Sab is differentially expressed in the brain and affects neuronal activity

Abstract: Sab (SH3 binding protein 5 or SH3BP5) is a mitochondrial scaffold protein involved in signaling associated with mitochondrial dysfunction and apoptosis; furthermore, Sab is a crucial signaling platform for neurodegenerative disease. To determine how this signaling nexus could have a signif...

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Detalles Bibliográficos
Autores principales: Sodero, Alejandro Omar, Rodriguez-Silva, Mónica, Salio, Chiara, Sassoè-Pognetto, Marco, Chambers, Jeremy W.
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2019
Materias:
PROTEINAS
QUINASA
ACTIVIDAD NEURONAL
CEREBRO
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/8567
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-8567
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic PROTEINAS
QUINASA
ACTIVIDAD NEURONAL
CEREBRO
spellingShingle PROTEINAS
QUINASA
ACTIVIDAD NEURONAL
CEREBRO
Sodero, Alejandro Omar
Rodriguez-Silva, Mónica
Salio, Chiara
Sassoè-Pognetto, Marco
Chambers, Jeremy W.
Sab is differentially expressed in the brain and affects neuronal activity
topic_facet PROTEINAS
QUINASA
ACTIVIDAD NEURONAL
CEREBRO
description Abstract: Sab (SH3 binding protein 5 or SH3BP5) is a mitochondrial scaffold protein involved in signaling associated with mitochondrial dysfunction and apoptosis; furthermore, Sab is a crucial signaling platform for neurodegenerative disease. To determine how this signaling nexus could have a significant effect on disease, we examined the regional abundance of Sab in the brain and sub-neuronal distribution, and we monitored the effect of Sab-mediated signaling on neuronal activity. We found that Sab is widely expressed in the adult mouse brain with increased abundance in hippocampus, ventral midbrain, and cerebellum. Sab was found in purified synaptosomes and in cultures of hippocampal neurons and astrocytes. Confocal and electron microscopy of mouse hippocampal sections confirmed the mitochondrial localization of Sab in the soma, dendrites, and axons. Given the localization and sub-neuronal distribution of Sab, we postulated that Sab-mediated signaling could affect neuronal function, so we measured the impact of inhibiting Sab-mediated events on the spontaneous activity in cultured hippocampal neurons. Treatment with a Sab-inhibitory peptide (Tat-SabKIM1), but not a scrambled control peptide, decreased the firing frequency and spike amplitudes. Our results demonstrate that brain-specific Sab-mediated signaling plays a role in neuronal activity through the manipulation of mitochondrial physiology by interacting kinases.
format Artículo
author Sodero, Alejandro Omar
Rodriguez-Silva, Mónica
Salio, Chiara
Sassoè-Pognetto, Marco
Chambers, Jeremy W.
author_facet Sodero, Alejandro Omar
Rodriguez-Silva, Mónica
Salio, Chiara
Sassoè-Pognetto, Marco
Chambers, Jeremy W.
author_sort Sodero, Alejandro Omar
title Sab is differentially expressed in the brain and affects neuronal activity
title_short Sab is differentially expressed in the brain and affects neuronal activity
title_full Sab is differentially expressed in the brain and affects neuronal activity
title_fullStr Sab is differentially expressed in the brain and affects neuronal activity
title_full_unstemmed Sab is differentially expressed in the brain and affects neuronal activity
title_sort sab is differentially expressed in the brain and affects neuronal activity
publisher Elsevier
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/8567
work_keys_str_mv AT soderoalejandroomar sabisdifferentiallyexpressedinthebrainandaffectsneuronalactivity
AT rodriguezsilvamonica sabisdifferentiallyexpressedinthebrainandaffectsneuronalactivity
AT saliochiara sabisdifferentiallyexpressedinthebrainandaffectsneuronalactivity
AT sassoepognettomarco sabisdifferentiallyexpressedinthebrainandaffectsneuronalactivity
AT chambersjeremyw sabisdifferentiallyexpressedinthebrainandaffectsneuronalactivity
bdutipo_str Repositorios
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