Determination of heat transfer coefficients in plastic French straws plunged in liquid nitrogen

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Abstract: The knowledge of the thermodynamic process during the cooling of reproductive biological systems is important to assess and optimize the cryopreservation procedures. The time temperature curve of a sample immersed in liquid nitrogen enables the calculation of cooling rates and helps to det...

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Autores principales: Santos, María Victoria, Sansiñena, Marina Julia, Chirife, Jorge, Zaritzky, Noemí
Otros Autores: Universidad Nacional de La Plata (Argentina). Facultad de Ingeniería. Departamento de Ingeniería Química
Formato: Artículo
Lenguaje:Inglés
Publicado: 2019
Materias:
TRANSFERENCIA DE CALOR
NITROGENO LIQUIDO
CONGELACION
REFRIGERACION
METODO DE ELEMENTOS FINITOS
EBULLICION
CRIOPRESERVACION
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/5457
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-5457
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic TRANSFERENCIA DE CALOR
NITROGENO LIQUIDO
CONGELACION
REFRIGERACION
METODO DE ELEMENTOS FINITOS
EBULLICION
CRIOPRESERVACION
spellingShingle TRANSFERENCIA DE CALOR
NITROGENO LIQUIDO
CONGELACION
REFRIGERACION
METODO DE ELEMENTOS FINITOS
EBULLICION
CRIOPRESERVACION
Santos, María Victoria
Sansiñena, Marina Julia
Chirife, Jorge
Zaritzky, Noemí
Determination of heat transfer coefficients in plastic French straws plunged in liquid nitrogen
topic_facet TRANSFERENCIA DE CALOR
NITROGENO LIQUIDO
CONGELACION
REFRIGERACION
METODO DE ELEMENTOS FINITOS
EBULLICION
CRIOPRESERVACION
description Abstract: The knowledge of the thermodynamic process during the cooling of reproductive biological systems is important to assess and optimize the cryopreservation procedures. The time temperature curve of a sample immersed in liquid nitrogen enables the calculation of cooling rates and helps to determine whether it is vitrified or undergoes phase change transition. When dealing with cryogenic liquids, the temperature difference between the solid and the sample is high enough to cause boiling of the liquid, and the sample can undergo different regimes such as film and/or nucleate pool boiling. In the present work, the surface heat transfer coefficients (h) for plastic French straws plunged in liquid nitrogen were determined using the measurement of time-temperature curves. When straws filled with ice were used the cooling curve showed an abrupt slope change which was attributed to the transition of film into nucleate pool boiling regime. The h value that fitted each stage of the cooling process was calculated using a numerical finite element program that solves the heat transfer partial differential equation under transient conditions. In the cooling process corresponding to film boiling regime, the h that best fitted experimental results was h=148.12 ± 5.4 W/m2 K and for nucleate-boiling h=1355 ± 51 W/m2 K. These values were further validated by predicting the time-temperature curve for French straws filled with a biological fluid system (bovine semen-extender) which undergoes freezing. Good agreement was obtained between the experimental and predicted temperature profiles, further confirming the accuracy of the h values previously determined for the ice-filled straw. These coefficients were corroborated using literature correlations. The determination of the boiling regimes that govern the cooling process when plunging straws in liquid nitrogen constitutes an important issue when trying to optimize cryopreservation procedures. Furthermore, this information can lead to improvements in the design of cooling devices in the cryobiology field
author2 Universidad Nacional de La Plata (Argentina). Facultad de Ingeniería. Departamento de Ingeniería Química
author_facet Universidad Nacional de La Plata (Argentina). Facultad de Ingeniería. Departamento de Ingeniería Química
Santos, María Victoria
Sansiñena, Marina Julia
Chirife, Jorge
Zaritzky, Noemí
format Artículo
author Santos, María Victoria
Sansiñena, Marina Julia
Chirife, Jorge
Zaritzky, Noemí
author_sort Santos, María Victoria
title Determination of heat transfer coefficients in plastic French straws plunged in liquid nitrogen
title_short Determination of heat transfer coefficients in plastic French straws plunged in liquid nitrogen
title_full Determination of heat transfer coefficients in plastic French straws plunged in liquid nitrogen
title_fullStr Determination of heat transfer coefficients in plastic French straws plunged in liquid nitrogen
title_full_unstemmed Determination of heat transfer coefficients in plastic French straws plunged in liquid nitrogen
title_sort determination of heat transfer coefficients in plastic french straws plunged in liquid nitrogen
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/5457
work_keys_str_mv AT santosmariavictoria determinationofheattransfercoefficientsinplasticfrenchstrawsplungedinliquidnitrogen
AT sansinenamarinajulia determinationofheattransfercoefficientsinplasticfrenchstrawsplungedinliquidnitrogen
AT chirifejorge determinationofheattransfercoefficientsinplasticfrenchstrawsplungedinliquidnitrogen
AT zaritzkynoemi determinationofheattransfercoefficientsinplasticfrenchstrawsplungedinliquidnitrogen
bdutipo_str Repositorios
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