Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain

Abstract: Perinatal asphyxia (PA) is an obstetric complication associated with an impaired gas exchange. This health problem continues to be a determinant of neonatal mortality and neurodevelopmental disorders. Palmitoylethanolamide (PEA) has exerted neuroprotection in several models of brain injury...

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Detalles Bibliográficos
Autores principales: Herrera, María Inés, Udovin, Lucas Daniel, Toro-Urrego, Nicolás, Kusnier, Carlos Federico, Luaces, Juan P., Capani, Francisco
Formato: Artículo
Lenguaje:Inglés
Publicado: Frontiers Media 2019
Materias:
COMPORTAMIENTO
ASFIXIA PRENATAL
NEUROPSICOLOGIA
TRASTORNOS DEL NEURODESARROLLO
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/6220
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-6220
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic COMPORTAMIENTO
ASFIXIA PRENATAL
NEUROPSICOLOGIA
TRASTORNOS DEL NEURODESARROLLO
spellingShingle COMPORTAMIENTO
ASFIXIA PRENATAL
NEUROPSICOLOGIA
TRASTORNOS DEL NEURODESARROLLO
Herrera, María Inés
Udovin, Lucas Daniel
Toro-Urrego, Nicolás
Kusnier, Carlos Federico
Luaces, Juan P.
Capani, Francisco
Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain
topic_facet COMPORTAMIENTO
ASFIXIA PRENATAL
NEUROPSICOLOGIA
TRASTORNOS DEL NEURODESARROLLO
description Abstract: Perinatal asphyxia (PA) is an obstetric complication associated with an impaired gas exchange. This health problem continues to be a determinant of neonatal mortality and neurodevelopmental disorders. Palmitoylethanolamide (PEA) has exerted neuroprotection in several models of brain injury and neurodegeneration. We aimed at evaluating the potential neuroprotective role of PEA in an experimental model, which induces PA in the immature rat brain. PA was induced by placing Sprague Dawley newborn rats in a water bath at 37°C for 19 min. Once their physiological conditions improved, they were given to surrogate mothers that had delivered normally within the last 24 h. The control group was represented by non-fostered vaginally delivered pups, mimicking the clinical situation. Treatment with PEA (10 mg/kg) was administered within the first hour of life. Modifications in the hippocampus were analyzed with conventional electron microscopy, immunohistochemistry (for NeuN, pNF-H/M, MAP-2, and GFAP) and western blot (for pNF H/M, MAP-2, and GFAP). Behavior was also studied throughout Open Field (OF) Test, Passive Avoidance (PA) Task and Elevated Plus Maze (EPM) Test. After 1 month of the PA insult, we observed neuronal nucleus degeneration in CA1 using electron microscopy. Immunohistochemistry revealed a significant increase in pNF-H/M and decrease in MAP-2 in CA1 reactive area. These changes were also observed when analyzing the level of expression of these markers by western blot. Vertical exploration impairments and anxiety-related behaviors were encountered in the OF and EPM tests. PEA treatment attenuated PA-induced hippocampal damage and its corresponding behavioral alterations. These results contribute to the elucidation of PEA neuroprotective role after PA and the future establishment of therapeutic strategies for the developing brain.
format Artículo
author Herrera, María Inés
Udovin, Lucas Daniel
Toro-Urrego, Nicolás
Kusnier, Carlos Federico
Luaces, Juan P.
Capani, Francisco
author_facet Herrera, María Inés
Udovin, Lucas Daniel
Toro-Urrego, Nicolás
Kusnier, Carlos Federico
Luaces, Juan P.
Capani, Francisco
author_sort Herrera, María Inés
title Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain
title_short Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain
title_full Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain
title_fullStr Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain
title_full_unstemmed Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain
title_sort palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain
publisher Frontiers Media
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/6220
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