Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos

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Abstract: Devitrification, the process of crystallization of a formerly crystal-free, amorphous glass state, can lead to damage during the warming of cells. The objective of this study was to determine the glass transition temperature of a cryopreservation solution typically used in the vitrificatio...

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Autores principales: Sansiñena, Marina Julia, Santos, María Victoria, Taminelli, Guillermo, Zaritzky, Noemí
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2019
Materias:
VITRIFICACION
EMBRIONES
NITROGENO LIQUIDO
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/5495
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-5495
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic VITRIFICACION
EMBRIONES
NITROGENO LIQUIDO
spellingShingle VITRIFICACION
EMBRIONES
NITROGENO LIQUIDO
Sansiñena, Marina Julia
Santos, María Victoria
Taminelli, Guillermo
Zaritzky, Noemí
Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos
topic_facet VITRIFICACION
EMBRIONES
NITROGENO LIQUIDO
description Abstract: Devitrification, the process of crystallization of a formerly crystal-free, amorphous glass state, can lead to damage during the warming of cells. The objective of this study was to determine the glass transition temperature of a cryopreservation solution typically used in the vitrification, storage and warming of mammalian oocytes and embryos using Differential Scanning Calorimetry. A numerical model of the heat transfer process to analyze warming and devitrification thresholds for a common vitrification carrier (open pulled straw, OPS) was conducted. The implications on specimen handling and storage inside the dewar in contact with nitrogen vapor phase at different temperatures were determined. The time required for initiation of devitrification of a vitrified sample was determined by mathematical modeling and compared with measured temperatures in the vapor phase of liquid nitrogen cryogenic dewars. Results indicated that the glass transition ranged from -126 to -121ºC and devitrification was initiated at -109ºC. Interestingly, samples entered rubbery state at -121ºC and therefore could potentially initiate devitrification above this value, with the consequent damaging effects to cell survival. Devitrification times were calculated considering an initial temperature of material immersed in liquid nitrogen (-196ºC) and two temperatures of liquid nitrogen vapors within the dewar (-50 and -70ºC) to which the sample could be exposed for a period of time, either during storage or upon its removal. The mathematical model indicated samples could reach glass transition temperatures and undergo devitrification in 30 seconds. Results of the present study indicate storage of vitrified oocytes and embryos in the liquid nitrogen vapor phase (as opposed to completely immersed in liquid nitrogen) poses the potential risk of devitrification. Due to the reduced time-handling period before samples reach critical rubbery and devitrification values, caution should be exercised when handling samples in vapor phase
format Artículo
author Sansiñena, Marina Julia
Santos, María Victoria
Taminelli, Guillermo
Zaritzky, Noemí
author_facet Sansiñena, Marina Julia
Santos, María Victoria
Taminelli, Guillermo
Zaritzky, Noemí
author_sort Sansiñena, Marina Julia
title Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos
title_short Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos
title_full Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos
title_fullStr Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos
title_full_unstemmed Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos
title_sort implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos
publisher Elsevier
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/5495
work_keys_str_mv AT sansinenamarinajulia implicationsofstorageandhandlingconditionsonglasstransitionandpotentialdevitrificationofoocytesandembryos
AT santosmariavictoria implicationsofstorageandhandlingconditionsonglasstransitionandpotentialdevitrificationofoocytesandembryos
AT taminelliguillermo implicationsofstorageandhandlingconditionsonglasstransitionandpotentialdevitrificationofoocytesandembryos
AT zaritzkynoemi implicationsofstorageandhandlingconditionsonglasstransitionandpotentialdevitrificationofoocytesandembryos
bdutipo_str Repositorios
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