Numerical simulation of cooling rates in vitrification systems used for oocyte cryopreservation

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Abstract: Oocyte cryopreservation is of key importance in the preservation and propagation of germplasm. Interest 24 in oocyte cryopreservation has increased in recent years due to the application of assisted reproductive 25 technologies in farm animals such as in vitro fertilization, nuclear transf...

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Autores principales: Sansiñena, Marina Julia, Santos, María Victoria, Zaritzky, Noemí, Chirife, Jorge
Otros Autores: Universidad Católica Argentina. Facultad de Ciencias Agrarias
Formato: Artículo
Lenguaje:Inglés
Publicado: 2019
Materias:
ENFRIAMIENTO
OVOCITOS
TRANSFERENCIA DE CALOR
METODO DE ELEMENTOS FINITOS
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/5467
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-5467
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic ENFRIAMIENTO
OVOCITOS
TRANSFERENCIA DE CALOR
METODO DE ELEMENTOS FINITOS
spellingShingle ENFRIAMIENTO
OVOCITOS
TRANSFERENCIA DE CALOR
METODO DE ELEMENTOS FINITOS
Sansiñena, Marina Julia
Santos, María Victoria
Zaritzky, Noemí
Chirife, Jorge
Numerical simulation of cooling rates in vitrification systems used for oocyte cryopreservation
topic_facet ENFRIAMIENTO
OVOCITOS
TRANSFERENCIA DE CALOR
METODO DE ELEMENTOS FINITOS
description Abstract: Oocyte cryopreservation is of key importance in the preservation and propagation of germplasm. Interest 24 in oocyte cryopreservation has increased in recent years due to the application of assisted reproductive 25 technologies in farm animals such as in vitro fertilization, nuclear transfer and the need for the establish- 26 ment of ova/gene banks worldwide. However, the cryopreservation of the female gamete has been met 27 with limited success mainly due to its small surface-area:volume ratio. 28 In the past decade, several vitrification devices such as open pulled straws (OPS), fine and ultra fine pip- 29 ette tips, nylon loops and polyethylene films have been introduced in order to manipulate minimal vol- 30 umes and achieve high cooling rates. However, experimental comparison of cooling rates presents 31 difficulties mainly because of the reduced size of these systems. To circumvent this limitation, a numer- 32 ical simulation of cooling rates of various vitrification systems immersed in liquid nitrogen was con- 33 ducted solving the non-stationary heat transfer partial differential equation using finite element method. 34 Results indicate the nylon loop (Cryoloop) is the most efficient heat transfer system analyzed, with a 35 predicted cooling rate of 180,000 C/min for an external heat transfer coefficient h = 1000W/m2 K when 36 cooling from 20 to 130 C; in contrast, the open pulled straw method (OPS) showed the lowest perfor- 37 mance with a cooling rate of 5521 C/min considering the same value of external heat transfer coefficient. 38 Predicted cooling rates of Miniflex and Cryotop (polyethylene film system) were 6164 and 37,500 C/ 39 min, respectively, for the same heat transfer coefficient
author2 Universidad Católica Argentina. Facultad de Ciencias Agrarias
author_facet Universidad Católica Argentina. Facultad de Ciencias Agrarias
Sansiñena, Marina Julia
Santos, María Victoria
Zaritzky, Noemí
Chirife, Jorge
format Artículo
author Sansiñena, Marina Julia
Santos, María Victoria
Zaritzky, Noemí
Chirife, Jorge
author_sort Sansiñena, Marina Julia
title Numerical simulation of cooling rates in vitrification systems used for oocyte cryopreservation
title_short Numerical simulation of cooling rates in vitrification systems used for oocyte cryopreservation
title_full Numerical simulation of cooling rates in vitrification systems used for oocyte cryopreservation
title_fullStr Numerical simulation of cooling rates in vitrification systems used for oocyte cryopreservation
title_full_unstemmed Numerical simulation of cooling rates in vitrification systems used for oocyte cryopreservation
title_sort numerical simulation of cooling rates in vitrification systems used for oocyte cryopreservation
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/5467
work_keys_str_mv AT sansinenamarinajulia numericalsimulationofcoolingratesinvitrificationsystemsusedforoocytecryopreservation
AT santosmariavictoria numericalsimulationofcoolingratesinvitrificationsystemsusedforoocytecryopreservation
AT zaritzkynoemi numericalsimulationofcoolingratesinvitrificationsystemsusedforoocytecryopreservation
AT chirifejorge numericalsimulationofcoolingratesinvitrificationsystemsusedforoocytecryopreservation
bdutipo_str Repositorios
_version_ 1764820527369682945

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