Resistance to inhibitors of cholinesterase 3 (Ric-3) expression promotes selective protein associations with the human α7-nicotinic acetylcholine receptor interactome
Abstract: The α7-nicotinic acetylcholine receptor (α7-nAChR) is a ligand-gated ion channel widely expressed in vertebrates and is associated with numerous physiological functions. As transmembrane ion channels, α7-nAChRs need to be expressed on the surface of the plasma membrane to function. The rec...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Public Library of Science
2019
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| Acceso en línea: | https://repositorio.uca.edu.ar/handle/123456789/8728 |
| Aporte de: |
| id |
I33-R139123456789-8728 |
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| record_format |
dspace |
| institution |
Universidad Católica Argentina |
| institution_str |
I-33 |
| repository_str |
R-139 |
| collection |
Repositorio Institucional de la Universidad Católica Argentina (UCA) |
| language |
Inglés |
| topic |
MEDICINA CANALES IONICOS RECEPTORES MEMBRANAS CELULARES PROTEINAS |
| spellingShingle |
MEDICINA CANALES IONICOS RECEPTORES MEMBRANAS CELULARES PROTEINAS Mulcahy, Matthew J. Blattman, Sydney B. Barrantes, Francisco José Lukas, Ronald J. Hawrot, Edward Resistance to inhibitors of cholinesterase 3 (Ric-3) expression promotes selective protein associations with the human α7-nicotinic acetylcholine receptor interactome |
| topic_facet |
MEDICINA CANALES IONICOS RECEPTORES MEMBRANAS CELULARES PROTEINAS |
| description |
Abstract: The α7-nicotinic acetylcholine receptor (α7-nAChR) is a ligand-gated ion channel widely expressed in vertebrates and is associated with numerous physiological functions. As transmembrane ion channels, α7-nAChRs need to be expressed on the surface of the plasma membrane to function. The receptor has been reported to associate with proteins involved with receptor biogenesis, modulation of receptor properties, as well as intracellular signaling cascades and some of these associated proteins may affect surface expression of α7-nAChRs. The putative chaperone resistance to inhibitors of cholinesterase 3 (Ric-3) has been reported to interact with, and enhance the surface expression of, α7-nAChRs. In this study, we identified proteins that associate with α7-nAChRs when Ric-3 is expressed. Using α-bungarotoxin (α-bgtx), we isolated and compared α7-nAChR-associated proteins from two stably transfected, human tumor-derived cell lines: SH-EP1-hα7 expressing human α7-nAChRs and the same cell line further transfected to express Ric-3, SH-EP1-hα7-Ric-3. Mass spectrometric analysis of peptides identified thirty-nine proteins that are associated with α7-nAChRs only when Ric-3 was expressed. Significantly, and consistent with reports of Ric-3 function in the literature, several of the identified proteins are involved in biological processes that may affect nAChR surface expression such as post-translational processing of proteins, protein trafficking, and protein transport. Additionally, proteins affecting the cell cycle, the cytoskeleton, stress responses, as well as cyclic AMP- and inositol triphosphate-dependent signaling cascades were identified. These results illuminate how α-bgtx may be used to isolate and identify α7-nAChRs as well as how the expression of chaperones such as Ric-3 can influence proteins associating with α7-nAChRs. These associating proteins may alter activities of α7-nAChRs to expand their functionally-relevant repertoire as well as to affect biogenesis and membrane trafficking of α7-nAChRs. |
| format |
Artículo |
| author |
Mulcahy, Matthew J. Blattman, Sydney B. Barrantes, Francisco José Lukas, Ronald J. Hawrot, Edward |
| author_facet |
Mulcahy, Matthew J. Blattman, Sydney B. Barrantes, Francisco José Lukas, Ronald J. Hawrot, Edward |
| author_sort |
Mulcahy, Matthew J. |
| title |
Resistance to inhibitors of cholinesterase 3 (Ric-3) expression promotes selective protein associations with the human α7-nicotinic acetylcholine receptor interactome |
| title_short |
Resistance to inhibitors of cholinesterase 3 (Ric-3) expression promotes selective protein associations with the human α7-nicotinic acetylcholine receptor interactome |
| title_full |
Resistance to inhibitors of cholinesterase 3 (Ric-3) expression promotes selective protein associations with the human α7-nicotinic acetylcholine receptor interactome |
| title_fullStr |
Resistance to inhibitors of cholinesterase 3 (Ric-3) expression promotes selective protein associations with the human α7-nicotinic acetylcholine receptor interactome |
| title_full_unstemmed |
Resistance to inhibitors of cholinesterase 3 (Ric-3) expression promotes selective protein associations with the human α7-nicotinic acetylcholine receptor interactome |
| title_sort |
resistance to inhibitors of cholinesterase 3 (ric-3) expression promotes selective protein associations with the human α7-nicotinic acetylcholine receptor interactome |
| publisher |
Public Library of Science |
| publishDate |
2019 |
| url |
https://repositorio.uca.edu.ar/handle/123456789/8728 |
| work_keys_str_mv |
AT mulcahymatthewj resistancetoinhibitorsofcholinesterase3ric3expressionpromotesselectiveproteinassociationswiththehumana7nicotinicacetylcholinereceptorinteractome AT blattmansydneyb resistancetoinhibitorsofcholinesterase3ric3expressionpromotesselectiveproteinassociationswiththehumana7nicotinicacetylcholinereceptorinteractome AT barrantesfranciscojose resistancetoinhibitorsofcholinesterase3ric3expressionpromotesselectiveproteinassociationswiththehumana7nicotinicacetylcholinereceptorinteractome AT lukasronaldj resistancetoinhibitorsofcholinesterase3ric3expressionpromotesselectiveproteinassociationswiththehumana7nicotinicacetylcholinereceptorinteractome AT hawrotedward resistancetoinhibitorsofcholinesterase3ric3expressionpromotesselectiveproteinassociationswiththehumana7nicotinicacetylcholinereceptorinteractome |
| bdutipo_str |
Repositorios |
| _version_ |
1764820528484319233 |