Effect of a high-fat diet on 24-hour pattern in expression of prolactin and redox pathway enzymes in the rat adenohypophysis

Abstract: The effect of a high-fat diet (35% fat) on 24-h changes in expression of prolactin and redox pathway enzyme genes was examined in the anterior pituitary of male rats. When body weight of high-fat fed rats attained values about 20- 25 % higher than controls (after 66 days of treatment) anim...

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Autores principales: Cano Barquilla, Pilar, Cardinali, Daniel Pedro, Jiménez Ortega, Vanesa, Scacchi, Pablo, Esquifino, Ana I.
Formato: Artículo
Lenguaje:Inglés
Inglés
Publicado: 2019
Materias:
DIETA RICA EN GRASAS
RITMO CIRCADIANO
EXPRESION GENICA
PROLACTINA
OXIDO NITRICO SINTASA
HEMO-OXIGENASA
CATALASA
SUPEROXIDO DISMUTASA
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/1616
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-1616
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
Inglés
topic DIETA RICA EN GRASAS
RITMO CIRCADIANO
EXPRESION GENICA
PROLACTINA
OXIDO NITRICO SINTASA
HEMO-OXIGENASA
CATALASA
SUPEROXIDO DISMUTASA
spellingShingle DIETA RICA EN GRASAS
RITMO CIRCADIANO
EXPRESION GENICA
PROLACTINA
OXIDO NITRICO SINTASA
HEMO-OXIGENASA
CATALASA
SUPEROXIDO DISMUTASA
Cano Barquilla, Pilar
Cardinali, Daniel Pedro
Jiménez Ortega, Vanesa
Scacchi, Pablo
Esquifino, Ana I.
Effect of a high-fat diet on 24-hour pattern in expression of prolactin and redox pathway enzymes in the rat adenohypophysis
topic_facet DIETA RICA EN GRASAS
RITMO CIRCADIANO
EXPRESION GENICA
PROLACTINA
OXIDO NITRICO SINTASA
HEMO-OXIGENASA
CATALASA
SUPEROXIDO DISMUTASA
description Abstract: The effect of a high-fat diet (35% fat) on 24-h changes in expression of prolactin and redox pathway enzyme genes was examined in the anterior pituitary of male rats. When body weight of high-fat fed rats attained values about 20- 25 % higher than controls (after 66 days of treatment) animals were sacrificed at 6 different time intervals throughout a 24-h cycle. Anterior pituitary mRNA levels were measured by real-time PCR analysis. In control rats expression of the prolactin gene peaked in anterior pituitary at early scotophase, 3 h in advance to the peak in plasma prolactin levels. In high-fat fed rats this correlation was lost. A disruption of 24-h rhythmicity of pituitary gene expression of heme oxygenase (HO)-2, Cu/Zn- superoxide dismutases (SOD), Mn-SOD, catalase, glutathione peroxidase (GPx) and glutathione reductase (GR) was apparent in high-fat fed rats. Anterior pituitary mRNA levels for nitric oxide synthase (NOS)-2 and HO-2, Cu/Zn- and Mn- SOD and catalase augmented in high-fat fed rats, whereas those of GPx and GR decreased. The results indicate a disrupted coordination between prolactin gene expression and prolactin release in highfat fed rats that comes along with a disturbed expression of redox enzyme genes in the anterior pituitary.
format Artículo
author Cano Barquilla, Pilar
Cardinali, Daniel Pedro
Jiménez Ortega, Vanesa
Scacchi, Pablo
Esquifino, Ana I.
author_facet Cano Barquilla, Pilar
Cardinali, Daniel Pedro
Jiménez Ortega, Vanesa
Scacchi, Pablo
Esquifino, Ana I.
author_sort Cano Barquilla, Pilar
title Effect of a high-fat diet on 24-hour pattern in expression of prolactin and redox pathway enzymes in the rat adenohypophysis
title_short Effect of a high-fat diet on 24-hour pattern in expression of prolactin and redox pathway enzymes in the rat adenohypophysis
title_full Effect of a high-fat diet on 24-hour pattern in expression of prolactin and redox pathway enzymes in the rat adenohypophysis
title_fullStr Effect of a high-fat diet on 24-hour pattern in expression of prolactin and redox pathway enzymes in the rat adenohypophysis
title_full_unstemmed Effect of a high-fat diet on 24-hour pattern in expression of prolactin and redox pathway enzymes in the rat adenohypophysis
title_sort effect of a high-fat diet on 24-hour pattern in expression of prolactin and redox pathway enzymes in the rat adenohypophysis
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/1616
work_keys_str_mv AT canobarquillapilar effectofahighfatdieton24hourpatterninexpressionofprolactinandredoxpathwayenzymesintheratadenohypophysis
AT cardinalidanielpedro effectofahighfatdieton24hourpatterninexpressionofprolactinandredoxpathwayenzymesintheratadenohypophysis
AT jimenezortegavanesa effectofahighfatdieton24hourpatterninexpressionofprolactinandredoxpathwayenzymesintheratadenohypophysis
AT scacchipablo effectofahighfatdieton24hourpatterninexpressionofprolactinandredoxpathwayenzymesintheratadenohypophysis
AT esquifinoanai effectofahighfatdieton24hourpatterninexpressionofprolactinandredoxpathwayenzymesintheratadenohypophysis
bdutipo_str Repositorios
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