Implications of glass transition in the devitrification process and storage management of vitrified oocytes and embryos

Abstract: Devitrification, the process of crystallization of a formerly crystal-free, amorphous glass state, can lead to damage during the warming of cells. The objective of this study was to determine the glass transition temperature of a cryopreservation solution typically used in the vitrificatio...

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Autores principales: Sansiñena, Marina Julia, Zaritzky, Noemí, Taminelli, Guillermo, Santos, María Victoria
Otros Autores: Universidad Católica Argentina. Facultad de Ciencias Agrarias
Formato: Artículo
Lenguaje:Inglés
Publicado: 2019
Materias:
VITRIFICACION
EMBRIONES
NITROGENO LIQUIDO
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/5487
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-5487
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic VITRIFICACION
EMBRIONES
NITROGENO LIQUIDO
spellingShingle VITRIFICACION
EMBRIONES
NITROGENO LIQUIDO
Sansiñena, Marina Julia
Zaritzky, Noemí
Taminelli, Guillermo
Santos, María Victoria
Implications of glass transition in the devitrification process and storage management of vitrified oocytes and embryos
topic_facet VITRIFICACION
EMBRIONES
NITROGENO LIQUIDO
description Abstract: Devitrification, the process of crystallization of a formerly crystal-free, amorphous glass state, can lead to damage during the warming of cells. The objective of this study was to determine the glass transition temperature of a cryopreservation solution typically used in the vitrification, storage and warming of mammalian oocytes and embryos using Differential Scanning Calorimetry. A numerical model of the heat transfer process to analyze warming and devitrification thresholds for a vitrification carrier (open-pulled straw, OPS) was conducted and the implications on specimen storage in nitrogen vapor phase were determined. The time required for initiation of devitrification was determined by mathematical modeling and compared with temperatures in the vapor phase of liquid nitrogen cryogenic dewars. Results indicated that the glass transition ranged from -126 to -121ºC and devitrification was initiated at -109ºC. Interestingly, samples entered rubbery state at -121ºC and therefore could potentially initiate devitrification above this value, with the consequent damaging effects to cell survival. Devitrification itimes were mathematically modeled considering an initial temperature of material immersed in liquid nitrogen (-196ºC) and two arbitrarily selected temperatures (-50 and -70ºC) to which a sample could be exposed at the neck of dewar. The mathematical model indicated samples could reach glass transition temperatures and undergo devitrification in 30 seconds. Results of the present study indicate storage of vitrified oocytes and embryos in the liquid nitrogen vapor phase (as opposed to completely immersed in liquid nitrogen) poses the potential risk of devitrification. Due to the reduced time handling period before samples reach critical rubbery and devitrification values, caution should be exercised when handling samples in vapor phase
author2 Universidad Católica Argentina. Facultad de Ciencias Agrarias
author_facet Universidad Católica Argentina. Facultad de Ciencias Agrarias
Sansiñena, Marina Julia
Zaritzky, Noemí
Taminelli, Guillermo
Santos, María Victoria
format Artículo
author Sansiñena, Marina Julia
Zaritzky, Noemí
Taminelli, Guillermo
Santos, María Victoria
author_sort Sansiñena, Marina Julia
title Implications of glass transition in the devitrification process and storage management of vitrified oocytes and embryos
title_short Implications of glass transition in the devitrification process and storage management of vitrified oocytes and embryos
title_full Implications of glass transition in the devitrification process and storage management of vitrified oocytes and embryos
title_fullStr Implications of glass transition in the devitrification process and storage management of vitrified oocytes and embryos
title_full_unstemmed Implications of glass transition in the devitrification process and storage management of vitrified oocytes and embryos
title_sort implications of glass transition in the devitrification process and storage management of vitrified oocytes and embryos
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/5487
work_keys_str_mv AT sansinenamarinajulia implicationsofglasstransitioninthedevitrificationprocessandstoragemanagementofvitrifiedoocytesandembryos
AT zaritzkynoemi implicationsofglasstransitioninthedevitrificationprocessandstoragemanagementofvitrifiedoocytesandembryos
AT taminelliguillermo implicationsofglasstransitioninthedevitrificationprocessandstoragemanagementofvitrifiedoocytesandembryos
AT santosmariavictoria implicationsofglasstransitioninthedevitrificationprocessandstoragemanagementofvitrifiedoocytesandembryos
bdutipo_str Repositorios
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