Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2

Abstract: Putative functions of the heterotrimeric G-protein subunit Gαi2-dependent signaling include ion channel regulation, cell differentiation, proliferation and apoptosis. Erythrocytes may, similar to apoptosis of nucleated cells, undergo eryptosis, characterized by cell shrinkage and cell memb...

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Autores principales: Bissinger, Rosi, Lang, Elisabeth, Ghashghaeinia, Mehrdad, Singh, Yogesh, Zelenak, Christine, Fehrenbacher, Birgit, Honisch, Sabina, Chen, Hong, Fakhri, Hajar, Umbach, Anja T., Liu, Guilai, Rexhepaj, Rexhep, Liu, Guoxing, Schaller, Martin, Mack, Andreas F., Lupescu, Adrian, Birnbaumer, Lutz, Lang, Florian, Qadri, Syed M.
Formato: Artículo
Lenguaje:Inglés
Publicado: Nature Research 2019
Materias:
APOPTOSIS
PROTEINA
BIOLOGIA CELULAR
CELULAS DE LA SANGRE
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/8762
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-8762
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic APOPTOSIS
PROTEINA
BIOLOGIA CELULAR
CELULAS DE LA SANGRE
spellingShingle APOPTOSIS
PROTEINA
BIOLOGIA CELULAR
CELULAS DE LA SANGRE
Bissinger, Rosi
Lang, Elisabeth
Ghashghaeinia, Mehrdad
Singh, Yogesh
Zelenak, Christine
Fehrenbacher, Birgit
Honisch, Sabina
Chen, Hong
Fakhri, Hajar
Umbach, Anja T.
Liu, Guilai
Rexhepaj, Rexhep
Liu, Guoxing
Schaller, Martin
Mack, Andreas F.
Lupescu, Adrian
Birnbaumer, Lutz
Lang, Florian
Qadri, Syed M.
Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2
topic_facet APOPTOSIS
PROTEINA
BIOLOGIA CELULAR
CELULAS DE LA SANGRE
description Abstract: Putative functions of the heterotrimeric G-protein subunit Gαi2-dependent signaling include ion channel regulation, cell differentiation, proliferation and apoptosis. Erythrocytes may, similar to apoptosis of nucleated cells, undergo eryptosis, characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine (PS) exposure. Eryptosis may be triggered by increased cytosolic Ca(2+) activity and ceramide. In the present study, we show that Gαi2 is expressed in both murine and human erythrocytes and further examined the survival of erythrocytes drawn from Gαi2-deficient mice (Gαi2(-/-)) and corresponding wild-type mice (Gαi2(+/+)). Our data show that plasma erythropoietin levels, erythrocyte maturation markers, erythrocyte counts, hematocrit and hemoglobin concentration were similar in Gαi2(-/-) and Gαi2(+/+) mice but the mean corpuscular volume was significantly larger in Gαi2(-/-) mice. Spontaneous PS exposure of circulating Gαi2(-/-) erythrocytes was significantly lower than that of circulating Gαi2(+/+) erythrocytes. PS exposure was significantly lower in Gαi2(-/-) than in Gαi2(+/+) erythrocytes following ex vivo exposure to hyperosmotic shock, bacterial sphingomyelinase or C6 ceramide. Erythrocyte Gαi2 deficiency further attenuated hyperosmotic shock-induced increase of cytosolic Ca(2+) activity and cell shrinkage. Moreover, Gαi2(-/-) erythrocytes were more resistant to osmosensitive hemolysis as compared to Gαi2(+/+) erythrocytes. In conclusion, Gαi2 deficiency in erythrocytes confers partial protection against suicidal cell death.
format Artículo
author Bissinger, Rosi
Lang, Elisabeth
Ghashghaeinia, Mehrdad
Singh, Yogesh
Zelenak, Christine
Fehrenbacher, Birgit
Honisch, Sabina
Chen, Hong
Fakhri, Hajar
Umbach, Anja T.
Liu, Guilai
Rexhepaj, Rexhep
Liu, Guoxing
Schaller, Martin
Mack, Andreas F.
Lupescu, Adrian
Birnbaumer, Lutz
Lang, Florian
Qadri, Syed M.
author_facet Bissinger, Rosi
Lang, Elisabeth
Ghashghaeinia, Mehrdad
Singh, Yogesh
Zelenak, Christine
Fehrenbacher, Birgit
Honisch, Sabina
Chen, Hong
Fakhri, Hajar
Umbach, Anja T.
Liu, Guilai
Rexhepaj, Rexhep
Liu, Guoxing
Schaller, Martin
Mack, Andreas F.
Lupescu, Adrian
Birnbaumer, Lutz
Lang, Florian
Qadri, Syed M.
author_sort Bissinger, Rosi
title Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2
title_short Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2
title_full Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2
title_fullStr Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2
title_full_unstemmed Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2
title_sort blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric g-protein subunit gαi2
publisher Nature Research
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/8762
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