Deep transcriptomic profiling of M1 macrophages lacking Trpc3

Abstract: In previous studies using mice with macrophage-specific loss of TRPC3 we found a significant, selective effect of TRPC3 on the biology of M1, or inflammatory macrophages. Whereas activation of some components of the unfolded protein response and the pro-apoptotic mediators CamkII and Stat1...

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Autores principales: Kumarasamy, Sivarajan, Solanki, Sumeet, Atolagbe, Oluwatomisin T., Joe, Bina, Birnbaumer, Lutz, Vazquez, Guillermo
Formato: Artículo
Lenguaje:Inglés
Publicado: Nature Research 2019
Materias:
SISTEMA INMUNOLOGICO
LIPIDOS
MARCADORES BIOLOGICOS
BIOLOGIA CELULAR
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/8737
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-8737
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic SISTEMA INMUNOLOGICO
LIPIDOS
MARCADORES BIOLOGICOS
BIOLOGIA CELULAR
spellingShingle SISTEMA INMUNOLOGICO
LIPIDOS
MARCADORES BIOLOGICOS
BIOLOGIA CELULAR
Kumarasamy, Sivarajan
Solanki, Sumeet
Atolagbe, Oluwatomisin T.
Joe, Bina
Birnbaumer, Lutz
Vazquez, Guillermo
Deep transcriptomic profiling of M1 macrophages lacking Trpc3
topic_facet SISTEMA INMUNOLOGICO
LIPIDOS
MARCADORES BIOLOGICOS
BIOLOGIA CELULAR
description Abstract: In previous studies using mice with macrophage-specific loss of TRPC3 we found a significant, selective effect of TRPC3 on the biology of M1, or inflammatory macrophages. Whereas activation of some components of the unfolded protein response and the pro-apoptotic mediators CamkII and Stat1 was impaired in Trpc3-deficient M1 cells, gathering insight about other molecular signatures within macrophages that might be affected by Trpc3 expression requires an alternative approach. In the present study we conducted RNA-seq analysis to interrogate the transcriptome of M1 macrophages derived from mice with macrophage-specific loss of TRPC3 and their littermate controls. We identified 160 significantly differentially expressed genes between the two groups, of which 62 were upregulated and 98 downregulated in control vs. Trpc3-deficient M1 macrophages. Gene ontology analysis revealed enrichment in processes associated to cellular movement and lipid signaling, whereas the enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways included networks for calcium signaling and cell adhesion molecules, among others. This is the first deep transcriptomic analysis of macrophages in the context of Trpc3 deficiency and the data presented constitutes a unique resource to further explore functions of TRPC3 in macrophage biology.
format Artículo
author Kumarasamy, Sivarajan
Solanki, Sumeet
Atolagbe, Oluwatomisin T.
Joe, Bina
Birnbaumer, Lutz
Vazquez, Guillermo
author_facet Kumarasamy, Sivarajan
Solanki, Sumeet
Atolagbe, Oluwatomisin T.
Joe, Bina
Birnbaumer, Lutz
Vazquez, Guillermo
author_sort Kumarasamy, Sivarajan
title Deep transcriptomic profiling of M1 macrophages lacking Trpc3
title_short Deep transcriptomic profiling of M1 macrophages lacking Trpc3
title_full Deep transcriptomic profiling of M1 macrophages lacking Trpc3
title_fullStr Deep transcriptomic profiling of M1 macrophages lacking Trpc3
title_full_unstemmed Deep transcriptomic profiling of M1 macrophages lacking Trpc3
title_sort deep transcriptomic profiling of m1 macrophages lacking trpc3
publisher Nature Research
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/8737
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AT joebina deeptranscriptomicprofilingofm1macrophageslackingtrpc3
AT birnbaumerlutz deeptranscriptomicprofilingofm1macrophageslackingtrpc3
AT vazquezguillermo deeptranscriptomicprofilingofm1macrophageslackingtrpc3
bdutipo_str Repositorios
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