24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment
Abstract: The 24-h changes in medial basal hypothalamic (MBH) gene expression of redox pathway enzymes nitric oxide synthase (NOS)-1 and NOS-2, heme oxygenase (HO)-1 and HO-2, Cu/Znand Mn-superoxide dismutases (SOD) and catalase were examined in adult male Wistar rats. Half of the animals received m...
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Formato: | Artículo |
Lenguaje: | Inglés Inglés |
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Taylor & Francis
2019
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Acceso en línea: | https://repositorio.uca.edu.ar/handle/123456789/1626 |
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I33-R139123456789-1626 |
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institution |
Universidad Católica Argentina |
institution_str |
I-33 |
repository_str |
R-139 |
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Repositorio Institucional de la Universidad Católica Argentina (UCA) |
language |
Inglés Inglés |
topic |
MELATONINA RITMO CIRCADIANO HIPOTALAMO MEDIO EXPRESION DE GENES CATALASA OXIDO NITRICO SINTASA |
spellingShingle |
MELATONINA RITMO CIRCADIANO HIPOTALAMO MEDIO EXPRESION DE GENES CATALASA OXIDO NITRICO SINTASA Jiménez Ortega, Vanesa Cano Barquilla, Pilar Cardinali, Daniel Pedro Esquifino, Ana I. 24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment |
topic_facet |
MELATONINA RITMO CIRCADIANO HIPOTALAMO MEDIO EXPRESION DE GENES CATALASA OXIDO NITRICO SINTASA |
description |
Abstract: The 24-h changes in medial basal hypothalamic (MBH) gene expression of redox pathway enzymes nitric oxide synthase (NOS)-1 and NOS-2, heme oxygenase (HO)-1 and HO-2, Cu/Znand Mn-superoxide dismutases (SOD) and catalase were examined in adult male Wistar rats. Half of the animals received melatonin (ca. 60 μg/day) in the drinking water. After 1 month, rats were killed at six different time intervals, throughout a 24-h cycle. MBH mRNA levels were measured by real-time PCR analysis. In controls NOS-1 mRNA expression did not exhibit 24-h variations while NOS-2 gene expression peaked at the early light phase of cycle. MBH mRNA for both the inducible (HO-1) and constitutive (HO-2) isoforms of HO showed a significant 24 h pattern with a maximum at the middle of the dark phase or at the early light phase, respectively. None of mRNAs encoding Cu/Zn-SOD, Mn-SOD and catalase exhibited significant 24-h variations in rat MBH under control conditions. Chronic melatonin administration in the drinking water decreased significantly mRNAs for NOS-1, NOS-2, HO-1 and HO-2 as well as changed their 24-h profile. Melatonin augmented gene expression of the antioxidant enzymes Cu/Zn-SOD, Mn-SOD or catalase at certain time intervals only |
format |
Artículo |
author |
Jiménez Ortega, Vanesa Cano Barquilla, Pilar Cardinali, Daniel Pedro Esquifino, Ana I. |
author_facet |
Jiménez Ortega, Vanesa Cano Barquilla, Pilar Cardinali, Daniel Pedro Esquifino, Ana I. |
author_sort |
Jiménez Ortega, Vanesa |
title |
24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment |
title_short |
24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment |
title_full |
24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment |
title_fullStr |
24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment |
title_full_unstemmed |
24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment |
title_sort |
24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment |
publisher |
Taylor & Francis |
publishDate |
2019 |
url |
https://repositorio.uca.edu.ar/handle/123456789/1626 |
work_keys_str_mv |
AT jimenezortegavanesa 24hourrhythmingeneexpressionofredoxpathwayenzymesinrathypothalamuseffectofmelatonintreatment AT canobarquillapilar 24hourrhythmingeneexpressionofredoxpathwayenzymesinrathypothalamuseffectofmelatonintreatment AT cardinalidanielpedro 24hourrhythmingeneexpressionofredoxpathwayenzymesinrathypothalamuseffectofmelatonintreatment AT esquifinoanai 24hourrhythmingeneexpressionofredoxpathwayenzymesinrathypothalamuseffectofmelatonintreatment |
bdutipo_str |
Repositorios |
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1764820525748584449 |