is essential for the inhibition of the nicotinic acetylcholine receptor |
| publisher |
Elsevier B.V. |
| publishDate |
2019 |
| url |
https://repositorio.uca.edu.ar/handle/123456789/8767 |
| work_keys_str_mv |
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Repositorios |
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