How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor?

Abstract: The subject of present work was to predict internal temperature gradients developed during freezing of bovine sperm diluted in extender, packaged in 0.5 ml French plastic straws and suspended in static liquid nitrogen vapor at -100 ºC. For this purpose, a mathematical heat transfer model p...

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Autores principales: Santos, María Victoria, Sansiñena, Marina Julia, Zaritzky, Noemí, Chirife, Jorge
Formato: Artículo
Lenguaje:Inglés
Publicado: Ingenta 2019
Materias:
CONGELACION
ESPERMA BOVINO
TRANSFERENCIA DE CALOR
VAPOR DE NITROGENO
COEFICIENTE
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/5491
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-5491
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic CONGELACION
ESPERMA BOVINO
TRANSFERENCIA DE CALOR
VAPOR DE NITROGENO
COEFICIENTE
spellingShingle CONGELACION
ESPERMA BOVINO
TRANSFERENCIA DE CALOR
VAPOR DE NITROGENO
COEFICIENTE
Santos, María Victoria
Sansiñena, Marina Julia
Zaritzky, Noemí
Chirife, Jorge
How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor?
topic_facet CONGELACION
ESPERMA BOVINO
TRANSFERENCIA DE CALOR
VAPOR DE NITROGENO
COEFICIENTE
description Abstract: The subject of present work was to predict internal temperature gradients developed during freezing of bovine sperm diluted in extender, packaged in 0.5 ml French plastic straws and suspended in static liquid nitrogen vapor at -100 ºC. For this purpose, a mathematical heat transfer model previously developed to predict freezing times (phase change was considered) of semen/extender packaged in straw was extended to predict internal temperature gradients during the cooling/freezing process. Results showed maximum temperature differences between the centre and the periphery of semen/extender “liquid” column was 1.5 ºC for an external heat transfer coefficient, h = 15 W/(m2 K), and only 0.5 ºC for h = 5 W/(m2 K). It is concluded that if a thermocouple wire were inserted in a 0.5 ml plastic straw to monitor the freezing process in nitrogen vapor, its radial position would have little importance since expected internal gradients may be safely neglected. This finding facilitates the interpretation of freezing rates in 0.5 ml plastic straws immersed in nitrogen vapor over liquid nitrogen, a widely used method for cryopreservation of bovine spermatozoa
format Artículo
author Santos, María Victoria
Sansiñena, Marina Julia
Zaritzky, Noemí
Chirife, Jorge
author_facet Santos, María Victoria
Sansiñena, Marina Julia
Zaritzky, Noemí
Chirife, Jorge
author_sort Santos, María Victoria
title How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor?
title_short How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor?
title_full How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor?
title_fullStr How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor?
title_full_unstemmed How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor?
title_sort how important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor?
publisher Ingenta
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/5491
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