Fluctuating temperatures terminate dormancy in cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA synthesis or signalling

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Abstract: Fluctuating temperatures terminate seed dormancy in many species, including Cynara cardunculus (L.) var. sylvestris. Termination of physiological dormancy requires low ratios of abscisic acid (ABA)/gibberellins (GA). In a previous paper we have shown that physiological responses to fluctua...

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Autores principales: Huarte, Héctor Roberto, Luna, Virginia, Pagano, Eduardo A., Zavala, Jorge A., Benech Amold, Roberto L.
Otros Autores: Universidad Católica Argentina (Buenos Aires). Facultad de Ciencias Agrarias
Formato: Artículo
Lenguaje:Inglés
Publicado: 2019
Materias:
ACIDO ABSCISICO
INACTIVIDAD
TEMPERATURA
EXPRESION GENICA
GIBERELINAS
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/5463
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-5463
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic ACIDO ABSCISICO
INACTIVIDAD
TEMPERATURA
EXPRESION GENICA
GIBERELINAS
spellingShingle ACIDO ABSCISICO
INACTIVIDAD
TEMPERATURA
EXPRESION GENICA
GIBERELINAS
Huarte, Héctor Roberto
Luna, Virginia
Pagano, Eduardo A.
Zavala, Jorge A.
Benech Amold, Roberto L.
Fluctuating temperatures terminate dormancy in cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA synthesis or signalling
topic_facet ACIDO ABSCISICO
INACTIVIDAD
TEMPERATURA
EXPRESION GENICA
GIBERELINAS
description Abstract: Fluctuating temperatures terminate seed dormancy in many species, including Cynara cardunculus (L.) var. sylvestris. Termination of physiological dormancy requires low ratios of abscisic acid (ABA)/gibberellins (GA). In a previous paper we have shown that physiological responses to fluctuating temperatures comprise a reduction of abscisic acid (ABA) content and sensitivity. However, a possible stimulation of GA synthesis was also suggested as part of the mechanism. That possible stimulation, as well as the identification of potential regulatory sites for ABA and GA metabolism and signalling involved in the termination of dormancy by fluctuating temperatures, are yet to be determined. In this study, we measured GA content and sensitivity in seeds incubated under constant and fluctuating temperatures. We also assessed the expression of several genes involved in ABA and GA metabolism and signalling. Our results show that fluctuating temperatures reduce ABA/GA ratios through a reduction in ABA accumulation during incubation but without altering GA synthesis as compared to that observed under constant temperatures. On the other hand, fluctuating temperatures did not increase sensitivity to GA. Fluctuating temperatures reduced the expression of CycaNCED and CycaABI5 (ABA synthesis and signalling genes) with a temporal pattern that coincides with the interruption of ABA accumulation that precedes germination of seeds incubated under fluctuating temperatures. However, fluctuating temperatures did not modify the expression of CycaCYP707A2 (ABA inactivation) as compared to that observed under constant temperatures. Consistent with our determinations of GA content and sensitivity, fluctuating temperatures did not modify the expression of GA synthesis (CycaGA3ox) and signalling genes (CycaRGL2 and CycaGAI) in relation to that observed at constant temperatures. These results show that fluctuating temperatures terminate dormancy in Cynara cardunculus seeds through an interruption in ABA accumulation and a reduction in ABA signalling exerted at the level of CycaNCED and CycaABI5 expression
author2 Universidad Católica Argentina (Buenos Aires). Facultad de Ciencias Agrarias
author_facet Universidad Católica Argentina (Buenos Aires). Facultad de Ciencias Agrarias
Huarte, Héctor Roberto
Luna, Virginia
Pagano, Eduardo A.
Zavala, Jorge A.
Benech Amold, Roberto L.
format Artículo
author Huarte, Héctor Roberto
Luna, Virginia
Pagano, Eduardo A.
Zavala, Jorge A.
Benech Amold, Roberto L.
author_sort Huarte, Héctor Roberto
title Fluctuating temperatures terminate dormancy in cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA synthesis or signalling
title_short Fluctuating temperatures terminate dormancy in cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA synthesis or signalling
title_full Fluctuating temperatures terminate dormancy in cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA synthesis or signalling
title_fullStr Fluctuating temperatures terminate dormancy in cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA synthesis or signalling
title_full_unstemmed Fluctuating temperatures terminate dormancy in cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA synthesis or signalling
title_sort fluctuating temperatures terminate dormancy in cynara cardunculus seeds by turning off aba synthesis and reducing aba signalling, but not stimulating ga synthesis or signalling
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/5463
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