Lipid-independent control of endothelial and neuronal TRPC3 channels by light

Abstract: Lipid-gated TRPC channels are highly expressed in cardiovascular and neuronal tissues. Exerting precise pharmacological control over their activity in native cells is expected to serve as a basis for the development of novel therapies. Here we report on a new photopharmacological tool that...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Tiapko, Oleksandra, Shrestha, Niroj, Lindinger, Sonja, Guedes de la Cruz, Gema, Graziani, Annarita, Klec, Christiane, Butorac, Carmen, Graier, Wolfgang F., Kubista, Helmut, Freichel, Marc, Birnbaumer, Lutz, Romanin, Christoph, Glasnov, Toma, Groschner, Klaus
Formato: Artículo
Lenguaje:Inglés
Publicado: Royal Society of Chemistry 2019
Materias:
LIPIDOS
FARMACOLOGIA
FISIOLOGIA
CELULAS
LUZ
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/8681
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-8681
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic LIPIDOS
FARMACOLOGIA
FISIOLOGIA
CELULAS
LUZ
spellingShingle LIPIDOS
FARMACOLOGIA
FISIOLOGIA
CELULAS
LUZ
Tiapko, Oleksandra
Shrestha, Niroj
Lindinger, Sonja
Guedes de la Cruz, Gema
Graziani, Annarita
Klec, Christiane
Butorac, Carmen
Graier, Wolfgang F.
Kubista, Helmut
Freichel, Marc
Birnbaumer, Lutz
Romanin, Christoph
Glasnov, Toma
Groschner, Klaus
Lipid-independent control of endothelial and neuronal TRPC3 channels by light
topic_facet LIPIDOS
FARMACOLOGIA
FISIOLOGIA
CELULAS
LUZ
description Abstract: Lipid-gated TRPC channels are highly expressed in cardiovascular and neuronal tissues. Exerting precise pharmacological control over their activity in native cells is expected to serve as a basis for the development of novel therapies. Here we report on a new photopharmacological tool that enables manipulation of TRPC3 channels by light, in a manner independent of lipid metabolism and with higher temporal precision than lipid photopharmacology. Using the azobenzene photoswitch moiety, we modified GSK1702934A to generate light-controlled TRPC agonists. We obtained one light-sensitive molecule (OptoBI-1) that allows us to exert efficient, light-mediated control over TRPC3 activity and the associated cellular Ca2+ signaling. OptoBI-1 enabled high-precision, temporal control of TRPC3-linked cell functions such as neuronal firing and endothelial Ca2+ transients. With these findings, we introduce a novel photopharmacological strategy to control native TRPC conductances.
format Artículo
author Tiapko, Oleksandra
Shrestha, Niroj
Lindinger, Sonja
Guedes de la Cruz, Gema
Graziani, Annarita
Klec, Christiane
Butorac, Carmen
Graier, Wolfgang F.
Kubista, Helmut
Freichel, Marc
Birnbaumer, Lutz
Romanin, Christoph
Glasnov, Toma
Groschner, Klaus
author_facet Tiapko, Oleksandra
Shrestha, Niroj
Lindinger, Sonja
Guedes de la Cruz, Gema
Graziani, Annarita
Klec, Christiane
Butorac, Carmen
Graier, Wolfgang F.
Kubista, Helmut
Freichel, Marc
Birnbaumer, Lutz
Romanin, Christoph
Glasnov, Toma
Groschner, Klaus
author_sort Tiapko, Oleksandra
title Lipid-independent control of endothelial and neuronal TRPC3 channels by light
title_short Lipid-independent control of endothelial and neuronal TRPC3 channels by light
title_full Lipid-independent control of endothelial and neuronal TRPC3 channels by light
title_fullStr Lipid-independent control of endothelial and neuronal TRPC3 channels by light
title_full_unstemmed Lipid-independent control of endothelial and neuronal TRPC3 channels by light
title_sort lipid-independent control of endothelial and neuronal trpc3 channels by light
publisher Royal Society of Chemistry
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/8681
work_keys_str_mv AT tiapkooleksandra lipidindependentcontrolofendothelialandneuronaltrpc3channelsbylight
AT shresthaniroj lipidindependentcontrolofendothelialandneuronaltrpc3channelsbylight
AT lindingersonja lipidindependentcontrolofendothelialandneuronaltrpc3channelsbylight
AT guedesdelacruzgema lipidindependentcontrolofendothelialandneuronaltrpc3channelsbylight
AT grazianiannarita lipidindependentcontrolofendothelialandneuronaltrpc3channelsbylight
AT klecchristiane lipidindependentcontrolofendothelialandneuronaltrpc3channelsbylight
AT butoraccarmen lipidindependentcontrolofendothelialandneuronaltrpc3channelsbylight
AT graierwolfgangf lipidindependentcontrolofendothelialandneuronaltrpc3channelsbylight
AT kubistahelmut lipidindependentcontrolofendothelialandneuronaltrpc3channelsbylight
AT freichelmarc lipidindependentcontrolofendothelialandneuronaltrpc3channelsbylight
AT birnbaumerlutz lipidindependentcontrolofendothelialandneuronaltrpc3channelsbylight
AT romaninchristoph lipidindependentcontrolofendothelialandneuronaltrpc3channelsbylight
AT glasnovtoma lipidindependentcontrolofendothelialandneuronaltrpc3channelsbylight
AT groschnerklaus lipidindependentcontrolofendothelialandneuronaltrpc3channelsbylight
bdutipo_str Repositorios
_version_ 1764820528422453248

Ejemplares similares