Granulocyte colony-stimulating factor (G-CSF) upregulates β1 integrin and increases migration of human trophoblast Swan 71 cells via PI3K and MAPK activation

Abstract: Multiple cytokines and growth factors expressed at the fetal-maternal interface are involved in the regulation of trophoblast functions and placental growth, but the role of G-CSF has not been completely established. Based on our previous study showing that G-CSF increases the activity of...

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Autores principales: Furmento, Verónica A., Marino, Julieta, Blank, Viviana C., Cayrol, María Florencia, Cremaschi, Graciela A., Aguilar, Rubén C., Roguin, Leonor P.
Formato: Artículo
Lenguaje:Inglés
Inglés
Publicado: Elsevier 2019
Materias:
EMBRION HUMANO
BIOLOGIA
QUIMICA BIOLOGICA
PLACENTA
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/1457
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-1457
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
Inglés
topic EMBRION HUMANO
BIOLOGIA
QUIMICA BIOLOGICA
PLACENTA
spellingShingle EMBRION HUMANO
BIOLOGIA
QUIMICA BIOLOGICA
PLACENTA
Furmento, Verónica A.
Marino, Julieta
Blank, Viviana C.
Cayrol, María Florencia
Cremaschi, Graciela A.
Aguilar, Rubén C.
Roguin, Leonor P.
Granulocyte colony-stimulating factor (G-CSF) upregulates β1 integrin and increases migration of human trophoblast Swan 71 cells via PI3K and MAPK activation
topic_facet EMBRION HUMANO
BIOLOGIA
QUIMICA BIOLOGICA
PLACENTA
description Abstract: Multiple cytokines and growth factors expressed at the fetal-maternal interface are involved in the regulation of trophoblast functions and placental growth, but the role of G-CSF has not been completely established. Based on our previous study showing that G-CSF increases the activity of matrix metalloproteinase-2 and the release of vascular endothelial growth factor in Swan 71 human trophoblast cells, in this work we explore the possible contribution of G-CSF to cell migration and the G-CSF-triggered signaling pathway. We found that G-CSF induced morphological changes on actin cytoskeleton consistent with a migratory cell phenotype. G-CSF also up-regulated the expression levels of β1 integrin and promoted Swan 71 cell migration. By using selective pharmacological inhibitors and dominant negative mutants we showed that PI3K, Erk 1/2 and p38 pathways are required for promoting Swan 71 cell motility. It was also demonstrated that PI3K behaved as an upstream regulator of Erk 1/2 and p38 MAPK. In addition, the increase of β1 integrin expression was dependent on PI3K activation. In conclusion, our results indicate that G-CSF stimulates β1 integrin expression and Swan 71 cell migration by activating PI3K and MAPK signaling pathways, suggesting that G-CSF should be considered as an additional regulatory factor that contributes to a successful embryo implantation and to the placenta development.
format Artículo
author Furmento, Verónica A.
Marino, Julieta
Blank, Viviana C.
Cayrol, María Florencia
Cremaschi, Graciela A.
Aguilar, Rubén C.
Roguin, Leonor P.
author_facet Furmento, Verónica A.
Marino, Julieta
Blank, Viviana C.
Cayrol, María Florencia
Cremaschi, Graciela A.
Aguilar, Rubén C.
Roguin, Leonor P.
author_sort Furmento, Verónica A.
title Granulocyte colony-stimulating factor (G-CSF) upregulates β1 integrin and increases migration of human trophoblast Swan 71 cells via PI3K and MAPK activation
title_short Granulocyte colony-stimulating factor (G-CSF) upregulates β1 integrin and increases migration of human trophoblast Swan 71 cells via PI3K and MAPK activation
title_full Granulocyte colony-stimulating factor (G-CSF) upregulates β1 integrin and increases migration of human trophoblast Swan 71 cells via PI3K and MAPK activation
title_fullStr Granulocyte colony-stimulating factor (G-CSF) upregulates β1 integrin and increases migration of human trophoblast Swan 71 cells via PI3K and MAPK activation
title_full_unstemmed Granulocyte colony-stimulating factor (G-CSF) upregulates β1 integrin and increases migration of human trophoblast Swan 71 cells via PI3K and MAPK activation
title_sort granulocyte colony-stimulating factor (g-csf) upregulates β1 integrin and increases migration of human trophoblast swan 71 cells via pi3k and mapk activation
publisher Elsevier
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/1457
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