Oxidative stress produced by hyperthyroidism status induces the antioxidant enzyme transcription through the activation of the nrf-2 factor in lymphoid tissues of balb/c mice

Abstract: Hyperthyroidism is an endocrine disorder characterized by excessive secretion of thyroid hormones T3 and T4. Thyroid hormones exert pleiotropic actions on numerous tissues and induce an overall increase in metabolism, with an increase in energy demand and oxygen consumption. Therefore, the...

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Autores principales: Costilla, Melisa, Macri Delbono, Rodrigo, Klecha, Alicia Juana, Cremaschi, Graciela A., Barreiro Arcos, María Laura
Otros Autores: 0000-0001-8078-7162
Formato: Artículo
Lenguaje:Inglés
Publicado: Hindawi 2019
Materias:
HIPERTIROIDISMO
GANGLIOS LINFATICOS
ESTRES OXIDATIVO
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/8673
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-8673
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic HIPERTIROIDISMO
GANGLIOS LINFATICOS
ESTRES OXIDATIVO
spellingShingle HIPERTIROIDISMO
GANGLIOS LINFATICOS
ESTRES OXIDATIVO
Costilla, Melisa
Macri Delbono, Rodrigo
Klecha, Alicia Juana
Cremaschi, Graciela A.
Barreiro Arcos, María Laura
Oxidative stress produced by hyperthyroidism status induces the antioxidant enzyme transcription through the activation of the nrf-2 factor in lymphoid tissues of balb/c mice
topic_facet HIPERTIROIDISMO
GANGLIOS LINFATICOS
ESTRES OXIDATIVO
description Abstract: Hyperthyroidism is an endocrine disorder characterized by excessive secretion of thyroid hormones T3 and T4. Thyroid hormones exert pleiotropic actions on numerous tissues and induce an overall increase in metabolism, with an increase in energy demand and oxygen consumption. Therefore, the purpose of this study was to investigate the effects of hyperthyroidism on the production of reactive oxygen species (ROS) in lymph node and spleen cells of euthyroid and hyperthyroid mice, analyzing antioxidant mechanisms involved in the restitution of the cellular redox state. For this, thirty female Balb/c (H-2d) mice were randomly divided into two groups: euthyroid (by treatment with placebo) and hyperthyroid (by treatment with 12 mg/l of T4 in drinking water for 30 days). We found a significant increase in ROS and an increase in the genomic and protein expression of the antioxidant enzymes catalase (CAT) and glutathione peroxidase-1 (GPx-1) in lymph node and spleen cells of hyperthyroid mice. In vitro treatment with H2O2 (250 μM) of the lymphoid cells of euthyroid mice increased the expression levels of CAT and GPx-1. The hyperthyroidism increased the phosphorylation levels of Nrf2 (nuclear factor erythroid 2-related factor) and the kinase activity of protein kinase C (PKC) and extracellular signal-regulated kinase (ERK). Additionally, we found an increase in the expression of the classic isoenzymes of PKCα, β and γ. In conclusion, these results indicated that the increase in ROS found in the hyperthyroid state induces the antioxidant enzyme transcription through the activation of the Nrf-2 factor in lymphoid tissues. This shows the influence of hyperthyroidism on the regulation of the cellular antioxidant system.
author2 0000-0001-8078-7162
author_facet 0000-0001-8078-7162
Costilla, Melisa
Macri Delbono, Rodrigo
Klecha, Alicia Juana
Cremaschi, Graciela A.
Barreiro Arcos, María Laura
format Artículo
author Costilla, Melisa
Macri Delbono, Rodrigo
Klecha, Alicia Juana
Cremaschi, Graciela A.
Barreiro Arcos, María Laura
author_sort Costilla, Melisa
title Oxidative stress produced by hyperthyroidism status induces the antioxidant enzyme transcription through the activation of the nrf-2 factor in lymphoid tissues of balb/c mice
title_short Oxidative stress produced by hyperthyroidism status induces the antioxidant enzyme transcription through the activation of the nrf-2 factor in lymphoid tissues of balb/c mice
title_full Oxidative stress produced by hyperthyroidism status induces the antioxidant enzyme transcription through the activation of the nrf-2 factor in lymphoid tissues of balb/c mice
title_fullStr Oxidative stress produced by hyperthyroidism status induces the antioxidant enzyme transcription through the activation of the nrf-2 factor in lymphoid tissues of balb/c mice
title_full_unstemmed Oxidative stress produced by hyperthyroidism status induces the antioxidant enzyme transcription through the activation of the nrf-2 factor in lymphoid tissues of balb/c mice
title_sort oxidative stress produced by hyperthyroidism status induces the antioxidant enzyme transcription through the activation of the nrf-2 factor in lymphoid tissues of balb/c mice
publisher Hindawi
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/8673
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