Transient receptor potential canonical 3 (TRPC3) channels are required for hypothalamic glucose detection and energy homeostasis
The mediobasal hypothalamus (MBH) contains neurons capable of directly detecting metabolic signals such as glucose to control energy homeostasis. Among them, glucose-excited (GE) neurons increase their electrical activity when glucose rises. In view of previous work, we hypothesized that transient r...
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| Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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American Diabetes Association
2019
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| Acceso en línea: | https://repositorio.uca.edu.ar/handle/123456789/8679 |
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I33-R139123456789-8679 |
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| record_format |
dspace |
| institution |
Universidad Católica Argentina |
| institution_str |
I-33 |
| repository_str |
R-139 |
| collection |
Repositorio Institucional de la Universidad Católica Argentina (UCA) |
| language |
Inglés |
| topic |
GLUCOSA HIPOTALAMICA HOMEOSTASIS ENERGETICA TRPC3 CANALES IONICOS |
| spellingShingle |
GLUCOSA HIPOTALAMICA HOMEOSTASIS ENERGETICA TRPC3 CANALES IONICOS Chrétien, Chloé Fenech, Claire Liénard, Fabienne Grall, Sylvie Chevalier, Charlène Chaudy, Sylvie Brenachot, Xavier Berges, Raymond Louche, Katie Stark, Romana Nédélec, Emmanuelle Laderrière, Amélie Andrews, Zane B. Benani, Alexandre Flockerzi, Veit Gascuel, Jean Hartmann, Jana Moro, Cédric Birnbaumer, Lutz Leloup, Corinne Pénicaud, Luc Fioramonti, Xavier Transient receptor potential canonical 3 (TRPC3) channels are required for hypothalamic glucose detection and energy homeostasis |
| topic_facet |
GLUCOSA HIPOTALAMICA HOMEOSTASIS ENERGETICA TRPC3 CANALES IONICOS |
| description |
The mediobasal hypothalamus (MBH) contains neurons capable of directly detecting metabolic signals such as glucose to control energy homeostasis. Among them, glucose-excited (GE) neurons increase their electrical activity when glucose rises. In view of previous work, we hypothesized that transient receptor potential canonical type 3 (TRPC3) channels are involved in hypothalamic glucose detection and the control of energy homeostasis. To investigate the role of TRPC3, we used constitutive and conditional TRPC3-deficient mouse models. Hypothalamic glucose detection was studied in vivo by measuring food intake and insulin secretion in response to increased brain glucose level. The role of TRPC3 in GE neuron response to glucose was studied by using in vitro calcium imaging on freshly dissociated MBH neurons. We found that whole-body and MBH TRPC3-deficient mice have increased body weight and food intake. The anorectic effect of intracerebroventricular glucose and the insulin secretory response to intracarotid glucose injection are blunted in TRPC3-deficient mice. TRPC3 loss of function or pharmacological inhibition blunts calcium responses to glucose in MBH neurons in vitro. Together, the results demonstrate that TRPC3 channels are required for the response to glucose of MBH GE neurons and the central effect of glucose on insulin secretion and food intake. |
| format |
Artículo |
| author |
Chrétien, Chloé Fenech, Claire Liénard, Fabienne Grall, Sylvie Chevalier, Charlène Chaudy, Sylvie Brenachot, Xavier Berges, Raymond Louche, Katie Stark, Romana Nédélec, Emmanuelle Laderrière, Amélie Andrews, Zane B. Benani, Alexandre Flockerzi, Veit Gascuel, Jean Hartmann, Jana Moro, Cédric Birnbaumer, Lutz Leloup, Corinne Pénicaud, Luc Fioramonti, Xavier |
| author_facet |
Chrétien, Chloé Fenech, Claire Liénard, Fabienne Grall, Sylvie Chevalier, Charlène Chaudy, Sylvie Brenachot, Xavier Berges, Raymond Louche, Katie Stark, Romana Nédélec, Emmanuelle Laderrière, Amélie Andrews, Zane B. Benani, Alexandre Flockerzi, Veit Gascuel, Jean Hartmann, Jana Moro, Cédric Birnbaumer, Lutz Leloup, Corinne Pénicaud, Luc Fioramonti, Xavier |
| author_sort |
Chrétien, Chloé |
| title |
Transient receptor potential canonical 3 (TRPC3) channels are required for hypothalamic glucose detection and energy homeostasis |
| title_short |
Transient receptor potential canonical 3 (TRPC3) channels are required for hypothalamic glucose detection and energy homeostasis |
| title_full |
Transient receptor potential canonical 3 (TRPC3) channels are required for hypothalamic glucose detection and energy homeostasis |
| title_fullStr |
Transient receptor potential canonical 3 (TRPC3) channels are required for hypothalamic glucose detection and energy homeostasis |
| title_full_unstemmed |
Transient receptor potential canonical 3 (TRPC3) channels are required for hypothalamic glucose detection and energy homeostasis |
| title_sort |
transient receptor potential canonical 3 (trpc3) channels are required for hypothalamic glucose detection and energy homeostasis |
| publisher |
American Diabetes Association |
| publishDate |
2019 |
| url |
https://repositorio.uca.edu.ar/handle/123456789/8679 |
| work_keys_str_mv |
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Repositorios |
| _version_ |
1764820528419307522 |