Nicotinic α4 receptor-mediated cholinergic influences on food intake and activity patterns in hypothalamic circuits

Abstract: Nicotinic acetylcholine receptors (nAChRs) play an important role in regulating appetite and have been shown to do so by influencing neural activity in the hypothalamus. To shed light on the hypothalamic circuits governing acetylcholine's (ACh) regulation of appetite this study invest...

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Detalles Bibliográficos
Autores principales: García, Ana P., Aitta-aho, Teemu, Schaaf, Laura, Heeley, Nicholas, Heuschmid, Lena, Bai, Yunjing, Barrantes, Francisco José, Apergis-Schoute, John
Formato: Artículo
Lenguaje:Inglés
Publicado: Public Library of Science 2019
Materias:
MEDICINA
RECEPTORES
REGULACION DEL APETITO
HIPOTALAMO
NEURONAS
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/8735
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-8735
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic MEDICINA
RECEPTORES
REGULACION DEL APETITO
HIPOTALAMO
NEURONAS
spellingShingle MEDICINA
RECEPTORES
REGULACION DEL APETITO
HIPOTALAMO
NEURONAS
García, Ana P.
Aitta-aho, Teemu
Schaaf, Laura
Heeley, Nicholas
Heuschmid, Lena
Bai, Yunjing
Barrantes, Francisco José
Apergis-Schoute, John
Nicotinic α4 receptor-mediated cholinergic influences on food intake and activity patterns in hypothalamic circuits
topic_facet MEDICINA
RECEPTORES
REGULACION DEL APETITO
HIPOTALAMO
NEURONAS
description Abstract: Nicotinic acetylcholine receptors (nAChRs) play an important role in regulating appetite and have been shown to do so by influencing neural activity in the hypothalamus. To shed light on the hypothalamic circuits governing acetylcholine's (ACh) regulation of appetite this study investigated the influence of hypothalamic nAChRs expressing the α4 subunit. We found that antagonizing the α4β2 nAChR locally in the lateral hypothalamus with di-hydro-ß-erythroidine (DHβE), an α4 nAChR antagonist with moderate affinity, caused an increase in food intake following free access to food after a 12 hour fast, compared to saline-infused animals. Immunocytochemical analysis revealed that orexin/hypocretin (HO), oxytocin, and tyrosine hydroxylase (TH)-containing neurons in the A13 and A12 of the hypothalamus expressed the nAChR α4 subunit in varying amounts (34%, 42%, 50%, and 51%, respectively) whereas melanin concentrating hormone (MCH) neurons did not, suggesting that DHβE-mediated increases in food intake may be due to a direct activation of specific hypothalamic circuits. Systemic DHβE (2 mg/kg) administration similarly increased food intake following a 12 hour fast. In these animals a subpopulation of orexin/hypocretin neurons showed elevated activity compared to control animals and MCH neuronal activity was overall lower as measured by expression of the immediate early gene marker for neuronal activity cFos. However, oxytocin neurons in the paraventricular hypothalamus and TH-containing neurons in the A13 and A12 did not show differential activity patterns. These results indicate that various neurochemically distinct hypothalamic populations are under the influence of α4β2 nAChRs and that cholinergic inputs to the lateral hypothalamus can affect satiety signals through activation of local α4β2 nAChR-mediated transmission.
format Artículo
author García, Ana P.
Aitta-aho, Teemu
Schaaf, Laura
Heeley, Nicholas
Heuschmid, Lena
Bai, Yunjing
Barrantes, Francisco José
Apergis-Schoute, John
author_facet García, Ana P.
Aitta-aho, Teemu
Schaaf, Laura
Heeley, Nicholas
Heuschmid, Lena
Bai, Yunjing
Barrantes, Francisco José
Apergis-Schoute, John
author_sort García, Ana P.
title Nicotinic α4 receptor-mediated cholinergic influences on food intake and activity patterns in hypothalamic circuits
title_short Nicotinic α4 receptor-mediated cholinergic influences on food intake and activity patterns in hypothalamic circuits
title_full Nicotinic α4 receptor-mediated cholinergic influences on food intake and activity patterns in hypothalamic circuits
title_fullStr Nicotinic α4 receptor-mediated cholinergic influences on food intake and activity patterns in hypothalamic circuits
title_full_unstemmed Nicotinic α4 receptor-mediated cholinergic influences on food intake and activity patterns in hypothalamic circuits
title_sort nicotinic α4 receptor-mediated cholinergic influences on food intake and activity patterns in hypothalamic circuits
publisher Public Library of Science
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/8735
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